UCHL3 (13q22.2), UCH tyyppinen DUB, joka säätelee JAMM- tyyppisen JAB1:n ( COPS5) deneddylaatiota

 

 https://www.ncbi.nlm.nih.gov/gene/7347

Also known as

UCH-L3

Summary

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Expression

Ubiquitous expression in thyroid (RPKM 5.1), testis (RPKM 4.9) and 25 other tissues See more

Orthologs

mouse all

Preferred Names

ubiquitin carboxyl-terminal hydrolase isozyme L3

Names

testicular tissue protein Li 221

ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

ubiquitin thioesterase L3

ubiquitin thiolesterase

Related articles in PubMed

1. Ubiquitin Carboxy-Terminal HydrolaseL3 Correlates with Human Sperm Count, Motility and Fertilization. Wang M, et al. PLoS One, 2016. PMID 27780264, Free PMC Article
2. Characterizing substrate selectivity of ubiquitin C-terminal hydrolase-L3 using engineered α-linked ubiquitin substrates. Navarro MF, et al. Biochemistry, 2014 Dec 30. PMID 25369561
3. Ubiquitin C-terminal hydrolase-L3 regulates EMT process and cancer metastasis in prostate cell lines. Song HM, et al. Biochem Biophys Res Commun, 2014 Sep 26. PMID 25194810
4. A novel RNAi lethality rescue screen to identify regulators of adipogenesis. van Beekum O, et al. PLoS One, 2012. PMID 22679485, Free PMC Article
5. Chalcone-based small-molecule inhibitors attenuate malignant phenotype via targeting deubiquitinating enzymes. Issaenko OA, et al. Cell Cycle, 2012 May 1. PMID 22510564, Free PMC Article

See all (63) citations in PubMed

 The authors find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF(beta-TrCP) complex and associated with SCF(beta-TrCP) activities.
The authors further demonstrate that UCHL3 upregulates the levels of SCF(beta-TrCP) substrates including IFN-I receptor IFNAR1, which enhances IFN-I mediated signaling pathway and antiviral activity. Study demonstrated positive correlations between the level and activity of UCHL3 and sperm characteristics and function suggesting that UCHL3 may play a role in male infertility.
 Using a series of engineered protein substrates, which are similar in size yet differ in secondary structure, we demonstrate that thermal stability is a key factor that significantly affects UCH-L3 hydrolysis.
 UCH-L3 is a novel regulator of epithelial-mesenchymal transition (ENT) and cell migration in prostate cancer cells.
 Data identified of UCHL3 as an essential deubiquitinase in adipogenesis. impaired UCH-L3 function may contribute to the accumulation of full length UBB(+1) in various pathologie identification and the sequence-specific assignments for 186 out of the 218 UCH-L3 (230 less 12 prolines) backbone 15N and amide proton resonances
These results indicate that mono-Ub and Ub dimers may regulate the enzymatic functions of UCH-L1 and UCH-L3, respectively, in vivo.
 Untangling the folding mechanism of the 5(2)-knotted protein UCH-L3
 Observational study of gene-disease association. (HuGE Navigator)