ESCRT-1, VPS37B (Kr.12q24.31) ( IST1 interaction region)

ESCRT-I, VPS37B 

One of  VPS37  four isoforms ,
 Interaction region with IST1(OLC1), which  is ESCRT III associated  factor. .
VPS37B has an interaction  with  adaptor ALG-2 stronger than TSG101 has
 in the  ternary complex ESCRT-1/ALIX-/ALG2.

https://www.ncbi.nlm.nih.gov/gene/79720

Preferred Names

vacuolar protein sorting-associated protein 37B

Names

ESCRT-I complex subunit VPS37B

hVps37B

vacuolar protein sorting 37 homolog B

vacuolar protein sorting 37B

Conservative domain

pfam07200
Location:11 → 155

Mod_r; Modifier of rudimentary (Mod(r)) protein

Peptide sequence:

(huom. kaksi LXXLL motiivia ja C-terminaalinen split P,  interaktiokohta  IST1 eli OLC1 kanssa) 

            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     Protein         1..285
                     /product="vacuolar protein sorting-associated protein 37B"
                     /note="hVps37B; ESCRT-I complex subunit VPS37B; vacuolar
                     protein sorting 37B; vacuolar protein sorting 37 homolog
                     B"
                     /calculated_mol_wt=31176
     Region          11..155
                     /region_name="Mod_r"
                     /note="Modifier of rudimentary (Mod(r)) protein;
                     pfam07200"
                     /db_xref="CDD:311259"
     Region          50..170
                     /region_name="Interaction with IST1.
                     {ECO:0000269|PubMed:19129479}"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H9H4.1)"
     Site            218
                     /site_type="methylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Omega-N-methylarginine.
                     {ECO:0000244|PubMed:24129315}; propagated from
                     UniProtKB/Swiss-Prot (Q9H9H4.1)"
     CDS             1..285
                     /gene="VPS37B"
                     /coded_by="NM_024667.2:104..961"
                     /db_xref="CCDS:CCDS9239.1"
                     /db_xref="GeneID:79720"
                     /db_xref="HGNC:HGNC:25754"
                     /db_xref="MIM:610037"
ORIGIN      
        1 magagsearf aglslvqlne lledegqlte mvqkmeetqn vqlnkemtla snrslaegnl
       61 lyqpqldtlk arltqkyqel qvlfeayqik ktkldrqsss asletllall qaegakieed
      121 tenmaekfld gelpldsfid vyqskrklah mrrvkieklq emvlkgqrlp qalaplpprl
      181 pelaptaplp ypapeasgpp avaprrippp pppvpagrla tpftaamssg qavpypglqc
      241 pplpprvglp tqqgfssqfv spyppplpqr ppprlpphqp gfilq
//

Related articles in PubMed

1. The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation. Bache KG, et al. Mol Biol Cell, 2004 Sep. PMID 15240819, Free PMC Article
2. The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex. Wunderley L, et al. J Cell Sci, 2014 Feb 1. PMID 24284069, Free PMC Article ESCRT-I is essential for the multivesicular body (MVB) sorting of ubiquitylated cargo such as epidermal growth factor receptor,(EGFR) as well as for several cellular functions, such as cell division and retroviral budding. ESCRT-I has four subunits; TSG101, VPS28, VPS37 and MVB12. There are several members of VPS37 and MVB12 families in mammalian cells, and their differential incorporation into ESCRT-I could provide function-specific variants of the complex. However, it remains unclear whether these different forms of VPS37 and MVB12 combine randomly or generate selective pairi ESCRT-I,ngs within ESCRT-I, and what the mechanistic basis for such pairing would be. Here, we show that the incorporation of two MVB12 members, UBAP1 and MVB12A, into ESCRT-I is highly selective with respect to their VPS37 partners.
3. Ubiquitin conjugation to Gag is essential for ESCRT-mediated HIV-1 budding. Sette P, et al. Retrovirology, 2013 Jul 29. PMID 23895345, Free PMC Article
4. In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters. Carlson LA, et al. Proc Natl Acad Sci U S A, 2012 Oct 16. PMID 23027949, Free PMC Article
5. VPS37 isoforms differentially modulate the ternary complex formation of ALIX, ALG-2, and ESCRT-I. Okumura M, et al. Biosci Biotechnol Biochem, 2013. PMID 23924735 Abstract The endosomal sorting complex required for transport (ESCRT) system comprises a series of protein complexes that play essential roles in multivesicular body (MVB) sorting of ubiquitylated membrane proteins, enveloped RNA virus budding, and cytokinesis in mammalian cells. The complex, named ESCRT-I, consists of four subunits (TSG101, VPS28, VPS37, and MVB12).
   There are four VPS37 isoforms. We have reported that ALIX (an ALG-2-interacting protein and accessory protein in the ESCRT system) is physically linked with TSG101 by ALG-2 in a Ca²⁺-dependent manner, but the role of ALG-2 as an adaptor protein for the ESCRT-I complex remains unknown. To characterize this adaptor function, initially we investigated the binding of ALG-2 to ESCRT-I complexes containing each one of the four different VPS37 isoforms by two approaches: ..VPS37B and VPS37C appeared to interact with ALG-2 in a stronger manner than TSG101 does. The results of in vitro binding assays using purified recombinant proteins indicated that ALG-2 functions as a Ca²⁺-dependent adaptor protein that bridges ALIX and ESCRT-I to form a ternary complex, ESCRT-I/ALIX/ALG-2.

See all (36) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. VPS37B and appeared to interact with ALG-2 in a stronger manner than TSG101 does.
2. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)