ESCRT-II, EAP45,(Kr.13q14.3), VPS36, C13orf9, CGI-148 , GLUE-domeeni

ESCRT-II

(EAP45 geenin virallinen nimi on VPS36) 

Also known as EAP45; C13orf9; CGI-145

Summary This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013]

Expression Ubiquitous expression in skin (RPKM 25.0), thyroid (RPKM 15.1) and 25 other tissues See more

Preferred Names

vacuolar protein-sorting-associated protein 36

Names

ELL-associated protein of 45 kDa

ESCRT-II complex subunit VPS36

Conserved Domains (2) summary

cd13226
Location:1 → 119

PH-GRAM-like_Eap45; Pleckstrin homology-like domain or GLUE (GRAM-like ubiquitin-binding in Eap45) domain of Eap45

pfam04157
Location:146 → 360

EAP30; EAP30/Vps36 family

Peptide sequence

https://www.ncbi.nlm.nih.gov/protein/NP_001269098.1

Related articles in PubMed

1. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Alam SL, et al. Nat Struct Mol Biol, 2006 Nov. PMID 17057716 Abstract The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting
2. RAB27A, RAB27B and VPS36 are downregulated in advanced prostate cancer and show functional relevance in prostate cancer cells. Worst TS, et al. Int J Oncol, 2017 Mar. PMID 28197629
3. Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex. Im YJ, et al. Dev Cell, 2008 Jun. PMID 18539118, Free PMC Article
4. Structural insight into the ESCRT-I/-II link and its role in MVB trafficking. Gill DJ, et al. EMBO J, 2007 Jan 24. PMID 17215868, Free PMC Article
5. Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production. Meng B, et al. Retrovirology, 2015 Aug 14. PMID 26268989, Free PMC Article
   

See all (37) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. RAB27A, RAB27B and VPS36 are frequently underexpressed in advanced prostate cancer and are inversely correlated with prostate cancer outcome. There seems to be a close relationship in the expression of RAB27A, RAB27B and VPS36, with RAB27A and RAB27B being dependent on VPS36.
2. mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body
3. Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich.
   

Eap45 in Mammalian ESCRT-II Binds Ubiquitin via a Phosphoinositide-interacting GLUE Domain^*,♦

• Thomas Slagsvold‡, Rein Aasland§, ^‡Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway, the ^§Department of Molecular Biology, University of Bergen, HIB, N-5020 Bergen, Norway, and the ^¶Structural Biology Research Center, Photon Factory, IMSS, KEK, Tsukuba, Ibaraki 305-0801, Japan Abstract Ubiquitination serves as a key sorting signal in the lysosomal degradation of endocytosed receptors through the ability of ubiquitinated membrane proteins to be recognized and sorted by ubiquitin-binding proteins along the endocytic route. The ESCRT-II complex in yeast contains one such protein, Vps36, which harbors a ubiquitin-binding NZF domain* and is required for vacuolar sorting of ubiquitinated membrane proteins.

https://www.researchgate.net/profile/Mark_Van_der_Giezen/publication/49784933/figure/fig1/AS:601633838878722@1520452093285/Model-of-ESCRT-complex-assembly-The-diagram-is-based-loosely-upon-one-previously.png    *

Surprisingly, the presumptive mammalian ortholog Eap45 lacks the ubiquitin-binding module of Vps36, and it is thus not clear whether mammalian ESCRT-II functions to bind ubiquitinated cargo. In this paper, we provide evidence that Eap45 contains a novel ubiquitin-binding domain, GLUE (GRAM-like ubiquitin-binding in Eap45), which binds ubiquitin with similar affinity and specificity as other ubiquitin-binding domains.**

The GLUE domain shares similarities in its primary and predicted secondary structures to phosphoinositide-binding GRAM and PH domains. 

** https://www.cell.com/cms/attachment/2119039383/2088388025/gr2.jpg 

Accordingly, we find that Eap45 binds to a subset of 3-phosphoinositides, suggesting that ubiquitin recognition could be coordinated with phosphoinositide binding.

Furthermore, we show that Eap45 colocalizes with ubiquitinated proteins on late endosomes. These results are consistent with a role for Eap45 in endosomal sorting of ubiquitinated cargo. Received February 9, 2005. Revision received March 7, 2005. The American Society for Biochemistry and Molecular Biology, Inc.

Muistiin 17.5. 2018

ESCRT-II, EAP20, (Kr.17q21.2), VPS25, DERP9, FAP20

ESCRT-II

( EAP20  geenin virallinen nimi on nyt VPS25)

Vacuolar protein sorting 25, human
https://www.ncbi.nlm.nih.gov/gene/84313

Also known as DERP9; EAP20; FAP20

Summary This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

Expression Ubiquitous expression in esophagus (RPKM 14.2), colon (RPKM 12.6) and 25 other tissues See more

Orthologs mouse all

Preferred Names

vacuolar protein-sorting-associated protein 25

Names

ELL-associated protein of 20 kDa

ESCRT-II complex subunit VPS25

dermal papilla-derived protein 9

https://www.cell.com/cms/attachment/2119039383/2088388025/gr2.jpg

Conserved Domains (1) summary

pfam05871
Location:10 → 145 ESCRT-II; ESCRT-II complex subunit

Peptide sequence

vacuolar protein-sorting-associated protein 25 [Homo sapiens]

NCBI Reference Sequence: NP_115729.1

https://www.ncbi.nlm.nih.gov/protein/NP_115729.1

Related articles in PubMed

1. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Im YJ, et al. Dev Cell, 2009 Aug. PMID 19686684, Free PMC Article
2. Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes. Sharma M, et al. J Cell Biol, 2004 Mar 15. PMID 15007060, Free PMC Article
3. Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting. Yorikawa C, et al. Biochem J, 2005 Apr 1. PMID 15511219, Free PMC Article
4. Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex. Im YJ, et al. Dev Cell, 2008 Jun. PMID 18539118, Free PMC Article
5. Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production. Meng B, et al. Retrovirology, 2015 Aug 14. PMID 26268989, Free PMC Article
   

See all (35) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
2. Observational study of gene-disease association. (HuGE Navigator)
3. Results describe the mapping of multivesicular bodies with the second winged helix domain of human ESCRT-II subunit VPS25 and the first helix of ESCRT-III subunit VPS20.
4. mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body
   

Muistiin 17.5. 2018

ESCRT-II , EAP30 (Kr.17q21.32), VPS22, SNF8, Dot3

ESCRT-II Complex EAP30

 Geenin viralliseksi nimeksi on päätetty SNF8.

https://www.ncbi.nlm.nih.gov/gene/11267

Also known as

Dot3; EAP30; VPS22

Summary The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] Expression

Ubiquitous expression in kidney (RPKM 18.5), fat (RPKM 18.4) and 25 other tissues See more Orthologs mouse all

Preferred Names

vacuolar-sorting protein SNF8

Names

EAP30 subunit of ELL complex

ELL-associated protein of 30 kDa

ESCRT-II complex subunit VPS22

SNF8, ESCRT-II complex subunit, homolog

EAP30/Vps36 family

This family includes EAP30 as well as the Vps36 protein. Vps36 is involved in Golgi to endosome trafficking. EAP30 is a subunit of the ELL complex. The ELL is an 80-kDa RNA polymerase II transcription factor. ELL interacts with three other proteins to form the complex known as ELL complex. The ELL complex is capable of increasing that catalytic rate of transcription elongation, but is unable to repress initiation of transcription by RNA polymerase II as is the case of ELL. EAP30 is thought to lead to the derepression of ELL's transcriptional inhibitory activity.

Peptide sequence

https://www.ncbi.nlm.nih.gov/protein/NP_001304121.1

Related articles in PubMed

1. Differential functions of Hrs and ESCRT proteins in endocytic membrane trafficking. Raiborg C, et al. Exp Cell Res, 2008 Feb 15. PMID 18031739
2. Pivotal role for the ESCRT-II complex subunit EAP30/SNF8 in IRF3-dependent innate antiviral defense. Kumthip K, et al. PLoS Pathog, 2017 Oct. PMID 29084253, Free PMC Article
3. Inhibition of ESCRT-II-CHMP6 interactions impedes cytokinetic abscission and leads to cell death. Goliand I, et al. Mol Biol Cell, 2014 Nov 15. PMID 25232011, Free PMC Article
4. Identification of CAD candidate genes in GWAS loci and their expression in vascular cells. Erbilgin A, et al. J Lipid Res, 2013 Jul. PMID 23667179, Free PMC Article
5. RILP interacts with the VPS22 component of the ESCRT-II complex. Progida C, et al. Biochem Biophys Res Commun, 2006 Sep 8. PMID 16857164
   

See all (46) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. data describe an unappreciated role for EAP30 in IRF3-dependent innate antiviral response in the nucleus.
2. Taken together, these data suggest an active role for ESCRT-II and CHMP6 in ESCRT-mediated abscission
3. Modulating SNF8 expression levels alters the TRPC6 channel current and can modulate activation of NFAT-mediated transcription downstream of gain-of-function mutant TRPC6.
4. Vps22 has an important role in ligand-induced EGFR and CXCR4 turnover; termination of EGF signaling occurs prior to ESCRT-II engagement
5. mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body
6. there are probably multiple pathways for protein sorting/multivesicular body vesicle formation in human cells and that human immunodeficiency virus type 1 does not utilize an ESCRT-II-dependent pathway to leave the cell