ESCRT-0, VHS domeenit kooperatiossa sitovat ubikitinoituja lasteja

http://emboj.embopress.org/content/29/6/1045.figures-only

VHS domains of ESCRT‐0 cooperate in high‐avidity binding to polyubiquitinated cargo

Xuefeng Ren, James H Hurley

DOI 10.1038/emboj.2010.6 | Published online 11.02.2010

The EMBO Journal (2010) 29, 1045-1054

ESCRT-0, STAM1, (Kr.10p12.33), HSE1, (SH3 domeeni ja ITAM motiivi)

ESCRT-0, STAM1 (Kr.10p12.33), HSE1 , SH3 domeeni ja ITAM motiivi

https://www.ncbi.nlm.nih.gov/gene/8027

Also known as STAM1; STAM-1

Summary This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate (HRS) to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]

Expression Ubiquitous expression in bone marrow (RPKM 13.8), brain (RPKM 7.5) and 25 other tissues See more Orthologs mouse all

Preferred Names

signal transducing adapter molecule 1

Names

HSE1 homolog

signal transducing adaptor molecule (SH3 domain and ITAM motif) 1

Peptide sequence

https://www.ncbi.nlm.nih.gov/protein/NP_001311212.1

Related articles in PubMed

1. Backbone 1H, 13C, and 15N assignments for the tandem ubiquitin binding domains of signal transducing adapter molecule 1(STAM1). Lim J, et al. Biomol NMR Assign, 2011 Apr. PMID 20927613
2. Conformational and dynamic changes at the interface contribute to ligand binding by ubiquitin. Sundd M. Biochemistry, 2012 Oct 16. PMID 23035694
3. Solution structure of UIM and interaction of tandem ubiquitin binding domains in STAM1 with ubiquitin. Lim J, et al. Biochem Biophys Res Commun, 2011 Feb 4. PMID 21187078
4. Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy. Hong YH, et al. FEBS Lett, 2009 Jan 22. PMID 19111546
5. AMSH is an endosome-associated ubiquitin isopeptidase. McCullough J, et al. J Cell Biol, 2004 Aug 16. PMID 15314065, Free PMC Article
   

See all (78) citations in PubMed See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. The beta-arrestin1.STAM1 complex is necessary for promoting autophosphorylation of focal adhesion kinase (FAK). FAK is necessary for CXCL12-induced chemotaxis and associates with and localizes with beta-arrestin1 and STAM1 in a CXCL12-dependent manner.
2. The VHS domain of STAM1 directs AMSH to cleave longer Lys63-linked ubiquitin chains
3. Differential expression of the transcripts STAM connects ubiquitin-proteasome system with infection-inflammation in preterm births and preterm premature rupture of membranes.
4. ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation.
5. Overexpression of an AIP4 catalytically inactive mutant and a mutant that shows poor binding to STAM-1 fails to enhance CXCR4-induced ERK-1/2 signaling.
6. Observational study of gene-disease association. (HuGE Navigator)
7. A novel ubiquitin binding site and the manner of ubiquitin recognition of the STAM1 VHS domain were proposed.
8. STAMs function prominently in endoplasmic reticulum-to-Golgi trafficking, most likely through direct interactions with the coat protein II complex
9. The cellular functions of UBPY are complex but clearly distinct from those of the Lys-63-ubiquitin-specific protease, AMSH, with which it shares a binding site on the SH3 domain of STAM
10. Analysis with phospho-specific antibodies indicates that 3 kinases generate a signal-specific, combinatorial phosphorylation profile of the Hrs-STAM complex, with the potential of diversifying tyrosine kinase receptor signalling through a common element.
    

Submit: New GeneRIF Correction See all GeneRIFs (14)

ESCRT-0 vaiheesta kuva

https://openi.nlm.nih.gov/detailedresult.php?img=PMC2172707_200302136f7&req=4

 ( Vps27 rekrytoi seuraavan kompleksi ESCRT-1).

 


fig7: Model for compartment specification of Ub-dependent sorting into MVB vesicles. The Vps15/Vps34 complex synthesizes PI(3)P on endosomal membranes. The class E Vps protein Vps27 is targeted to PI(3)P- containing endosomal membranes via its FYVE domain (dark blue) where it can bind ubiquitinated MVB cargo via its UIM motifs (yellow). 
Vps27 subsequently recruits/activates ESCRT-I (orange) on endosomes. Ubiquitinated cargo (such as pCPS) is recognized by ESCRT-I (via the Ub E2 variant domain of Vps23), which initiates cargo entry into MVB vesicles. The action of a number of additional class E Vps proteins (ESCRT-II and ESCRT-III) is required for not only the function of this pathway but also for recruiting the deubiquitinating enzyme to remove Ub from cargo before its entry into invaginating vesicles. The concerted action of these proteins results in the sorting of cargo into the MVB pathway.  


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1648078/ 
Taulukon alkuosa ESCRT-0  ei ole  kovin helppoa hamottaa, sillä siihen  konvertoituu monenlaista tekijää, Olen nyt katosnut HRS geenin. Nyt katson  STAM geenit. 

Table 1

Class E Vps proteins and Complexes

Complex	Yeast protein	Human protein	Motifs	Binds to
Vps27-Hse1 complex	Vps27	HRS	UIM, FYVE, VHS	Ubiquitin, PI(3)P, ESCRT-II (Vps23)
	Hse1	STAM1, STAM2	UIM, VHS, SH3	Ubiquitin
ESCRT-I complex	Vps23	TSG101	UEV	Ubiquitin, Vps27
	Vps28	VPS28
	Vps37	VPS37A, B, C, D	Coiled-coil
ESCRT-II complex	Vps22	EAP30	Coiled-coil
	Vps25	EAP25		ESCRT-III (Vps20)
	Vps36	EAP45	GLUE, NZF	Ubiquitin
ESCRT-III complex	Vps2/Did4	CHMP2A, B	Charged, Coiled-coil
	Vps20	CHMP6	Charged, Coiled-coil	ESCRT-II (Vps25)
	Vps24	CHMP3	Charged, Coiled-coil
	Snf7/Vps32	CHMP4A, B, C	Charged, Coiled-coil
Vps4 complex	Vps4	VPS4A, B	AAA ATPase, Coiled-coil	ESCRT-III
Other MVB Proteins	Bro1/Vps31	ALIX/AIP1	Charged, Coiled-coil	LBPA, Doa4, ESCRT-III
	Vps60/Mos10	CHMP5	Charged, Coiled-coil	ESCRT-III
	Fti1/Did2	CHMP1A, B	Charged, Coiled-coil	ESCRT-III
	Vta1	LIP5		Vps4
	Vps44/Nhx1	SLC9A6	Sodium/Proton Exchanger

Open in a separate window