ESCRT-assosioitunut faktori, IST-1 ( Kr.16q22.2), OLC1

 IST_1
Tämä IST-1 säätelee VSP4:ää ja on välttämätön ESCART- koneistossa. 
https://www.ncbi.nlm.nih.gov/gene/9798

Preferred Names

IST1 homolog

Names

IST1, endosomal sorting complex required for transport-III component

increased sodium tolerance 1 homolog

overexpressed in lung cancer 1

putative MAPK-activating protein PM28

Also known as

OLC1

Summary

This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Expression

Ubiquitous expression in bone marrow (RPKM 49.3), gall bladder (RPKM 37.7) and 25 other tissues See more

RAKENNE:  https://www.ncbi.nlm.nih.gov/protein/NP_001257904.1

https://www.researchgate.net/figure/Model-of-ESCRT-complex-assembly-The-diagram-is-based-loosely-upon-one-previously_fig1_4978493

Aika erikoinen peptidirakenne!

Rakenne:

• GenPept
  

IST1 homolog isoform a [Homo sapiens]

NCBI Reference Sequence: NP_001257904.1
Identical Proteins FASTA Graphics

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LOCUS       NP_001257904             366 aa            linear   PRI 30-APR-2018
DEFINITION  IST1 homolog isoform a [Homo sapiens].
ACCESSION   NP_001257904
VERSION     NP_001257904.1
DBSOURCE    REFSEQ: accession NM_001270975.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 366)
  AUTHORS   Zhang K, Zhao G, Xiao T, Li P, Song J, Gao Y and Sun K.
  TITLE     [Overexpression of OLC1 in Lung Squamous Cell Carcinoma Tissues is
            Associated with Poor Prognosis of Patients]
  JOURNAL   Zhongguo Fei Ai Za Zhi 20 (5), 346-351 (2017)
   PUBMED   28532543
  REMARK    GeneRIF: The expression of OLC1 in the tumor tissues of lung
            adenocarcinoma is higher than that in squamous cell carcinoma.
REFERENCE   2  (residues 1 to 366)
  AUTHORS   Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ,
            Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS and Frost A.
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in
            fission yeast and human cells
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 114 (11), E2166-E2175 (2017)
   PUBMED   28242692
REFERENCE   3  (residues 1 to 366)
  AUTHORS   Wang J, Shen H, Fu G, Zhao D and Wang W.
  TITLE     Nuclear overexpression of the overexpressed in lung cancer 1
            predicts worse prognosis in gastric adenocarcinoma
  JOURNAL   Oncotarget 8 (6), 9442-9450 (2017)
   PUBMED   28038462
  REMARK    GeneRIF: Nuclear overexpression of OLC1 was associated with
            clinicopathological parameters and could predict a poor overall
            survival in gastric adenocarcinoma.
REFERENCE   4  (residues 1 to 366)
  AUTHORS   Vietri M, Schink KO, Campsteijn C, Wegner CS, Schultz SW, Christ L,
            Thoresen SB, Brech A, Raiborg C and Stenmark H.
  TITLE     Spastin and ESCRT-III coordinate mitotic spindle disassembly and
            nuclear envelope sealing
  JOURNAL   Nature 522 (7555), 231-235 (2015)
   PUBMED   26040712
REFERENCE   5  (residues 1 to 366)
  AUTHORS   Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE and
            Yates JR.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J. Proteome Res. 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   6  (residues 1 to 366)
  AUTHORS   Agromayor M, Carlton JG, Phelan JP, Matthews DR, Carlin LM,
            Ameer-Beg S, Bowers K and Martin-Serrano J.
  TITLE     Essential role of hIST1 in cytokinesis
  JOURNAL   Mol. Biol. Cell 20 (5), 1374-1387 (2009)
   PUBMED   19129480
  REMARK    GeneRIF: hIST1 is essential for cytokinesis in mammalian cells.
REFERENCE   7  (residues 1 to 366)
  AUTHORS   Bajorek M, Morita E, Skalicky JJ, Morham SG, Babst M and Sundquist
            WI.
  TITLE     Biochemical analyses of human IST1 and its function in cytokinesis
  JOURNAL   Mol. Biol. Cell 20 (5), 1360-1373 (2009)
   PUBMED   19129479
  REMARK    GeneRIF: IST1 and CHMP1 act together to recruit and modulate
            specific VPS4 activities required during the final stages of cell
            division.
REFERENCE   8  (residues 1 to 366)
  AUTHORS   Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta
            R, Wang NS and Knepper MA.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J. Am. Soc. Nephrol. 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (residues 1 to 366)
  AUTHORS   Yuan J, Ma J, Zheng H, Shi T, Sun W, Zhang Q, Lin D, Zhang K, He J,
            Mao Y, Gao X, Gao P, Han N, Fu G, Xiao T, Gao Y, Ma D and Cheng S.
  TITLE     Overexpression of OLC1, cigarette smoke, and human lung
            tumorigenesis
  JOURNAL   J. Natl. Cancer Inst. 100 (22), 1592-1605 (2008)
   PUBMED   19001599
  REMARK    GeneRIF: OLC1 (KIAA0174) is a candidate oncogene in lung cancer
            whose expression may be regulated by exposure to cigarette smoke.
REFERENCE   10 (residues 1 to 366)
  AUTHORS   Kaye,F.J.
  TITLE     Defining a candidate lung cancer gene
  JOURNAL   J. Natl. Cancer Inst. 100 (22), 1564-1565 (2008)
   PUBMED   19001597
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA698420.1, BC103745.1 and
            AC009127.10.
            
            Summary: This gene encodes a protein with MIT-interacting motifs
            that interacts with components of endosomal sorting complexes
            required for transport (ESCRT). ESCRT functions in vesicle budding,
            such as that which occurs during membrane abscission in
            cytokinesis. There is a pseudogene for this gene on chromosome 19.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Aug
            2012].
            
            Transcript Variant: This variant (1) encodes isoform a (also known
            as hIST1c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.244497.1,
                                           SRR1803615.123498.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     Protein         1..366
                     /product="IST1 homolog isoform a"
                     /note="IST1 homolog; putative MAPK-activating protein
                     PM28; overexpressed in lung cancer 1; increased sodium
                     tolerance 1 homolog; IST1, endosomal sorting complex
                     required for transport-III component"
                     /calculated_mol_wt=39848
     Region          1..168
                     /region_name="Interaction with CHMP1A and CHMP1B"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P53990.1)"
     Site            4
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000244|PubMed:23186163};
                     propagated from UniProtKB/Swiss-Prot (P53990.1)"
     Region          13..176
                     /region_name="Ist1"
                     /note="Regulator of Vps4 activity in the MVB pathway;
                     pfam03398"
                     /db_xref="CDD:281401"
     Site            43
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphotyrosine. {ECO:0000244|PubMed:15592455};
                     propagated from UniProtKB/Swiss-Prot (P53990.1)"
     Region          323..334
                     /region_name="Type-2 MIT-interacting motif"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P53990.1)"
     Region          350..366
                     /region_name="Interaction with VPS4A, VTA1, MITD1 STAMBP
                     and USP8. {ECO:0000269|PubMed:19129480}"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P53990.1)"
     Region          353..363
                     /region_name="MIT-interacting motif"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P53990.1)"
     CDS             1..366
                     /gene="IST1"
                     /gene_synonym="OLC1"
                     /coded_by="NM_001270975.1:113..1213"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS59272.1"
                     /db_xref="GeneID:9798"
                     /db_xref="HGNC:HGNC:28977"
                     /db_xref="MIM:616434"ORIGIN      
    1 mlgsgfkaer lrvnlrlvin rlkllekkkt elaqkarkei adylaagkde rarirvehii
   61 redylveame ilelycdlll arfgliqsmk eldsglaesv stliwaaprl qsevaelkiv
  121 adqlcakysk eygklcrtnq igtvndrlmh klsveappki lverylieia knynvpyepd
  181 svvmaeappg vetdlidvgf tddvkkggpg rggsggftap vggpdgtvpm pmpmpmpmps
  241 antpfsyplp kgpsdfnglp mgtyqafpni hppqipatpp syesvddina dknissaqiv
  301 gpgpkpeasa klpsrpadny dnfvlpelps vpdtlptasa gastsasedi dfddlsrrfe
  361 elkkkt
//