Tälle proteiinille on tyypillsitä useampi DSRM- motiivi ( dsRNA:ta sitova motiivi) .
Vuonna 1996 on julkaistu artikkeli tätä proteiinia koodaavan geenin sijoittumisesta kromosomiin 20q13.3. Geenin nimi STAU. ( Jo Drosophilass on staufin- geeni).
Vuonna 1999 tiedetään, että Ihmesen staufen-sekvenssi on RNA:ta sitova proteiini, ja se assosioituu polysomeihin ja sijoittautuu karkeaan endoplasmiseen retikulumiin (RER). Samana vuonna havaitaan että imettäväisillä staufen liittuu tubuliiniin. Ihmisellä staufen tekee interaktion influenssaviruksen NS1-proteiinin kanssa koeputkessa ja in vivo.
Vuonna 2000 on saatu oivalluksia mRNA-kuljetuksesta ja paikallisesta translaatiosta imettäväisten hermojärjestelmässä.
On huomattu, että SNHG5 stabiloi kohdetranskriptejä blokeeraamalla niiden hajoittamisen STAU1:llä . Sen mukaan STAU1- depleetio palauttaa apoptoosin, jonka on indusoinut SNHG5 poistogeenisyys. Täten SNHG5 luonnehditaan lncRNA:ksi, joka edisiää tuumorisolun elossapysymistä kolorektaalisyövässä.
Vuonna 2016 artikkeli: SNHG5 edistää kolorektaalisyöpäsolun elossapysymistä vastavaikuttamalla STAU-1 välitteiseen mRNA:n destabilisoimiseen.
Vuonna 2017: Influenssaproteiini-interaktomeista tunnistetaan plakofiliini 2:n osuus virusrestriktiossa . Samana vuonna todetaan , että ADAR1 kontrolloi stressattujen solujen apoptoosia estämällä Staufen1- välitteisen mRNA- hajoituksen ( ADAR1p110 isoformi estää kompetitiivisesti Staufen1:n sitoutumisen dsRNA:n 3´UTR-alueeseen ja vastavaikuttaa Staufen1- välitteiseen mRNA:n hävittämiseen).
E2F1 indusoi TINCR transkriptaalisen aktiivisuuden ja kiihdyttää mahasyövän progredioitumista aktivoimalla TINCR/STAU1/CDKN2B-signalointiakselin.
lncRNA Chronos on ikääntymisen indusoima lihashypertrofian estäjä
Sitaatti STAU1 isoformi a rakenteesta:
double-stranded RNA-binding protein Staufen homolog 1 isoform a [Homo sapiens]
NCBI Reference Sequence: NP_004593.2
LOCUS NP_004593 496 aa linear PRI 24-SEP-2018
DEFINITION double-stranded RNA-binding protein Staufen homolog 1 isoform a
[Homo sapiens].
ACCESSION NP_004593
VERSION NP_004593.2
DBSOURCE REFSEQ: accession NM_004602.3
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 496)
AUTHORS Neppl RL, Wu CL and Walsh K.
TITLE lncRNA Chronos is an aging-induced inhibitor of muscle hypertrophy
JOURNAL J. Cell Biol. 216 (11), 3497-3507 (2017)
PUBMED 28855249
REFERENCE 2 (residues 1 to 496)
AUTHORS Xu TP, Wang YF, Xiong WL, Ma P, Wang WY, Chen WM, Huang MD, Xia R,
Wang R, Zhang EB, Liu YW, De W and Shu YQ.
TITLE E2F1 induces TINCR transcriptional activity and accelerates gastric
cancer progression via activation of TINCR/STAU1/CDKN2B signaling
axis
JOURNAL Cell Death Dis 8 (6), e2837 (2017)
PUBMED 28569791
REMARK GeneRIF: E2F1 induces TINCR transcriptional activity and
accelerates gastric cancer progression via activation of
TINCR/STAU1/CDKN2B signaling axis.
Publication Status: Online-Only
REFERENCE 3 (residues 1 to 496)
AUTHORS Sakurai M, Shiromoto Y, Ota H, Song C, Kossenkov AV, Wickramasinghe
J, Showe LC, Skordalakes E, Tang HY, Speicher DW and Nishikura K.
TITLE ADAR1 controls apoptosis of stressed cells by inhibiting
Staufen1-mediated mRNA decay
JOURNAL Nat. Struct. Mol. Biol. 24 (6), 534-543 (2017)
PUBMED 28436945
REMARK GeneRIF: ADAR1p110 isoform competitively inhibits binding of
Staufen1 to the 3'-untranslated-region dsRNAs and antagonizes
Staufen1-mediated mRNA decay.
REFERENCE 4 (residues 1 to 496)
AUTHORS Wang L, Fu B, Li W, Patil G, Liu L, Dorf ME and Li S.
TITLE Comparative influenza protein interactomes identify the role of
plakophilin 2 in virus restriction
JOURNAL Nat Commun 8, 13876 (2017)
PUBMED 28169297
REMARK Publication Status: Online-Only
REFERENCE 5 (residues 1 to 496)
AUTHORS Damas ND, Marcatti M, Come C, Christensen LL, Nielsen MM,
Baumgartner R, Gylling HM, Maglieri G, Rundsten CF, Seemann SE,
Rapin N, Thezenas S, Vang S, Orntoft T, Andersen CL, Pedersen JS
and Lund AH.
TITLE SNHG5 promotes colorectal cancer cell survival by counteracting
STAU1-mediated mRNA destabilization
JOURNAL Nat Commun 7, 13875 (2016)
PUBMED 28004750
REMARK GeneRIF: Mechanistically, we suggest that SNHG5 stabilizes the
target transcripts by blocking their degradation by STAU1.
Accordingly, depletion of STAU1 rescues the apoptosis induced after
SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting
tumour cell survival in colorectal cancer.
Publication Status: Online-Only
REFERENCE 6 (residues 1 to 496)
AUTHORS Kiebler MA and DesGroseillers L.
TITLE Molecular insights into mRNA transport and local translation in the
mammalian nervous system
JOURNAL Neuron 25 (1), 19-28 (2000)
PUBMED 10707969
REMARK Review article
REFERENCE 7 (residues 1 to 496)
AUTHORS Falcon AM, Fortes P, Marion RM, Beloso A and Ortin J.
TITLE Interaction of influenza virus NS1 protein and the human homologue
of Staufen in vivo and in vitro
JOURNAL Nucleic Acids Res. 27 (11), 2241-2247 (1999)
PUBMED 10325410
REFERENCE 8 (residues 1 to 496)
AUTHORS Wickham L, Duchaine T, Luo M, Nabi IR and DesGroseillers L.
TITLE Mammalian staufen is a double-stranded-RNA- and tubulin-binding
protein which localizes to the rough endoplasmic reticulum
JOURNAL Mol. Cell. Biol. 19 (3), 2220-2230 (1999)
PUBMED 10022909
REFERENCE 9 (residues 1 to 496)
AUTHORS Marion RM, Fortes P, Beloso A, Dotti C and Ortin J.
TITLE A human sequence homologue of Staufen is an RNA-binding protein
that is associated with polysomes and localizes to the rough
endoplasmic reticulum
JOURNAL Mol. Cell. Biol. 19 (3), 2212-2219 (1999)
PUBMED 10022908
REFERENCE 10 (residues 1 to 496)
AUTHORS DesGroseillers L and Lemieux N.
TITLE Localization of a human double-stranded RNA-binding protein gene
(STAU) to band 20q13.1 by fluorescence in situ hybridization
JOURNAL Genomics 36 (3), 527-529 (1996)
PUBMED 8884277
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from DB100321.1, BC095397.1,
BC001893.1, BC050432.1, AF061941.1, AK091405.1 and BE439382.1.
On Nov 25, 2005 this sequence version replaced NP_004593.1.
Summary: Staufen is a member of the family of double-stranded RNA
(dsRNA)-binding proteins involved in the transport and/or
localization of mRNAs to different subcellular compartments and/or
organelles. These proteins are characterized by the presence of
multiple dsRNA-binding domains which are required to bind RNAs
having double-stranded secondary structures. The human homologue of
staufen encoded by STAU, in addition contains a microtubule-
binding domain similar to that of microtubule-associated protein
1B, and binds tubulin. The STAU gene product has been shown to be
present in the cytoplasm in association with the rough endoplasmic
reticulum (RER), implicating this protein in the transport of mRNA
via the microtubule network to the RER, the site of translation.
Five transcript variants resulting from alternative splicing of
STAU gene and encoding three isoforms have been described. Three of
these variants encode the same isoform, however, differ in their
5'UTR. [provided by RefSeq, Jul 2008].
Transcript Variant: This variant (T4) has an additional exon in the
5' UTR compared to T2, the most abundantly expressed transcript
variant. T4 encodes the same isoform (a) of 496 amino acids as T2.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803615.3249.1,
SRR1803613.62205.1 [ECO:0000332]
RNAseq introns :: mixed/partial sample support
SAMEA1965299, SAMEA1966682
[ECO:0000350]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..496
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="20"
/map="20q13.13"
Protein 1..496
/product="double-stranded RNA-binding protein Staufen
homolog 1 isoform a"
/note="staufen, RNA binding protein, homolog 1; protein
phosphatase 1, regulatory subunit 150"
/calculated_mol_wt=54803
Region 105..169
/region_name="DSRM"
/note="Double-stranded RNA binding motif; smart00358"
/db_xref="CDD:214634"
Site order(109..110,151..154,157)
/site_type="other"
/note="dsRNA binding site [nucleotide binding]"
/db_xref="CDD:238007"
Region 205..271
/region_name="DSRM"
/note="Double-stranded RNA binding motif. Binding is not
sequence specific but is highly specific for double
stranded RNA. Found in a variety of proteins including
dsRNA dependent protein kinase PKR, RNA helicases,
Drosophila staufen protein, E. coli RNase III; cd00048"
/db_xref="CDD:238007"
Site order(205,211..212,254..257,260)
/site_type="other"
/note="dsRNA binding site [nucleotide binding]"
/db_xref="CDD:238007"
Region 367..476
/region_name="Staufen_C"
/note="Staufen C-terminal domain; pfam16482"
/db_xref="CDD:318642"
CDS 1..496
/gene="STAU1"
/gene_synonym="PPP1R150; STAU"
/coded_by="NM_004602.3:492..1982"
/note="isoform a is encoded by transcript variant T4"
/db_xref="CCDS:CCDS13415.1"
/db_xref="GeneID:6780"
/db_xref="HGNC:HGNC:11370"
/db_xref="MIM:601716"
ORIGIN
1 mklgkkpmyk pvdpysrmqs tynynmrgga yppryfypfp vppllyqvel svggqqfngk
61 gktrqaakhd aaakalrilq neplperlev ngreseeenl nkseisqvfe ialkrnlpvn
121 fevaresgpp hmknfvtkvs vgefvgegeg kskkiskkna aiavleelkk lpplpaverv
181 kprikkktkp ivkpqtspey gqginpisrl aqiqqakkek epeytllter glprrrefvm
241 qvkvgnhtae gtgtnkkvak rnaaenmlei lgfkvpqaqp tkpalkseek tpikkpgdgr
301 kvtffepgsg dengtsnked efrmpylshq qlpagilpmv pevaqavgvs qghhtkdftr
361 aapnpakatv tamiarelly ggtsptaeti lknnissghv phgpltrpse qldylsrvqg
421 fqveykdfpk nnknefvsli ncssqpplis hgigkdvesc hdmaalnilk llseldqqst
481 emprtgngpm svcgrc
//
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