(Tomonori Kimura) TRIM proteiinit ja täsmennetty autofagia . WIPI:n paikka kuvassa

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http://jcs.biologists.org/content/joces/early/2016/02/11/jcs.163758.full.pdf

Precision autophagy directed by receptor regulators–emerging examples within the TRIM family

• Tomonori Kimura,  Michael Mandell and Vojo Dereti 

• Hyödyllinen yleiskatsaus  samalla TRIM-proteiinien funktioista. Trim-proteiineja ihmisessä 82  tämän artikkelin mukaan 2016. Tämä on talletettu niille, joita  TRIM- proteiinien  yleiskatsaus  kiinnostaa. COMMENTARY  Tomonori Kimura*, Michael Mandell and Vojo Deretic 

• *ABSTRACT

•  Selective autophagy entails cooperation between target recognitionand assembly of the autophagic apparatus. Target recognition is conducted by receptors that often recognize tags, such as ubiquitinand galectins, although examples of selective autophagy independent of these tags are emerging. It is less known how receptors cooperate with the upstream autophagic regulators, beyond the well-characterized association of receptors with Atg8 or its homologs, such as LC3B (encoded by MAP1LC3B), on autophagic membranes. The molecular details of the emerging role in autophagy of the family of proteins called TRIMs shed light on the coordinatio nbetween cargo recognition and the assembly and activation of the principal autophagy regulators. In their autophagy roles, TRIMs act both as receptors and as platforms (‘receptor regulators’) for the assembly of the core autophagy regulators, such as ULK1 and Beclin 1 in their activated state. As autophagic receptors, TRIMs can directly recognize endogenous or exogenous targets, obviating a need for intermediary autophagic tags, such as ubiquitin and galectins. The receptor and regulatory features embodied within the same entity allow TRIMs to govern cargo degradation in a highly exact process termed‘precision autophagy’.
•  KEY WORDS: Receptor regulators, TRIMs, Autophagy

•  Introduction 

• Autophagy is a set of diverse processes, often classified as macro-autophagy, micro-autophagy and chaperone-mediated autophagy(CMA),which recognize and deliver endogenous cellular constituents orexogenous cargo to lysosomes for degradation (Mizushima et al.,2011).
•  Autophagy can occur in bulk as a response to starvation,whereby portions of the cytoplasm are auto-digested to meet the heightened biogenesis and energy needs of the cell (Rabinowitz andWhite, 2010; Galluzzi et al., 2014). 
• Autophagy plays equally important roles in homeostasis, acting as a quality and quantity control process by removing protein aggregates (Rubinsztein et al.,2012; Birgisdottir et al., 2013) and defunct or surplus organelles (Maejima et al., 2013; Randow and Youle, 2014), as well as invading microbes and endogenous and exogenous inflammatory agonists (Deretic et al., 2015). The degradation targets for autophagy are not limited to cargo of the cytoplasmic origin, as autophagy can also target nuclear components (Dou et al., 2015). 
• The selectivity of autophagy (Fig. 1) has been a topic of continuing interest; it has been established that a number of receptors can guide autophagic machinery to targets, which are often earmarked for autophagy with ubiquitin or galectins tags (Randow and Youle,2014; Stolz et al., 2014).
•  A morphologically emblematic event during autophagy is the formation in the cytoplasm of the membranous organelles termed autophagosomes; these are the executors of macro-autophagy (hereafter referred to as autophagy) (Mizushima et al., 2011). 
• Autophagosomes are decorated in yeast with Atg8 and in mammals with LC3B [one of six mammalian (m)Atg8 paralogs–LC3A,LC3B and LC3C (encoded byMAP1LC3A,MAP1LC3BandMAP1LC3C, respectively) and GABARAP, GABARAPL1 andGABARAPL2 (Mizushima et al., 2011; Weidberg et al., 2011)].mAtg8 proteins are conjugated at their C-terminus to a lipid, phosphatidylethanolamine (PtdEtn) (PE), which in turn mediates association with the autophagic membrane (Kabeya et al., 2000). 
• Many other parts of the core autophagy machinery are well-conserved from yeast to humans (Mizushima et al., 2011). In mammals, the key autophagy factors (ATG) include ULK1 (one of four mammalian paralogs of Atg1) (Chan et al., 2007; Kundu andThompson, 2008) and Beclin 1 (a paralog of yeast Atg6) (Lianget al., 1999). These principal autophagy regulators are controlled by upstream serine and threonine (Ser/Thr) protein kinases 5′-AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) (Egan et al., 2011; Kim et al., 2011, 2013;Russell et al., 2013), as well as E3 ligases and ubiquitylation (Nazioet al., 2013).
• Lipid kinases, most notably the class III  phosphatidylinositol 3-kinase (PI3K) VPS34 (encoded byPIK3C3; which forms a complex with Beclin 1), co-activate autophagy by producing phosphatidylinositol 3-phosphate[PtdIns(3)P], a step that commits them to participating in autophagosome formation (Petiot et al., 2000); 
• however, the roles of other mono-phosphoinositides such as phosphatidylinositol 5-phosphate (PI5P)  have recently been suggested (Vicinanza et al., 2015). 
• The protein kinases (ULK1), lipid kinases (Beclin-1–VPS34) and the LC3-conjugation system are all physically connected in their role in controlling autophagy. 
•  First, PtdIns(3)P (PI3P) is recognized byWD repeat domain phosphoinositide-interacting protein 2 (WIPI2, the mammalian Atg18), which interacts with ATG16L1 and forms part of the LC3–PtdEtn conjugation system (Dooley et al., 2014).
•  Second, ATG16L1 associates with FIP200 (also known asRB1CC1), a component of the ULK1 complex system (Fujitaet al., 2013; Gammoh et al., 2013; Nishimura et al., 2013; Dooleyet al., 2014).
•  Autophagy is further coupled to the lysosomal systems.This occurs through the effects on autophagy of members of the key regulator of lysosomal biogenesis‘microphthalmia/transcriptionfactor E’(MiT/TFE) family (including transcription factor EB;TFEB) (Settembre et al., 2011; Perera et al., 2015) and through SNAREs (Itakura et al., 2012), which enable the maturation of autophagosomes into the autolysosome. 
• Thus, all parts of the autophagy system are interconnected, thereby enabling spatial and temporal regulation of autophagy.The homing of the autophagy machinery to intended targets through receptors is an area of intense study (Birgisdottir et al.,2013; Rogov et al., 2014; Khaminets et al., 2015). Frequently, targets exhibit ubiquitin or galectin marks, although this does not appear to be an absolute requirement (Khaminets et al., 2015). Furthermore, the number of proteinaceous, membranous and complex targets for selective autophagy is rapidly increasing. This raises an important question: are the number of receptors and the principles that define them described thus far sufficient to explain all types of selective autophagy? In this Commentary, we will review recent studies on one such family of factors, TRIMs, which appear to be different from conventional receptors because they perform two separate but interlinked duties, by acting both as autophagic receptors and autophagy regulators. Importantly, TRIMs have been shown in several cases to directly recognize their cognate targets that are destined for autophagy without the need for intermediary tags such as ubiquitin and galectins. These functionalities are the basis for a process we have termed‘precision autophagy’(Kimura et al.,2015)

•  Sequestosome-1-like receptors and cargo recognition mediated by tags 

• As noted above, the cargo entering the autophagosomes is often modified with tags, such as ubiquitin, phosphorylated ubiquitin and galectins (Koyano et al., 2014; Randow and Youle, 2014;Khaminets et al., 2015). These tags are then recognized by sequestosome-1-like receptors (SLRs) named so (Birgisdottiret al., 2013; Deretic et al., 2013) after the founding member of this receptor family, sequestosome 1 (commonly known as p62)(Lamark et al., 2009). 
• SLRs also include other receptors, such as neighbor of BRCA1 gene 1 (NBR1), Ca2+-binding and coiled-coildomain 2 (CALCOCO2, commonly known as NDP52), tax-bindingprotein 1 (TAXBP1) and optineurin (Bjørkøy et al., 2005; Kirkinet al., 2009; Wild et al., 2011; Newman et al., 2012; Thurston et al.,2012; Deretic et al., 2013; Lazarou et al., 2015). SLRs bind both to mAtg8 proteins through LC3-interacting motifs (LIR) and to ubiquitin through a variety of ubiquitin-binding domains (e.g.UBAN, UBA, UBZ) (Khaminets et al., 2015). Some SLRs bind to galectin tags (Thurston et al., 2012). Besides the above-mentioned group of mammalian proteins, additional receptors fit the SLR criteria. For instance, RPN10, an Arabidopsis receptor that mediates autophagic degradation of proteasome, binds to ubiquitin tags and Atg8s through its ubiquitin-interacting motif (UIM) domains(Marshall et al., 2015). Furthermore, Toll-interacting protein (TOLLIP) in mammals and Cue5 in yeast fit SLR criteria; Cue5 directly binds both to ubiquitin and to Atg8, whereas TOLLIP directly binds to ubiquitin and, in cellular extracts, to LC3 (Lu et al.,2014). 

• The TRIM protein family 

• TRIMs were first recognized as a family (Reymond et al., 2001) based on their common tri-partite domain organization, typically comprising an N-terminal RING domain, one or two B-boxdomains and a coiled-coil domain(RBBCC) , although they also have additional features, including a variety of C-terminal domains, inclusive of SPRY domains (Fig. 2A,B; Table S1). TRIMs are conserved in metazoans, and the number of genes encoding them escalates in higher organisms (Sardiello et al., 2008; Boudinot et al.,2011);
•  82 TRIM genes (excluding the pseudogenes) have been identified in humans (Table S1).TRIMs are involved in a variety of seemingly disparate cellular processes (Table S1); these include cell survival and death (Grignani et al., 1993), cell cycle and differentiation (Wang et al.,1998), metabolic states (Skurat et al., 2002), cell membrane repair(Cai et al., 2009), synaptic vesicle exocytosis (Li et al., 2001),senescence (Pearson et al., 2000), stem cell pluripotency (Sato et al.,2012) and erythrocyte differentiation (Blaybel et al., 2008; Bardeet al., 2013), as well as the control of viral, bacterial and fungal infections (Nisole et al., 2005; Rakebrandt et al., 2014; Cao et al.,2015). These and additional roles are linked to a variety of health conditions. For instance, TRIMs have been implicated in autoimmune and inflammatory disorders (Jefferies et al., 2011;Kawai and Akira, 2011; Kimura et al., 2015) that are associated with genetic polymorphisms (French FMF Consortium, 1997; The International FMF Consortium Guido, 1997), as well as in insulin resistance (Karlberg et al., 2005; Song et al., 2013), urate metabolism (Köttgen et al., 2013), cancer (Hatakeyama, 2011), asthma (Shin et al., 2011; Collison et al., 2013), and neurodegenerative and neuromuscular diseases (Leonhardt et al.,1994; Khan et al., 2014). Among the best-studied roles for TRIMs is their role in cell-autonomous antiviral defense, a trend partially driven by intense research focusing on human immunodeficiency virus (HIV) (Stremlau et al., 2004; Nisole et al., 2005; Pertel et al.,2011; Versteeg et al., 2013). 

• TRIMs function as autophagic‘receptor regulators’

•  A growing number of studies indicate that several TRIMs are linked to autophagy (Fig. 2C; Table S1) (Lipinski et al., 2010; Niida et al.,2010; Perera et al., 2011; McKnight et al., 2012; Tomar et al., 2012;Barde et al., 2013; Pizon et al., 2013; Yang et al., 2013; Khan et al.,2014; Mandell et al., 2014; Choi et al., 2015; Kimura et al., 2015). 
• TRIMs act as autophagy receptor regulators (meaning that they act as both receptors and platforms for assembly of autophagosome machinery) by two means.
•  Firstly, TRIMs recognize their targets (Mandell et al., 2014; Kimura et al., 2015) by finding their cognate cargo (e.g. viral core, inflammasome components) through direct protein–protein binding without a need for ubiquitin or galectin intermediates (Figs 1 and 3A).
•  Secondly, the same TRIMs that function as receptors then also act as platforms for the assembly of the core regulators of autophagy, such as ULK1, Beclin1, ATG16L1(Mandell et al., 2014; Kimura et al., 2015) (Table S1). These two features distinguish TRIMs from SLRs (Table S2a) and from other unique receptors (Table S2b), and allow the execution of a highly selective form of autophagy, termed precision autophagy (Kimuraet al., 2015). 

• TRIMs as receptors

•  TRIMs recognize both endogenous and exogenous cargo for autophagy (Fig. 2C). For instance, TRIM20 (also known as MEFV)and TRIM21 have been shown to act as receptors for specific endogenous autophagy targets (Kimura et al., 2015). TRIM20 recognizes the inflammasome components pro-caspase 1, and NLR-family pyrin-domain-containing 1 and 3 proteins (NLRP1 andNLRP3), and presents them for autophagic degradation (Fig. 2C).TRIM21 targets the activated (dimerized) form of interferonregulatory factor 3 (IRF-3), a key transcriptional regulator of type-I interferon (IFN) genes, to mediate autophagy. Both TRIM20 andTRIM21 bind to their targets through their C-terminal SPRYdomains.RhesusTRIM5 recognizes an exogenous target–it bindsto the HIV-1 capsid within the incoming viral core directly throughits C-terminal SPRY domain (Stremlau et al., 2006; Ganser-Pornillos et al., 2011). This delivers the capsid protein p24 (CA) forTRIM5-directed autophagic degradation (Mandell et al., 2014),contributing to anti-retroviral modalities that are mediated byTRIM5 (Pertel et al., 2011).The domains present at the C-terminus in the different TRIMs show a considerable variety (Fig. 2A,B; Table S1), suggesting thatTRIMs recognize diverse cargo. Domains at the C-termini include Fibronectin type-III repeats (FN3) for DNA or heparin binding, PHDfingers for histone binding, BROMO for acetylated lysine residue binding, FIL for actin binding, COS for microtubule binding, and MATH as well as NHL domains, in addition to the above mentioned SPRY (also known as PRY/SPRY) domain used in protein binding (Fig. 2B) (Reymond et al., 2001; Ozato et al., 2008; Sardiello et al.,2008). Moreover, even a single SPRY domain within the same TRIM can interact with many partners and potential substrates (Papin et al., 2007). In addition, the ability of TRIMs to hetero-oligomerize (Cao et al., 1997; Reymond et al., 2001; Bell et al.,2012; Kimura et al., 2015) might allow for a cooperative binding to diverse cargos, targeting them for degradation by autophagy. 

• TRIM receptors interact with mAtg8s 

• A common feature of conventional receptors for selective autophagy (Birgisdottir et al., 2013; Randow and Youle, 2014;Rogov et al., 2014; Stolz et al., 2014), such as SLRs, is that they bindto mAtg8s (e.g. LC3B or LC3C), which links them to autophagosomal membranes. TRIM5, TRIM20 and TRIM21(Mandell et al., 2014; Kimura et al., 2015) bind to mAtg8s(Fig. 2A). Other TRIMs interact with mAtg8s (Table S1)in vitro,asshown for TRIM16, TRIM17, TRIM22, TRIM49 and TRIM55 (Mandell et al., 2014). Colocalization between mAtg8s and theTRIMs listed above in the cytoplasm (Niida et al., 2010; Pizon et al.,2013; Mandell et al., 2014; Kimura et al., 2015), as well as with TRIM63 (Khan et al., 2014), corroborate the biochemical findings. Potential binding to mAtg8 has also been documented for TRIM19 [also known as promyelocytic leukemia (PML) protein] andTRIM28 (Behrends et al., 2010; He et al., 2014). TRIM19 co-immunoprecipitates with LC3B and colocalizes with it in the nucleus (He et al., 2014); this is of interest given that LC3B shuttles between the nucleus and the cytosol upon deacetylation in association with autophagy activation (Huang et al., 2015) and plays a role in autophagic clearance of nuclear components (Douet al., 2015). The binding sites for mAtg8 (LIRs; Fig. 2A; Table S1) on several TRIMs have been mapped. TRIM5 has two LIRs, whereas TRIM20 has three LIRs (Mandell et al., 2014; Kimura et al., 2015). Only when all LIRs are mutated or deleted do TRIMs lose their ability to bind to mAtg8s (Mandell et al., 2014; Kimura et al., 2015). TheTRIMs show a preference for GABARAPs and LC3A, and do notbind to LC3B when tested in vitro. Nevertheless, LC3B can befound in larger protein complexes with TRIMs when precipitated from cells (Pizon et al., 2013; He et al., 2014; Mandell et al., 2014).Thus, it is likely that LC3B forms larger complexes with TRIMs,possibly indirectly and in a manner that is mediated by an intermediary protein (e.g. p62, discussed below) or following putative post-translational modifications.

•  Some TRIMs bind to SLRs

•  Certain TRIMs have the ability to bind to SLRs, such as p62(Fig. 3A); the known examples include TRIM5 (O’Connor et al.,2010), TRIM50 (Fusco et al., 2012), TRIM55 (Lange et al., 2005;Pizon et al., 2013; Mandell et al., 2014), TRIM13 (Tomar et al.,2012), TRIM17 and TRIM49 (Mandell et al., 2014), TRIM21(Kimura et al., 2015), as well as TRIM76 (Blandin et al., 2013).Furthermore, p62 can colocalize with TRIM63 in the cytoplasm(Khan et al., 2014) and with TRIM19 in the nucleus within thenuclear PML bodies (Pankiv et al., 2010), although this might not be restricted to the nucleus because TRIM19 functions in thecytoplasm as well (Lin et al., 2004; Dutrieux et al., 2015). The association with p62 might link TRIMs indirectly to LC3B (asdiscussed above); this is of significance for those TRIMs with no evidence of direct binding to LC3B. In contrast, TRIM16 andTRIM20 do not appear to associate with p62 (Mandell et al., 2014). Whether these TRIMs associate with other SLRs or do not interactwith SLRs is unknown. Similar to p62, some TRIMs can themselves be degraded by autophagy. For instance, TRIM50 (Fusco et al., 2012) and murineTRIM30, a homolog of human TRIM5, are degraded by autophagy (Choi et al., 2015). TRIM20, which mediates autophagic degradation of NLRP3, is degraded in autolysosomes but only in the presence of its target (Kimura et al., 2015).

•  TRIMs as autophagy regulators 

• TRIMs affect autophagy as a whole process (Lipinski et al., 2010;Perera et al., 2011; McKnight et al., 2012; Tomar et al., 2012; Bardeet al., 2013; Pizon et al., 2013; Khan et al., 2014; Pineda et al., 2015)and interact with ATG factors (Behrends et al., 2010; Yang et al.,2013; Mandell et al., 2014; Kimura et al., 2015). 
• Of the 82 humanTRIMs, 49 TRIMs have an effect on autophagy (Table S1). The firstscreens of TRIMs (Mandell et al., 2014) assessed the role of the entire TRIM family on autophagy that had been induced by inhibition of mTOR, a classic way of activating autophagosome formation. Using this approach and pp242 as an mTOR inhibitor, 32 TRIMs were identified as regulating autophagy and TRIM5 was among those hits (Mandell et al., 2014).
•  Another screen (Kimuraet al., 2015) that aimed to identify TRIMs affecting immunologically induced autophagy utilized IFN-γ, which is known from earlier studies to induce autophagy but with incompletely understood molecular mechanisms. These studies have revealed that 24 TRIMs (including TRIM8, TRIM20 andTRIM21) are required for optimal induction of autophagy through IFN-γ (Kimura et al., 2015). 
•  Several genome-wide screens, albeit not aimed at studying TRIMs, indicate that a number of additional TRIMs (TRIM29, TRIM51 and TRIM69) modulate autophagy (Lipinski et al., 2010; McKnight et al., 2012).
•  Furthermore,TRIM13, TRIM28, TRIM55, TRIM56 and TRIM63 have been reported in separate studies to affect autophagy (Tomar et al., 2012;Barde et al., 2013; Pizon et al., 2013; Yang et al., 2013; Khan et al.,2014; Kimura et al., 2015; Pineda et al., 2015).
•  Two recent studies show that TRIMs directly assemble the cardinal autophagy regulators ULK1 and Beclin 1 into a proteincomplex that we have called the TRIMosome (Fig. 3B). Within aTRIMosome, individual TRIMs (e.g. TRIM5, TRIM6, TRIM17,TRIM20, TRIM21, TRIM22 and TRIM49) form protein complexes with both ULK1 and Beclin 1 (Mandell et al., 2014; Kimura et al.,2015). 
• Induction of autophagy depends on a phosphorylation cascade that includes activation of ULK1 by AMPK-mediated phosphorylation at several sites, including Ser317 and Ser555(Egan et al., 2011; Kim et al., 2011). 
• TRIMosomes contain detectable amounts of AMPK (Kimura et al., 2015) and are enriched for the activated form of ULK1–i.e. phosphorylated by AMPK atSer317 (Mandell et al., 2014; Kimura et al., 2015) and Ser555 (Kimura et al., 2015).  
• Beclin 1 within the TRIMosome complexes is phosphorylated at Ser15 (Mandell et al., 2014), an indication of its activation by ULK1 (Russell et al., 2013), and at Ser91 and Ser94, indicative of activation by AMPK (Kim et al., 2013). These molecular events are reminiscent of the assembly of activated ULK1 and Beclin 1, which are anchored by immunity-related GTPase M(IRGM) (Chauhan et al., 2015), another type of platform for the assembly of autophagic machinery. 
• TRIM8, identified in a screen for IFN-γ-inducible autophagyregulators (Kimura et al., 2015), ubiquitylates and activates TGFβ-activated kinase 1 (TAK1; also known as NR2C2) (Li et al., 2011),which in turn activates AMPK (Xie et al., 2006), and so links to key roles of AMPK described above. 
• Several TRIMs (e.g. TRIM13 and TRIM5) show spatial juxtaposition to double FYVE-containing protein 1 (DFCP1) (Tomar et al., 2012; Mandell et al., 2014), a marker for the omegasome, which is believed to be the cradle for autophagosome formation from the endoplasmic reticulum (ER)(Axe et al., 2008). 
• Other autophagy factors, FIP200 (an ULK1interactor), VPS34, ATG14L and UVRAG (Beclin-1-interactingproteins), WIPI2 [a PtdIns(3)P-binding protein interacting with ATG16L1], ATG16L1, ATG5 and ATG12 (an‘E3 ligase for mAtg8 lipidation), and activating molecule in BECN1-regulated autophagyprotein 1 (AMBRA1) have been detected in complexes with TRIMs(Behrends et al., 2010; Yang et al., 2013; Mandell et al., 2014;Kimura et al., 2015).
• TRIM5 activates this complex by promoting dissociation of the negative regulators Bcl-2 and TAB2 (Wei et al.,2008; Criollo et al., 2011; Takaesu et al., 2012) from Beclin 1(Mandell et al., 2014); this is likely to occur in conjunction with the Beclin 1 phosphorylation events described above.TRIM-dependent regulation can both accelerate and decelerate autophagy. 
• TRIM17, a negative regulator of pp242-induced autophagy, sequesters the autophagy machinery away from bulk autophagy (Mandell et al., 2014). 
• Furthermore, even a specificTRIM protein might have positive and negative effects on autophagy, as in the case of TRIM28 (Barde et al., 2013; Yanget al., 2013; Mandell et al., 2014; Pineda et al., 2015). For its positive effect, TRIM28 activates VPS34 by modulating binding to Beclin 1 (Yang et al., 2013). For its negative effect, TRIM28, as an E3 ligase, has been reported to mediate proteasomal degradation of AMPK, potentially downregulating autophagy (Pineda et al., 2015). 
• Splice variants of TRIM55 have different activities in the proteasome and autophagy systems during muscle cell differentiation (Pizon et al., 2013), increasing one or the other. 

• Precision autophagy 

• The definition of precision autophagy is based on the two fold principle that the same entity (e.g. a TRIM) recognizes directly itscognate autophagic cargo without a need for tags such as ubiquitin or galectins, and at the same time mediating the assembly of core ATG factors into autophagy-activating complexes (Kimura et al., 2015). An important question arises as to whether the previously described conventional receptors could eventually fit into the broader definition of precision autophagy (Birgisdottir et al., 2013; Randow and Youle, 2014; Rogov et al., 2014; Stolz et al., 2014).These receptors, such as p62, associate with other factors, including autophagy-linked FYVE protein (ALFY; also known as WDFY3)(Clausen et al., 2010), which in turn binds to ATG5, ATG12 andATG16L1 (Filimonenko et al., 2010). 
• ULK1 and p62 colocalize on the ER in the early stages of autophagy initiation (Itakura andMizushima, 2010), and p62 binds to ULK1 and ULK2 (Pridgeonet al., 2003; Ro et al., 2014). NDP52 interacts with FIP200 (Wanget al., 2011), and recent studies with optineurin and NDP52 hint that these proteins affect the distribution of autophagy regulators such as ULK1, WIPI1 and DFCP1 (Lazarou et al., 2015). These observations suggest that SLRs, either alone or in combination with other platforms, could act in precision autophagy akin to TRIMs, but more work is needed to establish such relationships.There are also other platform-forming complexes that, similar to TRIMs, assemble ATG factors, such as the exocyst complex (Bodemann et al., 2011) and IRGM (Chauhan et al., 2015);however, cargo receptors within such complexes have not been defined. Thus, a broader definition of precision autophagy might emerge that encompasses additional new or previously studied receptors and molecular assemblies

• Other autophagy receptors 

• Beyond the receptor classes of TRIMs (Table S1) and SLRs(Table S2a), unique autophagy receptors have also been described (Table S2b) that participate in specific forms of autophagy. Atg32 isa receptor for mitophagy in yeast (Kanki et al., 2009; Okamotoet al., 2009) and its equivalent, Bcl2-L-13, has been identified in mammals (Murakawa et al., 2015). During reticulocyte development, Nix (also known as BNIP3L) appears to be the receptor for mitophagy (Sandoval et al., 2008; Schwarten et al.,2009; Novak et al., 2010).
•  FUNDC1 (Liu et al., 2012) and BNIP3(Zhang et al., 2008; Zhu et al., 2013) contribute to mitophagy during hypoxia in mammals. Nevertheless, there is evidence that subsets of SLRs (Lazarou et al., 2015; Matsumoto et al., 2015) playa role in mammalian mitophagy in conjunction with the PINK1–Parkin–phosphorylated-ubiquitin system (Koyano et al., 2014;Lazarou et al., 2015), which is activated in response to mitochondrial damage. 
• Of note, the mitochondria-specific phospholipid cardiolipin is directly recognized by LC3B (Chuet al., 2013), potentially short-circuiting or aiding receptor recognition. A further implication of lipid recognition in mitophagy comes from the contribution to this process of SMURF1 (Orvedahl et al., 2011)–a C2-domain-containing protein (indicative of a lipid recognition)–and IRGM, which binds to cardiolipin (Singh et al., 2010). How all of these receptors and factors connect with the upstream regulators of autophagy remains to be determined.
•  Other organelles are recognized by unique receptors in order to be targeted for autophagy. In yeast and Pichia, pexophagy occurs through the peroxisome-anchored receptors Atg30 and Atg36 (Farréet al., 2008; Motley et al., 2012); albeit pexophagy that is mediated by the SLR NBR1 has been described in mammalian cells (Deosaran et al., 2013). 
• ER-phagy is facilitated by membrane-associated receptors, such as FAM134B in mammals (Khaminetset al., 2015) and Atg40 (the functional counterpart of FAM134B inyeast); these function as receptors for autophagy of peripheral ER,whereas an additional receptor, Atg39 acts on peri-nuclear ER(Mochida et al., 2015).Various proteinaceous targets and complexes are recognized not through ubiquitin but through unique receptors. 
• NCOA4 has a receptor role in autophagy of ferritin (Dowdle et al., 2014; Manciaset al., 2014); NCOA4 binds to mAtg8 proteins in a manner similar to that of TRIMs with the exception of LC3B and recruits the large 450-kDa ferritin complex to the autolysosome (Dowdle et al., 2014;Mancias et al., 2014), thereby contributing to iron homeostasis.
•  STBD1, which binds to GABARAP and GABARAPL1, and glycogen synthase, which binds to Atg8 inDrosophila, could be receptors that recognise glycogen for autophagic degradation (Jianget al., 2011; Zirin et al., 2013). 

• Role of TRIM-directed precision autophagy in disease 

• TRIMs have a role in infectious, autoimmune and neoplastic diseases (Ozato et al., 2008; Hatakeyama, 2011; Jefferies et al.,2011; Kawai and Akira, 2011). In the context of inflammatory etiology of disease, autophagy can suppress inflammasome output either indirectly (Saitoh et al., 2008; Schroder and Tschopp, 2010;Nakahira et al., 2011; Zhou et al., 2011), or directly through selective autophagy of assembled inflammasomes (Shi et al., 2012) or inflammasome components, such as that mediated by TRIM20 (Kimura et al., 2015). 
• TRIM20, also known as PYRIN, is encode dby the MEFVgene, which is the risk locus for familial Mediterranean fever (FMF). TRIM20 has 308 FMF-associated variants (http://fmf.igh.cnrs.fr/ISSAID/infevers/), and these polymorphisms commonly occur in its PRY/SPRY domain (Masters et al., 2009). TRIM20 variants with mutations in thePRY/SPRY domain show deficiency in the autophagic degradation of NLRP3 (Kimura et al., 2015). Additional FMF-associated polymorphisms fall within the TRIM20 LIR motifs (E403K in thefirst LIR motif, Y471X and E474K in the second LIR motif). Of note, TRIM20 expression is strongly induced by IFN-γ(Carthagenaet al., 2009; Chae et al., 2011). Thus, TRIM expression is regulated,which in turn determines the outcome–i.e. the IFN-γ–TRIM20–autophagy axis might suppress excessive inflammasome activation,in keeping with the notion that inflammasome activation can beinhibited by IFN-γ(Chae et al., 2011). This capacity could becompromised in FMF owing to TRIM20 polymorphisms, whichreduces the autophagic degradation of inflammasome components. 
• One of the hallmarks of autoimmune diseases, such as systemiclupus erythematosus (SLE) and Sjögren syndrome, is the activationof type-I IFN (Banchereau and Pascual, 2006). The autophagic receptor regulator TRIM21 directs precision autophagy of activated (dimerized) IRF-3, thereby reducing activation of type-I IFN (Kimura et al., 2015). This is also reflected in the related ability of TRIM21 to degrade inhibitor of NF-κB kinase (IKKβ, encoded by IKBKB) through autophagy (Niida et al., 2010). TRIM21 also targets other factors that are responsible for type-I IFN activation, includingIRF-5, IRF-7 and IRF-8, for degradation through proteasomal or, thus far, unknown mechanisms (Higgs et al., 2008, 2010; Espinosaet al., 2009; Yoshimi et al., 2009; Young et al., 2011; Lazzari et al.,2014). 
• TRIMs are often described as autoantigens in autoimmunediseases and cancers, including TRIM21 and TRIM68 in SLE and Sjögren syndrome (Der et al., 1998; Billaut-Mulot et al., 2001), 
• TRIM33 in dermatomyositis and the associated paraneoplastic phenomena (Targoff et al., 2006; Fujimoto et al., 2012),
•  TRIM19 in primary biliary cirrhosis (Stinton et al., 2011), and
•  TRIM28 in coloncancer (Kijanka et al., 2010; Hector et al., 2012) and dermatomyositis (Satoh et al., 2012). 
• Furthermore, TRIMs as a protein family have been associated with the regulation of type-I IFN responses (Versteeg et al., 2013), in keeping with relationships described for TRIM21 above (Kimura et al., 2015).TRIMs play a role in defense against viral pathogens (Nisole et al.,2005), which at least in the case of TRIM5 involves precisionautophagy (Mandell et al., 2014). TheRhesusTRIM5 can execute precision autophagy of the HIV-1 capsid. In contrast, the weak affinity of human TRIM5 for the HIV-1 capsid precludes effective precision autophagy. As a consequence, RhesusTRIM5, but not human TRIM5, contributes to defense against HIV-1 through precision autophagy. Antimicrobial roles of TRIMs that are mediated through autophagy might extend to a defense against other intracellular pathogens; this includes bacteria (Rakebrandt et al.,2014) and possibly fungi. 
• For instance, the antifungal activity ofTRIM62 that is conveyed by CARD9, a mediator of innate immunity signals, which although not yet formally linked to autophagy (Caoet al., 2015), is known to play a role in autophagy through the interactions it has with RUBICON (encoded b yKIAA0226)(Yanget al., 2012). 
• A number of mutations and polymorphisms in TRIMs are known to be associated with cancer, neurodegeneration, neuro-muscular diseases, immune disease (e.g. FMF as described above), infections (as in the case of HIV-1 susceptibility), metabolic traits, neurodegeneration, psychiatric disorders, heart disease and aging processes (Table S1).
•  Several TRIMs are also involved in oncogenesis (Table S1). A classic example of this is TRIM19,which is fused with retinoic acid receptorα(RARα) in acute PMLdisease (de Thé et al., 1991; Kakizuka et al., 1991). Furthermore, (sivu 6)
•  fusions between TRIM24 and B-Raf are found in liver cancer (LeDouarin et al., 1995), between TRIM27 and RET in papillary thyroid cancer (Hasegawa et al., 1996), and of TRIM4 with B-Raf inlung cancer (Zheng et al., 2014) or with MET in melanoma (Yehet al., 2015). Among these, the TRIM19–RARα fusion protein is already known to be degraded by autophagy (Isakson et al., 2010;Wang et al., 2014), whereas clearance of TRIM19–RARαis a therapeutic target in acute PML disease (Nasr et al., 2008). Thus,precision autophagy of TRIM19–RARαand other oncogenic fusions could be beneficial in cancer treatment (Fig. 2C). 
• A recent sequencing of tumor tissues has revealed that TRIM23 is a previously unappreciated gene involved in cancer progression(Lawrence et al., 2014). 
• TRIMs are also known to regulate tumorsuppressor proteins such as p53 (encoded byTP53) (Hatakeyama,2011).
•  Indeed, TRIM24 (Allton et al., 2009), TRIM39 (Zhang et al.,2012a,b) and TRIM59 (Zhou et al., 2014) help to degrade p53. 
• Inaddition, TRIM8, TRIM13, TRIM19, TRIM21 and TRIM25 also affect p53 stability (Bernardi et al., 2004; Joo et al., 2011;Caratozzolo et al., 2012; Reddy et al., 2014; Zhang et al., 2015). 
• TRIM28, TRIM29 and TRIM32 antagonize p53 function (Wanget al., 2005; Yuan et al., 2010; Liu et al., 2014). 
• In turn, TRIMs and p53 affect each other transcriptionally. TRIM3 increases the level of p53 (Cheung et al., 2010), and the expression of TRIM22 is increased by p53 (Obad et al., 2004), whereas that of TRIM9 is increased by mutated p53 (Okaichi et al., 2013). 
• The relationship between TRIMs and p53 fit with the dual role of p53 in autophagy (Tasdemir et al., 2008; Tavernarakis et al., 2008).A recent study has linked TRIM-directed autophagy with certain types of neurodegeneration (Khan et al., 2014). 
• TRIM63 mediates autophagy to promote the turnover of nicotinic acetylcholinereceptor in muscles to remodel neuromuscular junctions (Fig. 2C)(Kim et al., 2014). Thus, TRIMs and precision autophagy could play a wider role in neurodegenerative diseases.

•  Conclusion and future perspectives 

• The role of the TRIM proteins as autophagic receptors widens our understanding of the repertoire, versatility and capacity for selective autophagy in mammalian cells. In their role as assembly platforms,TRIMs provide a new paradigm for the organization, localization and homing of the autophagic apparatus in mammalian cells. The TRIM family has numerous members (82 members in humans), suggesting that they account for the regulation of a significant portion of selective autophagy. Moreover, the TRIM family has expanded over the course of evolution, suggesting that this family co-evolves with the expanding need for clearance of more complexor emerging targets. Given the breadth of the role of TRIMs in various diseases (Table S1), it will be important to explore precision autophagy–in addition to bulk autophagy–as a therapeutic target.The integration of TRIMs with autophagy is
•  multi-tiered. When TRIMs act as selective autophagy receptors, they also assemble the principal core autophagy regulators. This represents a key feature of precision autophagy. The physical‘home’or an embodiment of this dual role as both receptors and regulators is the TRIMosome (Fig. 3B), which furthermore might act as a mammalian equivalent of the phagophore-assembly site (PAS; also known as pre-autophagosomal structure) for precision autophagy. The TRIMosome proteinaceous organelle, or apparatus, initiates and executes autophagy in coordination with the recognition of its intended substrate.The above concepts and relationships raise questions, such as how many TRIMs act as autophagic receptors and what are theirs pecific targets? Do different TRIMs cooperate through their known  heterotypic interactions in clearance of more complex (multivalent)targets? How is the autophagic role of TRIMs integrated with the other functions of TRIMs, including regulation of gene expression and pro-inflammatory signaling? What is the interplay between the E3 ligase activity of TRIMs and precision (or other forms of)autophagy? Do TRIMs regulate autophagic maturation in addition to the initiation of the process? Can TRIMs also negatively regulate autophagy through competition or by direct inhibitory activities,and does this reflect the need for a homeostatic‘off switch’in orderto limit autophagy after it has been initiated? Finally, are there receptors other than TRIMs that fit the concept of receptor regulators and precision autophagy? Answering these questionsand gaining a further understanding of the TRIMs promises to enlighten us about the specifics of how autophagy is deployed inmammalian cells. 
• Acknowledgement We thank Drs Terje Johansen and Ashish Jain for important collaborativecontributions to TRIM studies. We thank Drs Beth Levine and Ivan Dikic for inputregarding the definition of precision autophagy