TRIM49 (Kr.11q14.3) , RNF18; TRIM49A; TRIM49L2 , C_IV, SPRY)
- Tämä TRIM49 on uusia geenejä, ja se on paikallistettu lähelle sentromeeriä, asemaan 11q14.3. Rakenteellisesti on sanottu sen musituttavan TRIM21:tä, RoSSA:a.
- TRIM49 omaa rakenteen, jossa on 452 aminohappoa ja RING-Zn finger , sinkkijonia sitovia kohtia. Se on motiivi, jonka tiedetään tekevän proteiini-proteiini-interaktioita. Asemassa 285- 445 on SPRY-domeeni. Genin ilmenemä on matala. kahdessa kudoksesas..
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- Summary The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This gene has been found to be preferentially expressed in testis. Related pseudogenes and gene duplicates have also been identified on chromosome 11. [provided by RefSeq, Aug 2010] Expression Low expression observed in reference dataset See more
UNIPROT .Protein
Domains for TRIM49 Gene
http://www.genecards.org/cgi-bin/carddisp.pl?gene=TRIM49
- InterPro:
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- ConA-like_dom_sf
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- Znf_RING/FYVE/PHD
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- Znf_RING
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- B30.2/SPRY
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- SPRY_dom
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- See all 7 »
TRIM49, uusi proteiini RNF18, koodattiin vuonna 2000.
- Isolation of a cDNA for a novel human RING finger protein gene, RNF18, by the virtual transcribed sequence (VTS) approach(1). Yoshikawa T, et al. Biochim Biophys Acta, 2000 Oct 2. PMID 11018261 .
Tutkijat
kehittivät uuden tietuejärjestelmän ja eristivät kehittämällään
järejstelmällä uuden geenin, jolla oli RING finger motiivi
(C(3)HC(4) eikä tätä oltu aiemmin kortistoitu. He antoivat
proteiinille nimen RNF18. Tietueista he päättelivät tämän
geenin omavan samanlaisuukia SS-A/Ro52 proteiinin kanssa (TRIM21,
joka on ribonukleoproteiini autoantigeeni ja neutraloi vasta-aineista
riippuvia patogeeneja; reagoi Sjögrenin oireyhtymää ja SLE-tautia
potevilla autovasta-aineiden kanssa). TRIM49 (RNF18) mRNA esiintyi
lähinnä testiksessä. Kromosomaalista paikallistumista tutkittaessa
havaittiin geenin sijoituvan sentromeerialueelle kromosomissa 11.
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Abstract We have recently developed a novel database system, designated as the virtual transcribed sequence (VTS) which efficiently extracts many genes from public human genome databases, and tested the feasibility of this novel computational approach (N. Miyajima, C. Burge, T. Saito, Biochem. Biophys. Res. Commun. 272 (2000) 801; http://host45.maze.co.jp/vts/). In this study, using the VTS approach, we isolated a cDNA for a novel human gene with RING finger motif (C(3)HC(4)), which is not deposited in public EST databases. The isolated cDNA clone is 2163 bp in length, and contains an open reading frame of 452 amino acids. We designated the novel gene as RNF18. A database search showed that the RNF18 gene had the moderate similarity to SS-A/Ro52 protein (TRIM21) , which is a ribonucleoprotein reactive with autoantibodies in patients with Sjögren's syndrome and systemic lupus erythematosus. Tissue distribution analyses by Northern blot and RT-PCR methods demonstrated that the RNF18 TRIM49) messenger RNA was preferentially expressed in testis. The exon-intron boundaries of RNF18 gene were determined by aligning the cDNA sequence with the corresponding genome sequence. The isolated cDNA consists of eight exons that span about 11 kb of the genome DNA. The precise chromosomal location of the RNF18 gene was determined by PCR-based radiation hybrid mapping, and the gene was located to centromere region of chromosome 11 between markers NIB1900 and D11S1350. Taken together, the VTS approach should provide a novel cDNA cloning strategy for isolating unidentified genes, which are not found even in EST databases but are detectable computationally.
PubMed Gene TRIM49
Prote Johansen T and Deretic V.in TITLE TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition JOURNAL Dev. Cell 30 (4), 394-409 (2014) PUBMED 25127057 REFERENCE 2 (residues 1 to 452) AUTHORS Niu N, Schaid DJ, Abo RP, Kalari K, Fridley BL, Feng Q, Jenkins G, Batzler A, Brisbin AG, Cunningham JM, Li L, Sun Z, Yang P and Wang L. TITLE Genetic association with overall survival of taxane-treated lung cancer patients - a genome-wide association study in human lymphoblastoid cell lines followed by a clinical association study JOURNAL BMC Cancer 12, 422 (2012) PUBMED 23006423 REMARK Publication Status: Online-Only REFERENCE 3 (residues 1 to 452) AUTHORS Han K, Lou DI and Sawyer SL. TITLE Identification of a genomic reservoir for new TRIM genes in primate genomes JOURNAL PLoS Genet. 7 (12), e1002388 (2011) PUBMED 22144910 REFERENCE 4 (residues 1 to 452) AUTHORS Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, Lloyd C, Itoh T, Takeda T, Kim DW, She X, Barlow KF, Bloom T, Bruford E, Chang JL, Cuomo CA, Eichler E, FitzGerald MG, Jaffe DB, LaButti K, Nicol R, Park HS, Seaman C, Sougnez C, Yang X, Zimmer AR, Zody MC, Birren BW, Nusbaum C, Fujiyama A, Hattori M, Rogers J, Lander ES and Sakaki Y. TITLE Human chromosome 11 DNA sequence and analysis including novel gene identification JOURNAL Nature 440 (7083), 497-500 (2006) PUBMED 16554811 REFERENCE 5 (residues 1 to 452) AUTHORS Yoshikawa T, Seki N, Azuma T, Masuho Y, Muramatsu M, Miyajima N and Saito T. TITLE Isolation of a cDNA for a novel human RING finger protein gene, RNF18, by the virtual transcribed sequence (VTS) approach(1) JOURNAL Biochim. Biophys. Acta 1493 (3), 349-355 (2000) PUBMED 11018261 COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB037682.1. On Sep 20, 2006 this sequence version replaced XP_946964.1. Summary: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This gene has been found to be preferentially expressed in testis. Related pseudogenes and gene duplicates have also been identified on chromosome 11. [provided by RefSeq, Aug 2010]. ##Evidence-Data-START## Transcript exon combination :: AB037682.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2145743 [ECO:0000350] ##Evidence-Data-END## FEATURES Location/Qualifiers source 1..452 /organism="Homo sapiens" /db_xref="taxon:9606" /chromosome="11" /map="11q14.3" Protein 1..452 /product="tripartite motif-containing protein 49" /note="ring finger protein 18; testis-specific RING Finger protein; testis-specific ring-finger protein" /calculated_mol_wt=52757 Region 12..57 /region_name="RING-HC_TRIM43_like_C-IV" /note="RING finger, HC subclass, found in tripartite motif-containing proteins TRIM43, TRIM48, TRIM49, TRIM51, TRIM64, TRIM77 and similar proteins; cd16603" /db_xref="CDD:319517" Region 15..55 /region_name="RING-HC finger (C3HC4-type)" /note="RING-HC finger (C3HC4-type) [structural motif]" /db_xref="CDD:319517" Site order(15,18,30,32,35,38,52,55) /site_type="other" /note="Zn binding site [ion binding]" /db_xref="CDD:319517" Region 285..448 /region_name="SPRY" /note="SPRY domain; cl02614" /db_xref="CDD:322017" CDS 1..452 /gene="TRIM49" /gene_synonym="RNF18; TRIM49A; TRIM49L2" /coded_by="NM_020358.2:330..1688" /db_xref="CCDS:CCDS8287.1" /db_xref="GeneID:57093" /db_xref="HGNC:HGNC:13431" /db_xref="MIM:606124" ORIGIN 1 mnsgilqvfq gelicplcmn yfidpvtidc ghsfcrpcfy lnwqdipflv qcsectkste 61 qinlktnihl kkmaslarkv slwlflssee qmcgthretk kifcevdrsl lcllcsssqe 121 hryhrhrpie waaeehrekl lqkmqslwek acenhrnlnv ettrtrcwkd yvnlrleair 181 aeyqkmpafh heeekhnlem lkkkgkeifh rlhlskakma hrmeilrgmy eelnemchkp 241 dvellqafgd ilhrsesvll hmpqplnpel sagpitglrd rlnqfrvhit lhheeanndi 301 flyeilrsmc igcdhqdvpy ftatprsfla wgvqtftsgk yywevhvgds wnwafgvcnm 361 yrkeknqnek idgkaglfll gcvkndiqcs lfttsplmlq yipkptsrvg lfldceaktv 421 sfvdvnqssl iytipncsfs pplrpifcci hf //
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