TRIM70 (Kr.17p11.2) , TRIM16L

TRIM70 alias TRIM16L (TRIM16-like tripartite motif protein)

(Kr. 17p11.2)

Tämän TRIM70 rakenne kuuluu C- UC, luokittelemattomiin ja katsotaan TRIM16-kaltaiseksi. Siinä ei ole RING domeenia , mutta löytyy LXXLL motiivi ja runsas PRYSPRY. Geeniä ilmenee monessa kudoksessa ( 24 kudosta) ja eniten ruokatorvessa. Solunsisällä se sijoittuu sytoplasmaan ja plasma kalvoon. Otan sitaatin 1-isoformista tähän mukaan. Vähän ihmettelen assosiaatiota tässä yhteydessä TRIM59 proteiiniin, joka on kromosomista 3 (TSBF1, EXI TN, RNF194) ja sen takia siteeraan artikkelin TRIMsuperperheen seulonnasta NSCLC.ssä. TRIM70 on niitä trimejä, jotka säätyvät merkitsevästi ylös ei-pienisoluisessa keuhkokarsionoomassa TRIM59:n ohella.

https://www.ncbi.nlm.nih.gov/gene/?term=TRIM70

Expression Ubiquitous expression in esophagus (RPKM 13.7), gall bladder (RPKM 5.6) and 24 other tissues See more

Conserved Domains (1) summary

cd12890
Location:149 → 330

SPRY_PRY_TRIM16; PRY/SPRY domain in tripartite motif-containing protein 16 (TRIM16)

ISOFORMI 1 rakenne: 1- 348 aminohappoa

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LOCUS       NP_001032407             348 aa            linear   PRI 28-DEC-2017
DEFINITION  tripartite motif-containing protein 16-like protein isoform 1 [Homo
            sapiens].
ACCESSION   NP_001032407 XP_085722
VERSION     NP_001032407.1
DBSOURCE    REFSEQ: accession NM_001037330.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 348)
  AUTHORS   Zhan W, Han T, Zhang C, Xie C, Gan M, Deng K, Fu M and Wang JB.
  TITLE     TRIM59 Promotes the Proliferation and Migration of Non-Small Cell
            Lung Cancer Cells by Upregulating Cell Cycle Related Proteins
  JOURNAL   PLoS ONE 10 (11), e0142596 (2015)
   PUBMED   26599082
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK301647.1, DQ232882.1 and BQ669526.1.
            On Nov 24, 2005 this sequence version replaced XP_085722.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK301647.1, DQ232882.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     Protein         1..348
                     /product="tripartite motif-containing protein 16-like
                     protein isoform 1"
                     /note="tripartite motif-containing protein 16-like
                     protein; tripartite motif protein 70; tripartite
                     motif-containing protein 70"
                     /calculated_mol_wt=40175
     Region          149..330
                     /region_name="SPRY_PRY_TRIM16"
                     /note="PRY/SPRY domain in tripartite motif-containing
                     protein 16 (TRIM16); cd12890"
                     /db_xref="CDD:293948"
     CDS             1..348
                     /gene="TRIM16L"
                     /gene_synonym="TRIM70"
                     /coded_by="NM_001037330.2:344..1390"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS32588.1"
                     /db_xref="GeneID:147166"
                     /db_xref="HGNC:HGNC:32670"
ORIGIN      
        1 mqfgellaav rkaqanvmlf leekeqaals qangikahle yrsaemeksk qeletmaais
       61 ntvqfleeyc kfknteditf psvyiglkdk lsgirkvite stvhliqlle nykkklqefs
      121 keeeydirtq vsaivqrkyw tskpepstre qflqyvhdit fdpdtahkyl rlqeenrkvt
      181 nttpwehpyp dlpsrflhwr qvlsqqslyl hryyfeveif gagtyvgltc kgidqkgeer
      241 sscisgnnfs wslqwngkef tawysdmetp lkagpfwrlg vyidfpggil sfygveydsm
      301 tlvhkfackf sepvyaafwl skkenairiv dlgeepekpa pslvgtap
//

Muita lähteitä Googlesta:

https://www.proteinatlas.org/ENSG00000108448-TRIM16L/pathology

Non Solved Structure Found.

https://www.phosphosite.org/images/struct_unavailable.jpg

https://www.phosphosite.org/proteinAction?id=22407&showAllSites=true

Predicted pI for various phosphorylation sites

http://www.uniprot.org/uniprot/Q309B1

• PubMed haku TRIM16L:

FISH TRIM16Lvain 29% samankaltaisuus ihmisen TRIM16L kanssa

https://www.ncbi.nlm.nih.gov/pubmed/?term=TRIM16L

TRIMproteiinien superperheen seulonnassa TRIM70(TRIM16L) ja TRIM16 säätyvät ylös ei-pienisoluisessa keuhkosyövässä.

https://www.ncbi.nlm.nih.gov/pubmed/?term=TRIM70

TRIM70 esiintyy artikkelissa jossa tutkitaan TRIM-superperheen profiilia ei-pienisoluisessa keuhkosyövääsä. Merkitsevästi ylössäätyneet NSCLC-syövässä olivat TRIM-geenit:

11. kromosomista TRIM3 (IG-FLMN), TRIM21(RoSSA), TRIM22 (GPSTAF50). TRIM29 (ATDC) , TRIM66 (TIF1D)

5. kromosomista TRIM7 (GNIP, glykogeniinin kanssa interaktion tekevä proteiini),

9. kromosomista TRIM14 (KIAA0129),

17. kromosomista TRIM16 (EBBP), TRIM70 (TRIM16L)

3. kromosomista TRIM59 (tuumorisuppressori TSBF1, Mrf1,IFT)

Merkitsevästi alassäätyneet NSCLC:ssa olivat TRIM-geenit:

7.kromosomsita TRIM4 (RNF87),

14. kromosomsita TRIM9 (RNF91. SPRING)

5. kromosomista TRIM36 (RNF98,HAPRIN,ANPH) ja TRIM76 (Myospryn, CMYA5, SPRYD2).

1. kromosomista TRIM46 (TRIFIC, GENEY) ja TRIM67 (TNL, TRIM9L)

2. kromosomista TRIM54 (RNF30, MURF3)

• PLoS One. 2015 Nov 24;10(11):e0142596. doi: 10.1371/journal.pone.0142596. eCollection 2015.TRIM59 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer Cells by Upregulating Cell Cycle Related Proteins.Zhan W¹, Han T¹, Zhang C¹, Xie C¹, Gan M¹, Deng K¹, Fu M², Wang JB¹. AbstractTRIM protein family is an evolutionarily conserved gene family implicated in a number of critical processes including inflammation, immunity, antiviral and cancer. In an effort to profile the expression patterns of TRIM superfamily in several non-small cell lung cancer (NSCLC) cell lines, we found that the expression of 10 TRIM genes including TRIM3, TRIM7, TRIM14, TRIM16, TRIM21, TRIM22, TRIM29, TRIM59, TRIM66 and TRIM70 was significantly upregulated in NSCLC cell lines compared with the normal human bronchial epithelial (HBE) cell line, whereas the expression of 7 other TRIM genes including TRIM4, TRIM9, TRIM36, TRIM46, TRIM54, TRIM67 and TRIM76 was significantly down-regulated in NSCLC cell lines compared with that in HBE cells. As TRIM59 has been reported to act as a proto-oncogene that affects both Ras and RB signal pathways in prostate cancer models, we here focused on the role of TRIM59 in the regulation of NSCLC cell proliferation and migration. We reported that TRIM59 protein was significantly increased in various NSCLC cell lines. SiRNA-induced knocking down of TRIM59 significantly inhibited the proliferation and migration of NSCLC cell lines by arresting cell cycle in G2 phase. Moreover, TRIM59 knocking down affected the expression of a number of cell cycle proteins including CDC25C and CDK1. Finally, we knocked down TRIM59 and found that p53 protein expression levels did not upregulate, so we proposed that TRIM59 may promote NSCLC cell growth through other pathways but not the p53 signaling pathway.