(4) RNF148 (Kr.7q31.32) , PA-TM-RING RNF.alaryhmä , GRAILin kaltainen domeeni rakenteessa

RNF148 (Kr.7q31.32) (sama kromosomipaikka kuin RNF133:lla).

PA-TM-RING RNF alaryhmä

Related articles in PubMed

1. Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases. Liu YQ, et a
   

• .Genet Mol Res, 2013 Sep 27. PMID 24089095

• Does this have a familiar RING? Saurin AJ, et al. Tren
  

2. s Biochem Sci, 1996 Jun. PMID 8744354
3. Human chromosome 7: DNA sequence and biology. Scherer SW, et al. Science, 2003 May 2. PMID 12690205, Free PMC Article
4. Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039
5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334, Free PMC Article
   

See all (7) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. We identified a novel human testicular interstitial gene and observed that histone deacetylases regulate RNF148 expression. Abstract 2013 .
   
   Suomennosta. Vuonna 2013 julkaistun artikkelin mukaan on tätä geeniä havaittu restriktiivisesti vain ihmisen tekstiksessä ja sellaisilla restriktiogeeneillä tapaa olla tärkeää osuutta spermatogeneesiin. Näitä geenejä selvitellessään tutkijaryhmä tunnisti RNF148 geenin, jota ilmeni runsaasti testiksessä ja hieman myös haimassa. RNF148 mRNA ilmeni interstitiaalikudoksessa ja proteiinia esiintyi samassa sijainnissa. Eräällä histonideasetylaasin (HDAC) estäjällä aktivoitui RFN148 ilmenemä sellaisista soluista, jotka eivät normaalisti ilmennä RNF148 geeniä. Mainitun histonideasetylaasinestäjän osoitettiin (kromatiinin immuunisaostusanalyysissä) lisäävän asetyloituneen hstonin H3 sitoutumista RNF148-geenin promoottoriin Näin tutkijat pystyivät osoittamaan uuden i interstitiaalikudoksen geenin ihmisestäkin ja havaitsivat histonideasetylaasien (HDACs) säätelevän sen ilmenemistä.

• Multiple genes are restrictively expressed in mammalian testicular tissues, and they play important roles in the complex process of spermatogenesis. Investigation of these genes and their expression regulation mechanisms is valuable to elucidate the molecular process of spermatogenesis. In this study, we identified a novel human gene, ring finger protein 148 (RNF148) that is abundantly expressed in testes and slightly expressed in pancreas. In situ hybridization analysis showed that RNF148 messenger RNA was mainly present in the interstitial cells of human testicular tissues, and immunohistochemical analysis confirmed protein levels in that location. Treatment with histone deacetylase inhibitor trichostatin A activated the expression of RNF148 messenger RNA in a time- and concentration-dependent manner in HEK293T and HeLa cells, neither of which normally express RNF148. Chromatin immunoprecipitation analysis showed that trichostatin A treatment increased the binding of acetylated histone H3 to the RNF148 gene promoter. We identified a novel human testicular interstitial gene and observed that histone deacetylases regulate RNF148 expression.

Related articles in PubMed

1. Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases. Liu YQ, et a
   

• . Genet Mol Res, 2013 Sep 27. PMID 24089095

• Does this have a familiar RING? Saurin AJ, et al. Tren
  

2. s Biochem Sci, 1996 Jun. PMID 8744354
3. Human chromosome 7: DNA sequence and biology. Scherer SW, et al. Science, 2003 May 2. PMID 12690205, Free PMC Article
4. Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039
5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334, Free PMC Article
   

See all (7) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. We identified a novel human testicular interstitial gene and observed that histone deacetylases regulate RNF148 expression.
   

Conserved Domains (2) summary

cd02122
Location:43 → 177

PA_GRAIL_like; PA _GRAIL_like: Protease-associated (PA) domain GRAIL-like. This group includes PA domain containing E3 (ubiquitin ligases) similar to human GRAIL (gene related to anergy in lymphocytes) protein. Proteins in this group contain a C3H2C3 RING finger. E3 ...

pfam13639
Location:256 → 299

zf-RING_2; Ring finger domain

Peptide history and sequence : Precursor protein 1..305.

https://www.ncbi.nlm.nih.gov/protein/NP_932351.1