https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0101532
Abstract
Background
The
prevention and treatment of Microwave-caused cardiovascular injury
remains elusive. This study investigated the cardiovascular protective
effects of compound Chinese medicine “Kang Fu Ling” (KFL) against high
power microwave (HPM)-induced myocardial injury and the role of the
mitochondrial permeability transition pore (mPTP) opening in KFL
protection.
Introduction
With the advent of wireless technology, there has been a massive increase of electromagnetic radiation exposure to human beings from microwaves to radio waves and other invisible radiation. It has been known that certain intensities of microwave radiation, especially high power microwave (HPM), could damage multiple organs, including heart [1]–[10]. ( average HPM power density 30 mW/cm2 for 15 min)
However, the prevention and treatment of HPM-caused cardiovascular injury remains elusive. In recent years, the favorable preclinical results have been obtained with some natural compounds from traditional Chinese medicine [8], [11]. Towards that end, there is an urgent need to gain insights into the molecular basis of their effects and develop novel therapies for microwave-radiation–related myocardial injury.
In this study, we focused on “Kang Fu Ling” (KFL), a compound isolated from Chinese medicine astragalus, red peony, salvia, ophelia, and wolfberry. By using a rat microwave radiation model, we showed that KFL has myocardial protective effects by electrophysiology testing and histological analysis. Further investigation of molecular mechanisms by which pathogenesis is prevented by KFL revealed prevention of mitochondrial dysfunction may be the key event involved in KFL’s myocardial protection effects.
The increased risk of damage to the mitochondria is associated with the massive opening of mitochondrial permeability transition pore (mPTP) under pathological conditions of mitochondrial calcium overload [12]–[14]. We have found previously that KFL significantly decreased calcium level and activities of serum myocardial enzymes, such as creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST), etc, after microwave radiation [15]. Thus, we hypothesized now that the inhibition of mPTP opening by KFL may be responsible for its myocardial protection effects from HPM-induced myocardial injury. Indeed, in this study we found that changes of several mPTP associated protein expression after microwave radiation could be reverted by KFL treatment. This study sheds new light on the role of mPTP in mitochondrial dysfunction and molecular basis of KFL protection.
Introduction
With the advent of wireless technology, there has been a massive increase of electromagnetic radiation exposure to human beings from microwaves to radio waves and other invisible radiation. It has been known that certain intensities of microwave radiation, especially high power microwave (HPM), could damage multiple organs, including heart [1]–[10]. ( average HPM power density 30 mW/cm2 for 15 min)
However, the prevention and treatment of HPM-caused cardiovascular injury remains elusive. In recent years, the favorable preclinical results have been obtained with some natural compounds from traditional Chinese medicine [8], [11]. Towards that end, there is an urgent need to gain insights into the molecular basis of their effects and develop novel therapies for microwave-radiation–related myocardial injury.
In this study, we focused on “Kang Fu Ling” (KFL), a compound isolated from Chinese medicine astragalus, red peony, salvia, ophelia, and wolfberry. By using a rat microwave radiation model, we showed that KFL has myocardial protective effects by electrophysiology testing and histological analysis. Further investigation of molecular mechanisms by which pathogenesis is prevented by KFL revealed prevention of mitochondrial dysfunction may be the key event involved in KFL’s myocardial protection effects.
The increased risk of damage to the mitochondria is associated with the massive opening of mitochondrial permeability transition pore (mPTP) under pathological conditions of mitochondrial calcium overload [12]–[14]. We have found previously that KFL significantly decreased calcium level and activities of serum myocardial enzymes, such as creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST), etc, after microwave radiation [15]. Thus, we hypothesized now that the inhibition of mPTP opening by KFL may be responsible for its myocardial protection effects from HPM-induced myocardial injury. Indeed, in this study we found that changes of several mPTP associated protein expression after microwave radiation could be reverted by KFL treatment. This study sheds new light on the role of mPTP in mitochondrial dysfunction and molecular basis of KFL protection.
Materials and Methods..
..
The amplitude of T waves remained unchanged. These observations suggest that microwave radiation can induce electrophysiological dysfunction of the heart, characterized by reduced heart rate and increased J point shift..
..
The amplitude of T waves remained unchanged. These observations suggest that microwave radiation can induce electrophysiological dysfunction of the heart, characterized by reduced heart rate and increased J point shift..
Inga kommentarer:
Skicka en kommentar