https://www.nature.com/articles/srep12836
Siihen aikaan keskityin varsinaiseti ubikitiiniin itseensä , sen syntymiseen, muta silloin en tarkemmin kuvannut deubikitinaaseja DUB. DUB- asia minulla on vasta nyt menossa. DUB deubikitinaasit ovat tärkeitä pitämässä ubikitiinin säästöä yllä ja vastaa ubikitiinialtaasta, josa on monoubikitiinejä inaktiivina.
Palautan tämän artikkelein tähän:
https://www.nature.com/articles/srep12836
https://www.ncbi.nlm.nih.gov/pubmed/26235645
Abstract
Protein
ubiquitination, a major post-translational modification in eukaryotes,
requires an adequate pool of free ubiquitin. Cells maintain this pool by
two pathways, both involving deubiquitinases (DUBs): recycling of
ubiquitin from ubiquitin conjugates and processing of ubiquitin
precursors synthesized de novo. Although many advances have been
made in recent years regarding ubiquitin recycling, our knowledge on
ubiquitin precursor processing is still limited, and questions such as
when are these precursors processed and which DUBs are involved remain
largely unanswered. Here we provide data suggesting that two of the four
mammalian ubiquitin precursors, UBA52 and UBA80, are processed mostly
post-translationally whereas the other two, UBB and UBC, probably
undergo a combination of co- and post-translational processing.
Using an unbiased biochemical approach we found that UCHL3, USP9X, USP7, USP5 and Otulin/Gumby/FAM105b are by far the most active DUBs acting on these precursors.
The identification of these DUBs together with their properties suggests that each ubiquitin precursor can be processed in at least two different manners, explaining the robustness of the ubiquitin de novo synthesis pathway.
2.
Using an unbiased biochemical approach we found that UCHL3, USP9X, USP7, USP5 and Otulin/Gumby/FAM105b are by far the most active DUBs acting on these precursors.
The identification of these DUBs together with their properties suggests that each ubiquitin precursor can be processed in at least two different manners, explaining the robustness of the ubiquitin de novo synthesis pathway.
2.
Inga kommentarer:
Skicka en kommentar