ZBTB5 (9p13.2); p21 transkirption vaimentaja

  https://www.ncbi.nlm.nih.gov/gene/9925

1. Expression

   Ubiquitous expression in thyroid (RPKM 6.3), esophagus (RPKM 6.3) and 25 other tissues

2. NM_014872.3 → NP_055687.1  zinc finger and BTB domain-containing protein 5
   
   

   Conserved Domains (4) summary

   smart00225
   Location:25 → 119

   BTB; Broad-Complex, Tramtrack and Bric a brac

   sd00017
   Location:615 → 635

   ZF_C2H2; C2H2 Zn finger [structural motif]

   pfam00651
   Location:14 → 119

   BTB; BTB/POZ domain

   pfam13465
   Location:627 → 652

   zf-H2C2_2; Zinc-finger double domain

ORIGIN      
        1 mdfpghfeqi fqqlnyqrlh gqlcdcvivv gnrhfkahrs vlaacsthfr alfsvaegdq
       61 tmnmiqldse vvtaeafaal idmmytstlm lgesnvmdvl laashlhlns vvkackhylt
      121 trtlpmspps ervqeqsarm qrsfmlqqlg lsivssalns sqngeeqpap msssmrsnld
      181 qrtpfpmrrl hkrkqsaeer arqrlrpsid esaisdvtpe ngpsgvhsre effspdslki
      241 vdnpkadgmt dnqedsaimf dqsfgtqeda qvpsqsdnsa gnmaqlsmas ratqvetsfd
      301 qeaapekssf qcenpevglg ekehmrvvvk seplsspepq devsdvtsqa egsesveveg
      361 vvvsaekidl spessdrsfs dpqsstdrvg dihilevtnn lehkstfsis nflnksrgnn
      421 ftanqnnddn ipnttsdcrl eseapyllsp eagpaggpss apgshvenpf sepadshfvr
      481 pmqevmglpc vqtsgyqgge qfgmdfsrsg lglhssfsrv migsprggas nfpyyrriap
      541 kmpvvtsvrs sqipenstss qlmmngatss fenghpsqpg ppqltrasad vlskckkals
      601 ehnvlvvega rkyackicck tfltltdckk hirvhtgekp yaclkcgkrf sqsshlykhs
      661 kttclrwqss nlpstll

Related articles in PubMed

1. A novel POK family transcription factor, ZBTB5, represses transcription of p21CIP1 gene. Koh DI, et al. J Biol Chem, 2009 Jul 24. PMID 19491398, Free PMC Article Abstract
   Transcriptional repression through chromatin remodeling and histone deacetylation has been postulated as a driving force for tumorigenesis. We isolated and characterized a novel POZ domain Krüppel-like zinc finger transcription repressor, ZBTB5 (zinc finger and BTB domain-containing 5). Serial analysis of gene expression (SAGE) analysis showed that ZBTB5 expression is higher in retinoblastoma and muscle cancer tissues. Immunocytochemistry showed that ZBTB5 was localized to the nucleus, particularly nuclear speckles. ZBTB5 directly repressed transcription of cell cycle arrest gene p21 by binding to the proximal GC-box 5/6 elements and the two distal p53-responsive elements (bp -2323 approximately -2299; bp -1416 approximately -1392). Chromatin immunoprecipitation assays showed that ZBTB5 and p53 competed with each other in occupying the p53 binding elements. ZBTB5 interacted with co-repressor-histone deacetylase complexes such as BCoR (BCL-6-interacting corepressor), NCoR (nuclear receptor corepressor), and SMRT (silencing mediator for retinoid and thyroid receptors) via its POZ domain. These interactions resulted in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter, which is important in the transcriptional repression of p21. MTT (3-(4,5-di meth yl thi azol-2-yl)-2,5-diphenyltetrazolium bromide) assays and fluorescent-activated cell sorter analysis revealed that ZBTB5 stimulated both cell proliferation and cell cycle progression, significantly increasing the number of cells in S-phase. Overall, our data suggest that ZBTB5 is a potent transcription repressor of cell cycle arrest gene p21 and a potential proto-oncogene stimulating cell proliferation.

See all (18) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

 ZBTB5 directly repressed transcription of cell cycle arrest gene p21 by binding to the proximal GC-box 5/6 elements and the two distal p53-responsive elements (bp -2323 to -2299; bp -1416 to -1392)