KLHL18 (3p21.31). sentrosomaalisen Aurora-A kinaasin aktivaatio, mitoosialun ajoitus

https://www.ncbi.nlm.nih.gov/gene/23276

Expression

Ubiquitous expression in bone marrow (RPKM 6.1), skin (RPKM 5.8) and 25 other tissues See more

1. The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A. Moghe S, et al. Biol Open, 2012 Feb 15. PMID 23213400, Free PMC Article   The cullin-RING family of ubiquitin ligases regulates diverse cellular functions, such as cell cycle control, via ubiquitylation of specific substrates. CUL3 targets its substrates through BTB proteins. Here we show that depletion of CUL3 and the BTB protein KLHL18 causes a delay in mitotic entry. Centrosomal activation of Aurora-A, a kinase whose activity is required for entry into mitosis, is also delayed in depleted cells. Moreover, we identify Aurora-A as a KLHL18-interacting partner. Overexpression of KLHL18 and CUL3 promotes Aurora-A ubiquitylation in vivo, and the CUL3-KLHL18-ROC1 ligase ubiquitylates Aurora-A in vitro. Our study reveals that the CUL3-KLHL18 ligase is required for timely entry into mitosis, as well as for the activation of Aurora-A at centrosomes. We propose that the CUL3-KLHL18 ligase regulates mitotic entry through an Aurora-A-dependent pathway.
2. Update on the Kelch-like (KLHL) gene family. Dhanoa BS, et al. Hum Genomics, 2013 May 15. PMID 23676014, Free PMC Article
3. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. Olma MH, et al. J Cell Sci, 2009 Apr 1. PMID 19295130, Free PMC Article.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720933/figure/fig2/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720933/figure/fig2/
4. Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. Nagase T, et al. DNA Res, 1998 Oct 30. PMID 9872452
5. Mapping the interactome of HPV E6 and E7 oncoproteins with the ubiquitin-proteasome system. Poirson J, et al. FEBS J, 2017 Oct. PMID 28786561

See all (22) citations in PubMed

Search results: KLHL18:

Items: 4

Select item 315173101.

Detection of de novo genetic variants in Mayer-Rokitansky-Küster-Hauser syndrome by whole genome sequencing.

Pan HX, Luo GN, Wan SQ, Qin CL, Tang J, Zhang M, Du M, Xu KK, Shi JQ.

Eur J Obstet Gynecol Reprod Biol X. 2019 Aug 2;4:100089. doi: 10.1016/j.eurox.2019.100089. eCollection 2019 Oct.

PMID:

31517310

Free PMC Article

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Select item 232134002.

The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

Moghe S, Jiang F, Miura Y, Cerny RL, Tsai MY, Furukawa M.

Biol Open. 2012 Feb 15;1(2):82-91. doi: 10.1242/bio.2011018. Epub 2011 Nov 3.

PMID:

23213400

Free PMC Article

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Select item 196981103.

The mRNA expression of SETD2 in human breast cancer: correlation with clinico-pathological parameters.

Al Sarakbi W, Sasi W, Jiang WG, Roberts T, Newbold RF, Mokbel K.

BMC Cancer. 2009 Aug 21;9:290. doi: 10.1186/1471-2407-9-290.

PMID:

19698110

Free PMC Article

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Select item 196247544.

BTB protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex.

Fujiyama-Nakamura S, Ito S, Sawatsubashi S, Yamauchi Y, Suzuki E, Tanabe M, Kimura S, Murata T, Isobe T, Takeyama K, Kato S.

Genes Cells. 2009 Aug;14(8):965-73. doi: 10.1111/j.1365-2443.2009.01323.x. Epub 2009 Jul 14.

PMID:

19624754

Free Article

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