High-Power-Microwave ja solututkimus

https://www.ncbi.nlm.nih.gov/pubmed/17241548
Tässä työssä sädetettiin  vahvatehoisella  mikroaaltosäteilyllä koe-eläimen hypothalamisia neuroneja ja tutkittiin neuronin  tuhoutumismekanismia. HPM säteilyannokset olivat  10 mW/cm(2) ja 30 mW/cm(2). Selvitettiin soluvaurio, mitokondrian kalvopotentiaalin muutos ja kalsiumin pääsy sytoplasmaan. Apoptoosi on päätie hypotalamisen neuronin kuolemassa. HPM- säteilyannoksen nousu lisäsi  nekrotisoitumisen osuutta. Mitokondrian kalvopotentiaali aleni ja kalsiumia pääsi sytoplasmaan ja nää tekijät  vaikutavat neuronin kuoleman prosessiin.

•  Abstract OBJECTIVE:

To explore the injury effect and mechanism of hypothalamic neurons after high power microwave (HPM) exposure. METHODS:
Primarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm(2) HPM, and the inverted phase contrast microscope (IPCM) and flow cytometry (FCM) were employed to detect the injury of cells and change of mitochondrion membrane potential (MMP) and Ca(2+) in the cytoplasm of neurons. RESULTS:
The ratio of apoptosis was significantly higher than that of the sham exposure (P < 0.05) induced by 10 and 30 mW/cm(2) HPM and necrosis increased significantly (P < 0.05) in the group of 30 mW/cm(2) at 6 h after exposure. The content of Ca(2+) in the cytoplasm of neuron cells increased (P < 0.01) while MMP decreased significantly (P < 0.01) after radiation of 30 mW/cm(2) HPM at 6 h after exposure. CONCLUSION:
Apoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca(2+) and the decline of MMP are involved in the process.

PMID:

17241548

[Indexed for MEDLINE]