Tälle proteiinille on tyypillsitä useampi DSRM- motiivi ( dsRNA:ta sitova motiivi) .
Vuonna 1996 on julkaistu artikkeli tätä proteiinia koodaavan geenin sijoittumisesta kromosomiin 20q13.3. Geenin nimi STAU. ( Jo Drosophilass on staufin- geeni).
Vuonna 1999 tiedetään, että Ihmesen staufen-sekvenssi on RNA:ta sitova proteiini, ja se assosioituu polysomeihin ja sijoittautuu karkeaan endoplasmiseen retikulumiin (RER). Samana vuonna havaitaan että imettäväisillä staufen liittuu tubuliiniin. Ihmisellä staufen tekee interaktion influenssaviruksen NS1-proteiinin kanssa koeputkessa ja in vivo.
Vuonna 2000 on saatu oivalluksia mRNA-kuljetuksesta ja paikallisesta translaatiosta imettäväisten hermojärjestelmässä.
On huomattu, että SNHG5 stabiloi kohdetranskriptejä blokeeraamalla niiden hajoittamisen STAU1:llä . Sen mukaan STAU1- depleetio palauttaa apoptoosin, jonka on indusoinut SNHG5 poistogeenisyys. Täten SNHG5 luonnehditaan lncRNA:ksi, joka edisiää tuumorisolun elossapysymistä kolorektaalisyövässä.
Vuonna 2016 artikkeli: SNHG5 edistää kolorektaalisyöpäsolun elossapysymistä vastavaikuttamalla STAU-1 välitteiseen mRNA:n destabilisoimiseen.
Vuonna 2017: Influenssaproteiini-interaktomeista tunnistetaan plakofiliini 2:n osuus virusrestriktiossa . Samana vuonna todetaan , että ADAR1 kontrolloi stressattujen solujen apoptoosia estämällä Staufen1- välitteisen mRNA- hajoituksen ( ADAR1p110 isoformi estää kompetitiivisesti Staufen1:n sitoutumisen dsRNA:n 3´UTR-alueeseen ja vastavaikuttaa Staufen1- välitteiseen mRNA:n hävittämiseen).
E2F1 indusoi TINCR transkriptaalisen aktiivisuuden ja kiihdyttää mahasyövän progredioitumista aktivoimalla TINCR/STAU1/CDKN2B-signalointiakselin.
lncRNA Chronos on ikääntymisen indusoima lihashypertrofian estäjä
Sitaatti STAU1 isoformi a rakenteesta:
double-stranded RNA-binding protein Staufen homolog 1 isoform a [Homo sapiens]
NCBI Reference Sequence: NP_004593.2
LOCUS NP_004593 496 aa linear PRI 24-SEP-2018 DEFINITION double-stranded RNA-binding protein Staufen homolog 1 isoform a [Homo sapiens]. ACCESSION NP_004593 VERSION NP_004593.2 DBSOURCE REFSEQ: accession NM_004602.3 KEYWORDS RefSeq. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (residues 1 to 496) AUTHORS Neppl RL, Wu CL and Walsh K. TITLE lncRNA Chronos is an aging-induced inhibitor of muscle hypertrophy JOURNAL J. Cell Biol. 216 (11), 3497-3507 (2017) PUBMED 28855249 REFERENCE 2 (residues 1 to 496) AUTHORS Xu TP, Wang YF, Xiong WL, Ma P, Wang WY, Chen WM, Huang MD, Xia R, Wang R, Zhang EB, Liu YW, De W and Shu YQ. TITLE E2F1 induces TINCR transcriptional activity and accelerates gastric cancer progression via activation of TINCR/STAU1/CDKN2B signaling axis JOURNAL Cell Death Dis 8 (6), e2837 (2017) PUBMED 28569791 REMARK GeneRIF: E2F1 induces TINCR transcriptional activity and accelerates gastric cancer progression via activation of TINCR/STAU1/CDKN2B signaling axis. Publication Status: Online-Only REFERENCE 3 (residues 1 to 496) AUTHORS Sakurai M, Shiromoto Y, Ota H, Song C, Kossenkov AV, Wickramasinghe J, Showe LC, Skordalakes E, Tang HY, Speicher DW and Nishikura K. TITLE ADAR1 controls apoptosis of stressed cells by inhibiting Staufen1-mediated mRNA decay JOURNAL Nat. Struct. Mol. Biol. 24 (6), 534-543 (2017) PUBMED 28436945 REMARK GeneRIF: ADAR1p110 isoform competitively inhibits binding of Staufen1 to the 3'-untranslated-region dsRNAs and antagonizes Staufen1-mediated mRNA decay. REFERENCE 4 (residues 1 to 496) AUTHORS Wang L, Fu B, Li W, Patil G, Liu L, Dorf ME and Li S. TITLE Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction JOURNAL Nat Commun 8, 13876 (2017) PUBMED 28169297 REMARK Publication Status: Online-Only REFERENCE 5 (residues 1 to 496) AUTHORS Damas ND, Marcatti M, Come C, Christensen LL, Nielsen MM, Baumgartner R, Gylling HM, Maglieri G, Rundsten CF, Seemann SE, Rapin N, Thezenas S, Vang S, Orntoft T, Andersen CL, Pedersen JS and Lund AH. TITLE SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization JOURNAL Nat Commun 7, 13875 (2016) PUBMED 28004750 REMARK GeneRIF: Mechanistically, we suggest that SNHG5 stabilizes the target transcripts by blocking their degradation by STAU1. Accordingly, depletion of STAU1 rescues the apoptosis induced after SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting tumour cell survival in colorectal cancer. Publication Status: Online-Only REFERENCE 6 (residues 1 to 496) AUTHORS Kiebler MA and DesGroseillers L. TITLE Molecular insights into mRNA transport and local translation in the mammalian nervous system JOURNAL Neuron 25 (1), 19-28 (2000) PUBMED 10707969 REMARK Review article REFERENCE 7 (residues 1 to 496) AUTHORS Falcon AM, Fortes P, Marion RM, Beloso A and Ortin J. TITLE Interaction of influenza virus NS1 protein and the human homologue of Staufen in vivo and in vitro JOURNAL Nucleic Acids Res. 27 (11), 2241-2247 (1999) PUBMED 10325410 REFERENCE 8 (residues 1 to 496) AUTHORS Wickham L, Duchaine T, Luo M, Nabi IR and DesGroseillers L. TITLE Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum JOURNAL Mol. Cell. Biol. 19 (3), 2220-2230 (1999) PUBMED 10022909 REFERENCE 9 (residues 1 to 496) AUTHORS Marion RM, Fortes P, Beloso A, Dotti C and Ortin J. TITLE A human sequence homologue of Staufen is an RNA-binding protein that is associated with polysomes and localizes to the rough endoplasmic reticulum JOURNAL Mol. Cell. Biol. 19 (3), 2212-2219 (1999) PUBMED 10022908 REFERENCE 10 (residues 1 to 496) AUTHORS DesGroseillers L and Lemieux N. TITLE Localization of a human double-stranded RNA-binding protein gene (STAU) to band 20q13.1 by fluorescence in situ hybridization JOURNAL Genomics 36 (3), 527-529 (1996) PUBMED 8884277 COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB100321.1, BC095397.1, BC001893.1, BC050432.1, AF061941.1, AK091405.1 and BE439382.1. On Nov 25, 2005 this sequence version replaced NP_004593.1.
Summary: Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (T4) has an additional exon in the 5' UTR compared to T2, the most abundantly expressed transcript variant. T4 encodes the same isoform (a) of 496 amino acids as T2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.3249.1, SRR1803613.62205.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## FEATURES Location/Qualifiers source 1..496 /organism="Homo sapiens" /db_xref="taxon:9606" /chromosome="20" /map="20q13.13" Protein 1..496 /product="double-stranded RNA-binding protein Staufen homolog 1 isoform a" /note="staufen, RNA binding protein, homolog 1; protein phosphatase 1, regulatory subunit 150" /calculated_mol_wt=54803 Region 105..169 /region_name="DSRM" /note="Double-stranded RNA binding motif; smart00358" /db_xref="CDD:214634" Site order(109..110,151..154,157) /site_type="other" /note="dsRNA binding site [nucleotide binding]" /db_xref="CDD:238007" Region 205..271 /region_name="DSRM" /note="Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III; cd00048" /db_xref="CDD:238007" Site order(205,211..212,254..257,260) /site_type="other" /note="dsRNA binding site [nucleotide binding]" /db_xref="CDD:238007" Region 367..476 /region_name="Staufen_C" /note="Staufen C-terminal domain; pfam16482" /db_xref="CDD:318642" CDS 1..496 /gene="STAU1" /gene_synonym="PPP1R150; STAU" /coded_by="NM_004602.3:492..1982" /note="isoform a is encoded by transcript variant T4" /db_xref="CCDS:CCDS13415.1" /db_xref="GeneID:6780" /db_xref="HGNC:HGNC:11370" /db_xref="MIM:601716" ORIGIN 1 mklgkkpmyk pvdpysrmqs tynynmrgga yppryfypfp vppllyqvel svggqqfngk 61 gktrqaakhd aaakalrilq neplperlev ngreseeenl nkseisqvfe ialkrnlpvn 121 fevaresgpp hmknfvtkvs vgefvgegeg kskkiskkna aiavleelkk lpplpaverv 181 kprikkktkp ivkpqtspey gqginpisrl aqiqqakkek epeytllter glprrrefvm 241 qvkvgnhtae gtgtnkkvak rnaaenmlei lgfkvpqaqp tkpalkseek tpikkpgdgr 301 kvtffepgsg dengtsnked efrmpylshq qlpagilpmv pevaqavgvs qghhtkdftr 361 aapnpakatv tamiarelly ggtsptaeti lknnissghv phgpltrpse qldylsrvqg 421 fqveykdfpk nnknefvsli ncssqpplis hgigkdvesc hdmaalnilk llseldqqst 481 emprtgngpm svcgrc //
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