RING2 (1q25.3)

https://www.ncbi.nlm.nih.gov/pubmed/20800574

Biochem Biophys Res Commun. 2010 Sep 24;400(3):389-95. doi: 10.1016/j.bbrc.2010.08.082. Epub 2010 Aug 26.

Regulation of the Polycomb protein RING1B ubiquitination by USP7 (DUB) .

de Bie P¹, Zaaroor-Regev D, Ciechanover A.

Abstract

The E3 ubiquitin ligase RING1B plays an important role in Polycomb-mediated gene silencing by monoubiquitinating histone H2A. Both the activity and stability of RING1B are controlled by ubiquitination in two distinct manners. Self ubiquitination of RING1B generates K6, K27 and K48-based mixed polyubiquitin chain, and is required for its activity as a ligase. On the other hand, its proteasomal degradation is mediated by another ligase; E6-AP catalyzes the formation of K48-based chains. Since these two modes of ubiquitination target the same lysine residues and are therefore mutually exclusive, an important mode of regulation of RING1B should be at the level of deubiquitination. Here we identify USP7 as a deubiquitinating enzyme that regulates the ubiquitination state of RING1B. RING1B interacts with USP7, which is mediated in part by its RING domain. In addition, USP7 was found in a complex with other Polycomb proteins, suggesting a broad role in regulating these complexes. Although, USP7 directly and specifically deubiquitinates RING1B in vitro and in vivo, it does not discriminate between the activating and proteolysis-targeting modes of ubiquitination, and therefore has a stabilizing effect on RING1B.

Copyright © 2010 Elsevier Inc. All rights reserved.

PMID:

20800574

DOI:

10.1016/j.bbrc.2010.08.082


Geeni  RING2 (1q25.3)
 https://www.ncbi.nlm.nih.gov/gene/6045

Also known as

BAP1; DING; BAP-1; HIPI3; RING2; RING1B

Summary

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008]

Expression

Ubiquitous expression in testis (RPKM 7.8), placenta (RPKM 6.0) and 25 other tissues See more

Orthologs

mouse all

 

Preferred Names

E3 ubiquitin-protein ligase RING2

Names

HIP2-interacting protein 3

RING finger protein 1B

RING finger protein BAP-1

RING-type E3 ubiquitin transferase RING2

huntingtin-interacting protein 2-interacting protein 3

protein DinG