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tisdag 2 juli 2019

ZIKV ja viruksen SUMO-interaktion tekevä motiivi SIM (vidl tai vvdl )

https://www.ncbi.nlm.nih.gov/pubmed/30658479

2019 Jan 17;20(2). pii: E392. doi: 10.3390/ijms20020392.

Targeting SUMO Modification of the Non-Structural Protein 5 of Zika Virus as a Host-Targeting Antiviral Strategy.

Zhu Z1,2, Chu H3,4, Wen L5, Yuan S6,7, Chik KK8, Yuen TT9, Yip CC10, Wang D11, Zhou J12,13, Yin F14,15,16, Jin DY17, Kok KH18,19, Yuen KY20,21,22,23,24, Chan JF25,26,27,28.

Abstract

Post-translational modifications of host or viral proteins are key strategies exploited by viruses to support virus replication and counteract host immune response. SUMOylation is a post-translational modification process mediated by a family of ubiquitin-like proteins called small ubiquitin-like modifier (SUMO) proteins. Multiple sequence alignment of 78 representative flaviviruses showed that most (72/78, 92.3%) have a putative SUMO-interacting motif (SIM) at their non-structural 5 (NS5) protein's N-terminal domain. The putative SIM was highly conserved among 414 pre-epidemic and epidemic Zika virus (ZIKV) strains, with all of them having a putative SIM core amino acid sequence of VIDL (327/414, 79.0%) or VVDL (87/414, 21.0%). Molecular docking predicted that the hydrophobic SIM core residues bind to the β2 strand of the SUMO-1 protein, and the acidic residues flanking the core strengthen the binding through interactions with the basic surface of the SUMO protein. The SUMO inhibitor 2-D08 significantly reduced replication of flaviviruses and protected cells against ZIKV-induced cytopathic effects in vitro. A SIM-mutated ZIKV NS5 failed to efficiently suppress type I interferon signaling. Overall, these findings may suggest SUMO modification of the viral NS5 protein to be an evolutionarily conserved post-translational modification process among flaviviruses to enhance virus replication and suppress host antiviral response.

KEYWORDS:

NS5; SUMO; Zika; antiviral; flavivirus; inhibitor; interferon; post-translational modification
PMID:
30658479
PMCID:
PMC6359730
DOI:
10.3390/ijms20020392

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