https://www.ncbi.nlm.nih.gov/pubmed/28082312
EMBO Rep. 2017 Mar;18(3):392-402. doi: 10.15252/embr.201643205. Epub 2017 Jan 12.
A single MIU motif of MINDY-1 recognizes K48-linked polyubiquitin chains.
The
eight different types of ubiquitin (Ub) chains that can be formed play
important roles in diverse cellular processes. Linkage-selective
recognition of Ub chains by Ub-binding domain (UBD)-containing proteins
is central to coupling different Ub signals to specific cellular
responses. The motif interacting with ubiquitin (MIU)
is a small UBD that has been characterized for its binding to monoUb.
The recently discovered deubiquitinase MINDY-1/FAM63A contains a tandem MIU repeat (tMIU) that is highly selective at binding to K48-linked polyUb. We here identify that this linkage-selective binding is mediated by a single MIU motif (MIU2) in MINDY-1. The crystal structure of MIU2 in complex with K48-linked polyubiquitin chains reveals that MIU2 on its own binds to all three Ub moieties in an open conformation that can only be accommodated by K48-linked triUb. The weak Ub binder MIU1 increases overall affinity of the tMIU for polyUb chains without affecting its linkage selectivity. Our analyses reveal new concepts for linkage selectivity and polyUb recognition by UBDs.
The recently discovered deubiquitinase MINDY-1/FAM63A contains a tandem MIU repeat (tMIU) that is highly selective at binding to K48-linked polyUb. We here identify that this linkage-selective binding is mediated by a single MIU motif (MIU2) in MINDY-1. The crystal structure of MIU2 in complex with K48-linked polyubiquitin chains reveals that MIU2 on its own binds to all three Ub moieties in an open conformation that can only be accommodated by K48-linked triUb. The weak Ub binder MIU1 increases overall affinity of the tMIU for polyUb chains without affecting its linkage selectivity. Our analyses reveal new concepts for linkage selectivity and polyUb recognition by UBDs.
© 2017 The Authors.
KEYWORDS:
MINDY deubiquitinase; motif interacting with ubiquitin; polyubiquitin; ubiquitin binding domain; ubiquitin signaling
MINDY deubiquitinase; motif interacting with ubiquitin; polyubiquitin; ubiquitin binding domain; ubiquitin signaling
PMID: 28082312
PMCID: PMC5331195
DOI: 10.15252/embr.201643205
[Indexed for MEDLINE] Free PMC Article
Myös E3 uikitiiniligaasi RING-finger proteiini 168 sisältää MIU1 ja MIU2- domeenit
PMCID: PMC5331195
DOI: 10.15252/embr.201643205
[Indexed for MEDLINE] Free PMC Article
Myös E3 uikitiiniligaasi RING-finger proteiini 168 sisältää MIU1 ja MIU2- domeenit
Inga kommentarer:
Skicka en kommentar