Nukleostemiinin ja ubikitiinin suhde

http://www.jbc.org/content/early/2012/02/08/jbc.M111.335141.full.pdf

BACKGROUND: Proteasomal turnover. Nucleostemin (NS) in response to low

cellular GTP levels was previously shown to be MDM2-dependent without clear

mechanisms.

RESULTS: NS is ubiquitinated in cells, but its MPA-induced turnover still occurs

without ubiquitination and MDM2.

CONCLUSION: NS is ubiquitinated in cells, and its turnover is ubiquitin- and

MDM2-independent.

SIGNIFICANCE: NS ubiquitination may be important for regulation of NS function.

Geeni  Kr.3p21.1. G protein nucleolar 3, GNL3
https://www.ncbi.nlm.nih.gov/gene/26354#gene-expression

Also known as

NS; E2IG3; NNP47; C77032

Summary

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Expression

Ubiq

uitous expression in urinary bladder (RPKM 35.8), prostate (RPKM 32.9) and 25 other tissues See more

1. Knockdown of Nucleostemin in an ovarian cancer SKOV-3 cell line and its effects on cell malignancy. Wang J, et al. Biochem Biophys Res Commun, 2017 May 27. PMID 28412352
2. Expression of nucleostemin gene in primary osteoarthritis. Louka ML, et al. Gene, 2016 Aug 1. PMID 27066995
3. Guanine nucleotide binding protein-like 3 is a potential prognosis indicator of gastric cancer. Chen J, et al. Int J Clin Exp Pathol, 2015. PMID 26722529, Free PMC Article
4. Nucleostemin promotes the proliferation of human glioma via Wnt/β-Catenin pathway. Bao Z, et al. Neuropathology, 2016 Jun. PMID 26607678
5. Nucleostemin Knockdown Sensitizes Hepatocellular Carcinoma Cells to Ultraviolet and Serum Starvation-Induced Apoptosis. Yuan F, et al. PLoS One, 2015. PMID 26517370, Free PMC Article  

Gene References Into FunctionsWhat's a GeneRIF?

1. In addition, migration and invasion demonstrated significant inhibition in Nucleostemin-deficient cells. Furthermore, the knockdown of Nucleostemin dramatically suppressed xenograft progression in BALB/c nude mice. Our findings suggest that Nucleostemin is associated with malignancy in an ovarian cancer SKOV-3 cell line.
2. Identified a positive correlation between up-regulation of nucleostemin and human glioma cell proliferation and that knocking-down nucleostemin alleviated glioma proliferation by reducing beta-Catenin transportation into the nucleus.
3. Relative expression of GNL3 in synovial tissue and fluid samples was significantly higher in the osteoarthritic group as compared to the non-osteoarthritic group.
4. The expression level of GNL3 was examined in 89 human gastric cancer samples using immunohistochemistry (IHC) staining. GNL3 in gastric cancer tissues was significantly upregulated compared with paracancerous tissues.
5. Study demonstrated overexpression of NS in most hepatocellular carcinoma tissues compared with their matched surrounding tissues, and silencing NS promoted UV- and serum starvation-induced apoptosis of MHCC97H and Bel7402 cells.
6. over-expression of GNL3 and SKA3 in the PC-3 human prostate cancer cell line decreased in vitro cell migration and invasion
7. p53 activation by inauhzin via inhibiting SIRT1, also inhibits cellular IMPDH2 activity, reduces the levels of cellular GTP and GTP-binding nucleostemin, thus representing in a new anti-cancer strategy.
8. overexpression of NS suppressed ARF polyubiquitination by its E3 ligase Ubiquitin Ligase for ARF and elongated its half-life, whereas knockdown of NS led to the decrease of ARF levels
9. Silence of nucleostemin inhibit cell proliferation through up-regulating p21 expression and nucleostemin may be involved in the pathogenesis of esophageal squamous cell carcinoma.
10. It is concluded that nucleostemin expression could not be specific in a certain type of cells in colon cancer cell line HT29.

Submit: New GeneRIF Correction See all GeneRIFs (55)

Nukleostemiiniproteiini
 Huomaa tässä on paljon K, lysiinejä, paljon myös lysiinipareja KK,  mutta silti se ei mene ubikitiiniproteosomihajoitukseen, vaan ubikitiini suojelee sitä. 
rakenteessa on erilaisia GTP-aasisekvenssejä   Ribosomi biogeneesin GTPaasi A etc
https://www.ncbi.nlm.nih.gov/protein/AAV74413.1
peptidi

     
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  61 lreaelrkqr leelkqqqkl drqkeqekkr kletnpdikp snvepmekef glcktenkak
 121 sgkqnskkly cqelkkviea sdvvlevlda rdplgcrcpq veeaivqsgq kklvlilnks
 181 dlvpkenles wlnylkkelp tvvfrastkp kdkgkitkrv kakknaapfr sevcfgkegl
 241 wkllggfqet cskairvgvi gfpnvgkssi inslkqeqmc nvgvsmgltr smqvvpldkq
 301 itiidspsfi vsplnsssal alrspasiev vkpmeaasai lsqadarqvv lkytvpgyrn
 361 slefftmlaq rrgmhqkggi pnvegaakll wsewtgasla yychpptswt pppyfnesiv
 421 vdmksgfnle elekndaqsi raikgphlan silfqssglt ngiieekdih eelpkrkerk
 481 qeereddkds dqetadeevd enssgmfaae etgealseet tageqstrsf ildkiieedd
 541 aydfstdyv