Ubikitiinin de novo synteesi . UBA80 (2p16.1) , RPS27A , Monoubikitiini, fuusioproteiini, Solustressisensori

( Ubikitiini A laji )Tästä geenistä on aika runsaasti merkitsevää kliinistä  tietoa. Näyttää siltä että tätä ubikitiinilaatua syntyy  solustressin ja DNA vaurion  indusoimana p53:n toimesta.
 Tämä ubikitiinigeeni koodaa fuusioproteiinia, jonka N-terminaalissa on yksi ubikitiini-alue ja C-terminaalissa ribosomaalinen proteiini S27a. Hiivassa  tämä konservoitunut geeni tuottaa vapaan  monoubikitiinin ja ribosomaalisen proteiinin  S27a. (  S27a on komponettina  ribosomin 40S alayksikössä). Tässä prekursoriproteiinissa on useita  asetyloituneita lysiinejä C-terminaalisessa jaksossa. Kuvassa ylin on  tämän UBA80 kaava.

https://www.researchgate.net/profile/Choongseob_Oh/publication/256489807/figure/fig1/AS:213970884534276@1428026042193/a-Schematic-representation-of-the-4Ub-genes-Rps27a-and-Uba52-encode-Ub-as-a-fusion.png

PubMeD  lähde käyttää mieluiten geeninimeä RPS27A, ( mikä on ymmärrettävää, että  ei tule sekaannusta, koska  tässä järejstelmsäsä on tosiaan paljon  näitä UB nimiä) 
https://www.ncbi.nlm.nih.gov/gene/6233

also known as

UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80

Summary

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.

 This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. 

When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm.

 Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]

Expression

Ubiquitous expression in ovary (RPKM 575.2), lymph node (RPKM 347.6) and 25 other tissues See more

Orthologs

mouse all

Preferred Names

ubiquitin-40S ribosomal protein S27a

Names

40S ribosomal protein S27a

epididymis luminal protein 112

ubiquitin C

ubiquitin and ribosomal protein S27a

ubiquitin carboxyl extension protein 80

ubiquitin-CEP80

Conserved Domains (2) summary

cd01803
Location:1 → 76

Ubiquitin; Ubiquitin

pfam01599
Location:103 → 147

Ribosomal_S27; Ribosomal protein S27a

 Peptide history, structure: precursor peptide
 https://www.ncbi.nlm.nih.gov/protein/NP_001129064.1

Related articles in PubMed

1. Identification and expression of MMSA-8, and its clinical significance in multiple myeloma. He R, et al. Oncol Rep, 2017 Jun. PMID 28498418, Free PMC Article
2. RPS27a promotes proliferation, regulates cell cycle progression and inhibits apoptosis of leukemia cells. Wang H, et al. Biochem Biophys Res Commun, 2014 Apr 18. PMID 24680683
3. A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer. Ko Y, et al. Int J Mol Med, 2005 Feb. PMID 15647830
4. Structure and properties of a dimeric N-terminal fragment of human ubiquitin. Bolton D, et al. J Mol Biol, 2001 Dec 7. PMID 11733996
5. Identification of the long ubiquitin extension as ribosomal protein S27a. Redman KL, et al. Nature, 1989 Mar 30. PMID 2538756

See all (108) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

1. the full-length cDNA sequence of MMSA-8 was cloned in MM and it was hypothesized that MMSA-8 is MM-associated RPS27A transcript variant 1
2. RPS27a enhances viral LMP1-mediated proliferation and invasion, suggesting that RPS27a interacts with LMP1 and stabilizes it by suppressing proteasome-mediated ubiquitination.
3. Imatinib-resistant K562/G01 cells expressed significantly higher levels of STAT3 and RPS27a compared with those of K562 cells.
4. RPS27A expression was found to have a weak inverse correlation with overexpression of multifunctional protein YB-1 in HCC tissues.
5. RPS27a appears to be a novel stress sensor in the cell which amplifies p53 response to arrest cell cycle....The small ribosomal protein RPS27a is known to play a role in the activation of cellular checkpoints via p53 which links ribosome biogenesis to cell cycle progression. Here, we show that RPS27a gene is a direct transcriptional target of p53 and is overexpressed in response to DNA damage. Elevated RPS27a level was associated with increased expression of p53 and its target p21(Waf1) gene. The RPS27a activity was specifically inhibited in the presence of a dominant negative mutant of p53. Down-regulation of ectopically expressed RPS27a by RNA interference blocked the activation of p21(waf1) in response to DNA damage. Thus, RPS27a appears to be a novel stress sensor in the cell which amplifies p53 response to arrest cell cycle.
6. Knockdown of RPS27a inhibits the proliferation, induces cell cycle arrest and potentiates the effect of imatinib on apoptosis of K562 cells.
7. Gene expression profiles showed that RPS27A was down-regulated in epidermolysis bullosa subtypes.
8. S27a plays a non-redundant role in mediating p53 activation in response to ribosomal stress via interplaying with MDM2.
9. ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer