MUC20 (3q29) , MUC-20, corneal, conjunctival mucin-20

https://www.ncbi.nlm.nih.gov/gene/200958

Also known as

MUC-20

Summary

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]

Expression

Broad expression in gall bladder (RPKM 28.1), colon (RPKM 25.9) and 17 other tissues See more

Related articles in PubMed

1. MUC20 promotes aggressive phenotypes of epithelial ovarian cancer cells via activation of the integrin β1 pathway. Chen CH, et al. Gynecol Oncol, 2016 Jan. PMID 26616226
2. Expression analysis of the transmembrane mucin MUC20 in human corneal and conjunctival epithelia. Woodward AM, et al. Invest Ophthalmol Vis Sci, 2014 Aug 28. PMID 25168902, Free PMC Article RESULTS: The MUC20 was detected throughout the entire human ocular surface epithelia, predominantly in cell membranes within intermediate cell layers. In conjunctiva, MUC20 also was observed in the cytoplasm of apical cells within the stratified squamous epithelium, but not in goblet cells. Quantitative PCR and immunoblotting demonstrated expression of MUC20 in HCLE and HCjE cells. Induction of differentiation with serum-containing medium resulted in upregulation of MUC20 mRNA and protein. Biotin labeling of the surface of stratified cultures revealed low levels of MUC20 protein on apical glycocalyces. Further, MUC20 was not detected in the cell culture media or in human tears, suggesting that the extracellular domain of MUC20 is not released from the ocular surface as described previously for other cell surface mucins. CONCLUSIONS: Our results indicate that MUC20 is a novel transmembrane mucin expressed by the human corneal and conjunctival epithelia, and suggest that differential expression of MUC20 during differentiation has a role in maintaining ocular surface homeostasis.
   
   Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
   KEYWORDS:
   MUC20; conjunctiva; cornea; epithelial cell; transmembrane mucin
3. Role of MUC20 overexpression as a predictor of recurrence and poor outcome in colorectal cancer. Xiao X, et al. J Transl Med, 2013 Jun 20. PMID 23787019, Free PMC Article
4. Antitumor T cell responses in bladder cancer are directed against a limited set of antigens and are modulated by regulatory T cells and routine treatment approaches. Horn T, et al. Int J Cancer, 2013 Nov. PMID 23625723
5. MUC20 overexpression predicts poor prognosis and enhances EGF-induced malignant phenotypes via activation of the EGFR-STAT3 pathway in endometrial cancer.

 GeneRif

1. High MUC20 expression is associated wit response to chemotherapy in esophageal squamous cell carcinoma.
2. increased gene expression of MUC16 and MUC20 was found in patients with remission ulcerative colitis
3. A combination of MUC13/MUC20 expression was a potential prognostic marker for patients with ESCC, who received neoadjuvant chemotherapy followed by surgery.
4. Results suggest that MUC20 enhances aggressive behaviors of EOC cells by activating integrin beta1 signaling and provide novel insights into the role of MUC20 in ovarian cancer metastasis.
5. MUC20 is a novel transmembrane mucin expressed by the human corneal and conjunctival epithelia, and suggest that differential expression of MUC20 during differentiation has a role in maintaining ocular surface homeostasis.
6. MUC20 is expressed in recurrent colorectal neoplasms and may have a role in poor outcome
7. Reflux laryngitis is associated with down-regulation of mucin gene expression.
8. Observational study of gene-disease association. (HuGE Navigator)
9. Results suggest that MUC20 is a novel regulator of the Met signaling cascade which has a role in suppression of the Grb2-Ras pathway.

 MUC20  structure:

 Conserved domainPHA3247 large tegument protein UL36 

 https://www.ncbi.nlm.nih.gov/protein/NP_001278762.1

• Analysis of the UL36 open reading frame encoding the large tegument protein (ICP1/2) of herpes simplex virus type 1.J. Virol. 1992 Dec; 66(12):7581-7584
• The UL10 gene of herpes simplex virus 1 encodes a novel viral glycoprotein, gM, which is present in the virion and in the plasma membrane of infected cells.J. Virol. 1993 Mar; 67(3):1441-1452
• A deubiquitinating activity is conserved in the large tegument protein of the herpesviridae.J. Virol. 2005 Dec; 79(24):15582-15585
  STRUCTURE 
   https://www.ncbi.nlm.nih.gov/protein/NP_001269435.1

• Tämä korneaalinen konjunktivaalinen musiin MUC20 ei esiinny kyynelnesteessä. Kyynelnesteen musiini  (lakrimaalinen  muc) on toinen:MUC5AC

J Ophthalmol. 2018 Oct 14;2018:1061276. doi: 10.1155/2018/1061276. eCollection 2018. Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients. Nebbioso M¹, Sacchetti M¹, Bianchi G¹, Zicari AM², Duse M², Del Regno P¹, Lambiase A¹. Abstract Background:

Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose:
To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops.  Methods:
Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops.  Results:
Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions:
The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy. PMID:30405906PMCID:PMC6204206 DOI: 10.1155/2018/1061276

Free PMC Article