KLHL13(Xq24), BKLHD2. Tarkka kromosomisegrekaatio ja sytokineesi

https://www.ncbi.nlm.nih.gov/gene/90293

Also known as

BKLHD2

Summary

This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Expression Broad expression in endometrium (RPKM 9.9), kidney (RPKM 3.7) and 15 other tissues See more

Preferred Names

kelch-like protein 13

Names

BTB and kelch domain containing 2

kelch-like 13

1. A Cul3-based E3 ligase regulates mitosis and is required to maintain the spindle assembly checkpoint in human cells. Sumara I, et al. Cell Cycle, 2007 Dec 15. PMID 18075312. The spindle assembly checkpoint (SAC) is a mechanism that prevents premature chromosome segregation in anaphase before all chromosomes are correctly attached to the mitotic spindle. Errors in chromosome segregation lead to aneuploidy, which may be causally involved in tumorgenesis. Kinetochore complexes are the structural components of the SAC, which are tightly regulated by various mechanisms including phosphorylation and ubiquitin-dependent proteolysis. Recent studies shed new light on the regulatory pathways of the ubiquitin proteasome system involved in SAC signaling. Here we present evidence that a Cul3-based E3 ubiquitin-ligase is required to maintain SAC signaling in human cells. Inactivation of the Cul3/KLHL9/KLHL13 ligase leads to premature degradation of Cyclin B and exit from the mitotic state in the presence of microtubule poisons. We discuss possible mechanisms how Cul3 may be required to maintain SAC activity by ubiquitination of the chromosomal passenger protein Aurora B. 
2.  
3. A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells. Sumara I, et al. Dev Cell, 2007 Jun. PMID 17543862. Faithful cell-cycle progression is tightly controlled by the ubiquitin-proteasome system. Here we identify a human Cullin 3-based E3 ligase (Cul3) which is essential for mitotic division. In a complex with the substrate-specific adaptors KLHL9 and KLHL13, Cul3 is required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis. This Cul3-based E3 ligase removes components of the chromosomal passenger complex from mitotic chromosomes and allows their accumulation on the central spindle during anaphase. Aurora B directly binds to the substrate-recognition domain of KLHL9 and KLHL13 in vitro, and coimmunoprecipitates with the Cul3 complex during mitosis. Moreover, Aurora B is ubiquitylated in a Cul3-dependent manner in vivo, and by reconstituted Cul3/KLHL9/KLHL13 ligase in vitro. We thus propose that the Cul3/KLHL9/KLHL13 E3 ligase controls the dynamic behavior of Aurora B on mitotic chromosomes, and thereby coordinates faithful mitotic progression and completion of cytokinesis. 
4.  
5. USP25 regulates Wnt signaling by controlling the stability of tankyrases. Xu D, et al. Genes Dev, 2017 May 15. PMID 28619731, Free PMC Article
6.  
7. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis. Maerki S, et al. J Cell Biol, 2009 Dec 14. PMID 19995937, Free PMC ArticleCul3 (Cullin3)-based E3 ubiquitin ligases recently emerged as critical regulators of mitosis. In this study, we identify two mammalian BTB (Bric-a-brac-Tramtrack-Broad complex)-Kelch proteins, KLHL21 and KLHL22, that interact with Cul3 and are required for efficient chromosome alignment. Interestingly, KLHL21 but not KLHL22 is necessary for cytokinesis and regulates translocation of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase, similar to the previously described BTB-Kelch proteins KLHL9 and KLHL13. KLHL21 directly binds to aurora B and mediates ubiquitination of aurora B in vitro. In contrast to KLHL9 and KLHL13, KLHL21 localizes to midzone microtubules in anaphase and recruits aurora B and Cul3 to this region. Together, our results suggest that different Cul3 adaptors nonredundantly regulate aurora B during mitosis, possibly by ubiquitinating different pools of aurora B at distinct subcellular localizations.
8.  
9. Dimerization quality control ensures neuronal development and survival. Mena EL, et al. Science, 2018 Oct 12. PMID 30190310

See all (28) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

KLHL9 (9p2.3) . Solusyklin säätelijäkompleksin adaptoriosa mitoosissa ja sytokineesissä

https://www.ncbi.nlm.nih.gov/gene/55958

Summary This gene encodes a protein that belongs to the kelch repeat-containing family, and contains an N-terminal BTB/POZ domain, a BACK domain and six C-terminal kelch repeats. The encoded protein is a component of a complex with cullin 3-based E3 ligase, which plays a role in mitosis. This protein complex is a cell cycle regulator, and functions in the organization and integrity of the spindle midzone in anaphase and the completion of cytokinesis. The complex is required for the removal of the chromosomal passenger protein aurora B from mitotic chromosomes. [provided by RefSeq, Jul 2016]

Orthologs mouse all

 

ORIGIN      
        1 mkvslgngem gvsahlqpck agttrfftsn thssvvlqgf dqlriegllc dvtlvpgdgd
       61 eifpvhramm asasdyfkam ftggmkeqdl mciklhgvnk vglkkiidfi ytaklslnmd
      121 nlqdtleaas flqilpvldf ckvflisgvs ldncvevgri antynlievd kyvnnfilkn
      181 fpallstgef lklpferlaf vlssnslkhc telelfkaac rwlrledprm dyaaklmkni
      241 rfplmtpqdl inyvqtvdfm rtdntcvnll leasnyqmmp ymqpvmqsdr tairsdsthl
      301 vtlggvlrqq lvvskelrmy deraqewrsl apmdapryqh giavignfly vvggqsnydt
      361 kgktavdtvf rfdprynkwm qvaslnekrt ffhlsalkgh lyavggrsaa gelatvecyn
      421 prmnewsyva kmsephygha gtvygglmyi sggithdtfq nelmcfdpdt dkwmqkapmt
      481 tvrglhcmct vgdklyvigg nhfrgtsdyd dvlsceyysp tldqwtpiaa mlrgqsdvgv
      541 avfenkiyvv ggyswnnrcm veivqkydpe kdewhkvfdl peslggirac tltvfppeen
      601 pgspsrespl sapsdhs
//

1. Kelch-like homologue 9 mutation is associated with an early onset autosomal dominant distal myopathy. Cirak S, et al. Brain, 2010 Jul. PMID 20554658, Free PMC Article
2. Pan-genome isolation of low abundance transcripts using SAGE tag. Kim YC, et al. FEBS Lett, 2006 Dec 11. PMID 17113583, Free PMC Article
3. Ubiquitylation-dependent localization of PLK1 in mitosis. Beck J, et al. Nat Cell Biol, 2013 Apr. PMID 23455478
4. A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells. Sumara I, et al. Dev Cell, 2007 Jun. PMID 17543862. Faithful cell-cycle progression is tightly controlled by the ubiquitin-proteasome system. Here we identify a human Cullin 3-based E3 ligase (Cul3) which is essential for mitotic division. In a complex with the substrate-specific adaptors KLHL9 and KLHL13, Cul3 is required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis. This Cul3-based E3 ligase removes components of the chromosomal passenger complex from mitotic chromosomes and allows their accumulation on the central spindle during anaphase. Aurora B directly binds to the substrate-recognition domain of KLHL9 and KLHL13 in vitro, and coimmunoprecipitates with the Cul3 complex during mitosis. Moreover, Aurora B is ubiquitylated in a Cul3-dependent manner in vivo, and by reconstituted Cul3/KLHL9/KLHL13 ligase in vitro. We thus propose that the Cul3/KLHL9/KLHL13 E3 ligase controls the dynamic behavior of Aurora B on mitotic chromosomes, and thereby coordinates faithful mitotic progression and completion of cytokinesis.
5. USP25 regulates Wnt signaling by controlling the stability of tankyrases. Xu D, et al. Genes Dev, 2017 May 15. PMID 28619731, Free PMC Article

See all (27) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene