TRIM31 (Kr.6p22.1), RNF,HCG1, (Alaryhmä CV)

TRIM31 (Kr.6p22.1), RNF, HCGI, HCG1, C6orf13, (C V)

Tämä TRIM31 kuuluu alaryhmään C V ja siinä näyttää olevan  konservatiivisistä domeeneista RING ja B-box2 sekä Coiled. Coil alue (C-C). (Samanlaista CV-ryhmän domaanirakennetta on myös TRIM 40, 56,73 ja 74).
Tälla geenillä on havaittu noussutta ilmenemää eräissä tuumoreissa ja se saattaa olla solukasvun negatiivinen säätelijä. Alternatiivisella pleissauksella tulee useita transkriptivariantteja. Geeniä esiintyy paksusuolessa, virtsarakossa ja hieman myös 5 muussa kudoksessa ( nämä ovat pohjukaissuoli, ohutsuoli, mahalaukku, umpisuoli ja testis). Geenin alternatiivi nimi HCG1 tulee sanoista Hemochromatosis Candidate Gene I. TRIM31 sijoittuu sytoplasmisesti ja joltain fraktiolta mahdollisesti assosioituu mitokondriaan. TRIM31 pitoisuuden säätyminen on monimutkainen. Sitä on havaittu kohonneena kroonisessa gastriitissa ja mahasyövässä. Se voinee olla gastrisen karsinogeneesin alkuvaiheen merkitsijä. Keuhkosyöpäsolututkimuksissa TRIM31 plasmiditransfektio oli tuumorin kasvua vaimentava , solusyklin säätelijäsykliini D1 ja sykliiniE vähenivät. Pääteltiin, että TRIM31 voi olla tuumorisuppressiivinen NSCLC-keuhkosyövässä. Tämä TRIM on antiretroviraali. Rakenteessa on 425 aminohappoa. Rakenteesta on tarkka selvitys alla. Ehkä  tämä TRIM osallistuu DNA-korjausprosessiin, arvelen.

• https://www.ncbi.nlm.nih.gov/gene/11074#gene-expression
  

RNF; HCG1; HCGI; C6orf13 ; Summary This gene encodes a protein that functions as an E3 ubiquitin-protein ligase. This gene shows altered expression in certain tumors and may be a negative regulator of cell growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Expression ; Biased expression in colon (RPKM 26.2), urinary bladder (RPKM 20.1) and 5 other tissues See more

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Related articles in PubMed

1. TRIM31 is downregulated in non-small cell lung cancer and serves as a potential tumor suppressor. Li H, et al. Tumour Biol, 2014 Jun. PMID 24566900 TRIM31 expression was lower in lung cancer cell lines than normal bronchial cell line HBE. Transfection of TRIM31 plasmid was performed in H157 and H1299 cells. TRIM31 overexpression inhibited cell growth rate and colony formation ability in both cell lines. In addition, expression of cell cycle regulator cyclin D1 and cyclin E were decreased after TRIM31 transfection. In conclusion, TRIM31 might serve as a tumor suppressor in non-small cell lung cancer.
2. The cellular level of TRIM31, an RBCC protein overexpressed in gastric cancer, is regulated by multiple mechanisms including the ubiquitin-proteasome system. Sugiura T. Cell Biol Int, 2011 Jul. PMID 21231912
3. Characterization of TRIM31, upregulated in gastric adenocarcinoma, as a novel RBCC protein. Sugiura T, et al. J Cell Biochem, 2008 Nov 1. PMID 18773414 To explore the molecules associated with gastric adenocarcinoma, we used the gene expression profile database of various human tissues and identified TRIM31 upregulated in both patients with chronic gastritis and stomach cancer. TRIM31 is a new member of RBCC proteins composed of RING finger, B-box and coiled-coil domains. We characterized TRIM31 biochemically and found it possess properties in common with other RBCC proteins, such as occurrence of alternative splicing transcripts, in vitro autoubiquitylating activity and a tendency to homo-oligomerize. The primary localization site of TRIM31 is the cytoplasm but some fraction is potentially associated with the mitochondria. TRIM31 overexpression suppresses colony formation of HCT116 cells while knockdown of its expression with short interfering RNAs (siRNAs) consistently tends to enhance growth of AsPC-1 cells slightly. Thus, TRIM31 is a characteristic RBCC protein with the ability to regulate cell proliferation negatively and may be a potential biomarker of gastric cancer as it is overexpressed from the early stage of gastric carcinogenesis.

4. Localization of seven new genes around the HLA-A locus. el Kahloun A, et al. Hum Mol Genet, 1993 Jan. PMID 8490624 A yeast artificial chromosome (YAC B30) with a 320 kb insert of genomic DNA which includes the HLA-A gene was used to screen a cDNA library of human duodenal mucosa. Seven cDNA clones were isolated which correspond to seven new non-HLA class I structural genes. These new genes are located within a region that may well contain the gene responsible for hemochromatosisand have therefore been named HCG I-VII (Hemochromatosis Candidate Gene).
   

See all (32) citations in PubMed

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. TRIM31 gene expression is regulated by miR-551b in ovarian cancer. ...miR-551b functioned through the suppression of Foxo3 and TRIM31, two important tumor suppressors.
2. TRIM31 directly binds to NSE4, suggesting the existence of a TRIM31-MAGEA1-NSE4 complex reminiscent of the NSE1-NSE3-NSE4 trimer.
   Nse1-Nse3-Nse4.   a tight subcomplex of the structural maintenance of chromosomes SMC5-6 complex.  human SMC5-6 protein complex: Structural maintenance of chromosome.
   (ortholog of Nse3 = MAGEG1 )
   Cohesin:  (Smc1, Smc3), molecular glue, that holds the sister chromatides together for the time that they are established through DNA replication untie they separate in anaphase.
   Condensin:( Smc2 and Smc4), compaction of chromosomes at the beginning of mitosis.
   DNA repair and resolving recombination structures: Smc5-6.
3. Observational study of gene-disease association. (HuGE Navigator)
   

E3 ubiquitin-protein ligase TRIM31 [Homo sapiens]

NCBI Reference Sequence: NP_008959.3
Identical Proteins FASTA Graphics

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LOCUS       NP_008959                425 aa            linear   PRI 11-FEB-2018
DEFINITION  E3 ubiquitin-protein ligase TRIM31 [Homo sapiens].
ACCESSION   NP_008959
VERSION     NP_008959.3
DBSOURCE    REFSEQ: accession NM_007028.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 425)
  AUTHORS   Wei Z, Liu Y, Wang Y, Zhang Y, Luo Q, Man X, Wei F and Yu X.
  TITLE     Downregulation of Foxo3 and TRIM31 by miR-551b in side population
            promotes cell proliferation, invasion, and drug resistance of
            ovarian cancer
  JOURNAL   Med. Oncol. 33 (11), 126 (2016)
   PUBMED   27743201
  REMARK    GeneRIF: TRIM31 gene expression is regulated by miR-551b in ovarian
            cancer.
REFERENCE   2  (residues 1 to 425)
  AUTHORS   Kozakova L, Vondrova L, Stejskal K, Charalabous P, Kolesar P,
            Lehmann AR, Uldrijan S, Sanderson CM, Zdrahal Z and Palecek JJ.
  TITLE     The melanoma-associated antigen 1 (MAGEA1) protein stimulates the
            E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4
            complex
  JOURNAL   Cell Cycle 14 (6), 920-930 (2015)
   PUBMED   25590999
  REMARK    GeneRIF: TRIM31 directly binds to NSE4, suggesting the existence of
            a TRIM31-MAGEA1-NSE4 complex reminiscent of the NSE1-NSE3-NSE4
            trimer.
REFERENCE   3  (residues 1 to 425)
  AUTHORS   Li H, Zhang Y, Zhang Y, Bai X, Peng Y and He P.
  TITLE     TRIM31 is downregulated in non-small cell lung cancer and serves as
            a potential tumor suppressor
  JOURNAL   Tumour Biol. 35 (6), 5747-5752 (2014)
   PUBMED   24566900
  REMARK    GeneRIF: TRIM31 might serve as a tumor suppressor in non-small cell
            lung cancer.
REFERENCE   4  (residues 1 to 425)
  AUTHORS   Uchil PD, Hinz A, Siegel S, Coenen-Stass A, Pertel T, Luban J and
            Mothes W.
  TITLE     TRIM protein-mediated regulation of inflammatory and innate immune
            signaling and its association with antiretroviral activity
  JOURNAL   J. Virol. 87 (1), 257-272 (2013)
   PUBMED   23077300
REFERENCE   5  (residues 1 to 425)
  AUTHORS   Sugiura T.
  TITLE     The cellular level of TRIM31, an RBCC protein overexpressed in
            gastric cancer, is regulated by multiple mechanisms including the
            ubiquitin-proteasome system
  JOURNAL   Cell Biol. Int. 35 (7), 657-661 (2011)
   PUBMED   21231912
  REMARK    GeneRIF: The cellular level of TRIM31, an RBCC protein
            overexpressed in gastric cancer, is regulated by multiple
            mechanisms including the ubiquitin-proteasome system.
REFERENCE   6  (residues 1 to 425)
  AUTHORS   Sugiura T and Miyamoto K.
  TITLE     Characterization of TRIM31, upregulated in gastric adenocarcinoma,
            as a novel RBCC protein
  JOURNAL   J. Cell. Biochem. 105 (4), 1081-1091 (2008)
   PUBMED   18773414
REFERENCE   7  (residues 1 to 425)
  AUTHORS   Uchil PD, Quinlan BD, Chan WT, Luna JM and Mothes W.
  TITLE     TRIM E3 ligases interfere with early and late stages of the
            retroviral life cycle
  JOURNAL   PLoS Pathog. 4 (2), e16 (2008)
   PUBMED   18248090
REFERENCE   8  (residues 1 to 425)
  AUTHORS   Hillman RT, Green RE and Brenner SE.
  TITLE     An unappreciated role for RNA surveillance
  JOURNAL   Genome Biol. 5 (2), R8 (2004)
   PUBMED   14759258
REFERENCE   9  (residues 1 to 425)
  AUTHORS   Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L,
            Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci
            S, Pelicci PG and Ballabio A.
  TITLE     The tripartite motif family identifies cell compartments
  JOURNAL   EMBO J. 20 (9), 2140-2151 (2001)
   PUBMED   11331580
REFERENCE   10 (residues 1 to 425)
  AUTHORS   el Kahloun A, Chauvel B, Mauvieux V, Dorval I, Jouanolle AM,
            Gicquel I, Le Gall JY and David V.
  TITLE     Localization of seven new genes around the HLA-A locus
  JOURNAL   Hum. Mol. Genet. 2 (1), 55-60 (1993)
   PUBMED   8490624
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG403536.1, BM847963.1,
            AF230386.1 and CB142265.1.
            On Apr 22, 2005 this sequence version replaced NP_008959.2.
            
            Summary: This gene encodes a protein that functions as an E3
            ubiquitin-protein ligase. This gene shows altered expression in
            certain tumors and may be a negative regulator of cell growth.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1, also known as alpha)
            represents the longest transcript and encodes the protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF230386.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.1"
     Protein         1..425
                     /product="E3 ubiquitin-protein ligase TRIM31"
                     /EC_number="2.3.2.27"
                     /note="tripartite motif-containing protein 31; RING-type
                     E3 ubiquitin transferase TRIM31"
                     /calculated_mol_wt=48113
     Region          13..56
                     /region_name="RING-HC_TRIM31_C-V"
                     /note="RING finger, HC subclass, found in tripartite
                     motif-containing protein 31 (TRIM31) and similar proteins;
                     cd16582"
                     /db_xref="CDD:319496"
     Region          16..56
                     /region_name="RING-HC finger (C3HC4-type)"
                     /note="RING-HC finger (C3HC4-type) [structural motif]"
                     /db_xref="CDD:319496"
     Site            order(16,19,31,33,36,39,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:319496"
     Region          90..131
                     /region_name="BBOX"
                     /note="B-Box-type zinc finger; smart00336"
                     /db_xref="CDD:197662"
     Site            order(95,98,117,123)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:237988"
     CDS             1..425
                     /gene="TRIM31"
                     /gene_synonym="C6orf13; HCG1; HCGI; RNF"
                     /coded_by="NM_007028.4:127..1404"
                     /db_xref="CCDS:CCDS34374.1"
                     /db_xref="GeneID:11074"
                     /db_xref="HGNC:HGNC:16289"
                     /db_xref="MIM:609316"
ORIGIN      
        1 masgqfvnkl qeevicpicl dilqkpvtid cghnfclkci tqigetscgf fkcplcktsv
       61 rknairfnsl lrnlvekiqa lqasevqskr keatcprhqe mfhyfceddg kflcfvcres
      121 kdhkshnvsl ieeaaqnyqg qiqeqiqvlq qkeketvqvk aqgvhrvdvf tdqvehekqr
      181 iltefellhq vleeeknfll sriywlgheg teagkhyvas tepqlndlkk lvdslktkqn
      241 mpprqlledi kvvlcrseef qflnptpvpl elekklseak srhdsitgsl kkfkdqlqad
      301 rkkdenrffk smnkndmksw gllqknnhkm nktsepgsss aggrttsgpp nhhssapshs
      361 lfrassakv tfpvcllasy deisgqgass qdtktfdval seelhaalse wltairawfc

      421 evpss

Muistiin 4.4. 2018