KLHL21(1p36.31), kriittinen mitoosin säätelijä , sytokineesi, Aurora B rekrytointi

https://www.ncbi.nlm.nih.gov/pubmed/19995937/
 KLHL21 (1p36.31)

Expression

Ubiquitous expression in fat (RPKM 17.4), ovary (RPKM 14.2) and 25 other tissues See more

Orthologs

mouse all

1. Inhibition of KLHL21 prevents cholangiocarcinoma progression through regulating cell proliferation and motility, arresting cell cycle and reducing Erk activation. Chen J, et al. Biochem Biophys Res Commun, 2018 May 15. PMID 29574153
2. KLHL21, a novel gene that contributes to the progression of hepatocellular carcinoma. Shi L, et al. BMC Cancer, 2016 Oct 21. PMID 27769251, Free PMC Article
3. Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase. Courtheoux T, et al. Nat Commun, 2016 Sep 19. PMID 27641145, Free PMC Article
4. Kelch-like Protein 21 (KLHL21) Targets IκB Kinase-β to Regulate Nuclear Factor κ-Light Chain Enhancer of Activated B Cells (NF-κB) Signaling Negatively. Mei ZZ, et al. J Biol Chem, 2016 Aug 26. PMID 27387502, Free PMC Article
5. Ubiquitylation-dependent localization of PLK1 in mitosis. Beck J, et al. Nat Cell Biol, 2013 Apr. PMID 23455478

See all (19) citations in PubMed

J Cell Biol. 2009 Dec 14;187(6):791-800. doi: 10.1083/jcb.200906117.

The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis.
Abstract

Cul3 (Cullin3)-based E3 ubiquitin ligases recently emerged as critical regulators of mitosis. In this study, we identify two mammalian BTB (Bric-a-brac-Tramtrack-Broad complex)-Kelch proteins, KLHL21 and KLHL22, that interact with Cul3 and are required for efficient chromosome alignment. Interestingly, KLHL21 but not KLHL22 is necessary for cytokinesis and regulates translocation of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase, similar to the previously described BTB-Kelch proteins KLHL9 and KLHL13. KLHL21 directly binds to aurora B and mediates ubiquitination of aurora B in vitro. In contrast to KLHL9 and KLHL13, KLHL21 localizes to midzone microtubules in anaphase and recruits aurora B and Cul3 to this region. Together, our results suggest that different Cul3 adaptors nonredundantly regulate aurora B during mitosis, possibly by ubiquitinating different pools of aurora B at distinct subcellular localizations.

PMID:

19995937

PMCID:

PMC2806313

DOI:

10.1083/jcb.200906117