VPS15 (Kr3q22.1) , PI3KR4

PI3P synteesi , VPS 15 ja VPS34 , mitä ne tekevät vakuolaaristen proteiinien lajitelujärjstlmään assosioituneina?

Vacuolar Protein Sorting Associated protein 15

VPS15 (Kr. 3q22.3), PIK3R4, Phosphoinositide-3-kinase regulatory subunit 4.

Also known as p150; VPS15

Expression Ubiquitous expression in thyroid (RPKM 9.2), testis (RPKM 8.7) and 25 other tissues See moreOrthologs mouse all

Preferred Names

phosphoinositide 3-kinase regulatory subunit 4

Names

PI3-kinase p150 subunit

PI3-kinase regulatory subunit 4

phosphatidylinositol 3-kinase-associated p150

phosphoinositide 3-kinase adaptor protein

phosphoinositide-3-kinase, regulatory subunit 4, p150

NP_055417.1

• EC 2.7.11.1

Peptidirakenne:

Conserved Domains (8) summary

smart00320
Location:1327 → 1358

WD40; WD40 repeats

smart00220
Location:26 → 309

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG2319
Location:991 → 1358

WD40; WD40 repeat [General function prediction only]

cd13980
Location:25 → 320

STKc_Vps15; Catalytic domain of the Serine/Threonine kinase, Vacuolar protein sorting-associated protein 15

pfam07539
Location:352 → 448

DRIM; Down-regulated in metastasis

sd00044
Location:539 → 566

HEAT; HEAT repeat [structural motif]

sd00039
Location:997 → 1040

7WD40; WD40 repeat [structural motif]

cl02567
Location:985 → 1269

WD40;... WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.

Related articles in PubMed

1. Autophagy dysregulation in Danon disease. Nascimbeni AC, et al. Cell Death Dis, 2017 Jan 19. PMID 28102838, Free PMC Article
2. High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia. Kristensen L, et al. Leuk Res, 2015 Jun. PMID 25840748
3. hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells. Yan Y, et al. Biochem J, 2009 Feb 1. PMID 18957027, Free PMC Article
4. The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Ligon LA, et al. Mol Biol Cell, 2003 Apr. PMID 12686597, Free PMC Article
5. Signal transduction pathways mediated by the interaction of CpG DNA with Toll-like receptor 9. Takeshita F, et al. Semin Immunol, 2004 Feb. PMID 14751759
   

See all (37) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. Both Danon Disease and glycogen storage disease type II show accumulation and altered localization of VPS15 in autophagy-incompetent fibers. However, TFEB displays a different pattern between these two lysosomal storage diseases
2. High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia.
3. A specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates both receptor degradation and cytokinesis, whereas ATG14L, a PI3K-III subunit involved in autophagy, is not required.
4. hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells
   

Rakenne ja historia

phosphoinositide 3-kinase regulatory subunit 4 [Homo sapiens]

(Kts.mys  Fytiiniblogista  rakenne)

NCBI Reference Sequence: NP_055417.1
Identical Proteins FASTA Graphics

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LOCUS       NP_055417               1358 aa            linear   PRI 01-APR-2018
DEFINITION  phosphoinositide 3-kinase regulatory subunit 4 [Homo sapiens].
AC
ORIGIN      
        1 mgnqlagiap sqilsvesyf sdihdfeydk slgstrffkv arakhreglv vvkvfaiqdp
       61 tlpltsykqe leelkirlns aqnclpfqka sekasekaam lfrqyvrdnl ydristrpfl
      121 nniekrwiaf qiltavdqah ksgvrhgdik tenvmvtswn wvlltdfasf kptylpednp
      181 adfnyffdts rrrtcyiape rfvdggmfat eleymrdpst plvdlnsnqr trgelkramd
      241 ifsagcviae lftegvplfd lsqllayrng hffpeqvlnk iedhsirelv tqmihrepdk
      301 rleaedylkq qrgnafpeif ytflqpymaq faketflsad erilvirkdl gniihnlcgh
      361 dlpekaegep kenglvilvs vitsclqtlk ycdsklaale lilhlaprls veilldritp
      421 yllhfsndsv prvraealrt ltkvlalvke vprndiniyp eyilpgiahl aqddativrl
      481 ayaenialla etalrflelv qlknlnmend pnneeidevt hpngnydtel qalhemvqqk
      541 vvtllsdpen ivkqtlmeng itrlcvffgr qkandvllsh mitflndknd whlrgaffds
      601 ivgvaayvgw qsssilkpll qqglsdaeef vivkalyalt cmcqlgllqk phvyefasdi
      661 apflchpnlw irygavgfit vvarqistad vycklmpyld pyitqpiiqi erklvllsvl
      721 kepvsrsifd yalrskdits lfrhlhmrqk krngslpdcp ppedpaiaql lkkllsqgmt
      781 eeeedkllal kdfmmksnka kanivdqshl hdssqkgvid laalgitgrq vdlvktkqep
      841 ddkrarkhvk qdsnvneewk smfgsldppn mpqalpkgsd qeviqtgkpp rsessagicv
      901 plstssqvpe vttvqnkkpv ipvlsstilp styqirittc ktelqqliqq kreqcnaeri
      961 akqmmenaew eskppppgwr pkgllvahlh ehksavnrir vsdehslfat csndgtvkiw
     1021 nsqkmegktt ttrsiltysr iggrvktltf cqgshylaia sdngavqllg ieasklpksp
     1081 kihplqsril dqkedgcvvd mhhfnsgaqs vlayatvngs lvgwdlrsss nawtlkhdlk
     1141 sglitsfavd ihqcwlcigt ssgtmacwdm rfqlpisshc hpsrarirrl smhplyqswv
     1201 iaavqgnnev smwdmetgdr rftlwassap plselqpsph svhgiycspa dgnpilltag
     1261 sdmkirfwdl aypersyvva gstsspsvsy yrkiiegtev vqeiqnkqkv gpsddtprrg
     1321 peslpvghhd iitdvatfqt tqgfivtasr dgivkvwk
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