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tisdag 16 oktober 2018

dsRNA:ta sitova ihmisen soluproteiini Staufen

Otan  yhden staufen-proteiinin peptidirakenteen tähän esimerkiksi. isoformi a.
 Tälle proteiinille on tyypillsitä useampi DSRM- motiivi ( dsRNA:ta sitova  motiivi) .
Vuonna 1996  on  julkaistu artikkeli tätä  proteiinia koodaavan geenin  sijoittumisesta kromosomiin  20q13.3.   Geenin nimi STAU.  ( Jo Drosophilass on staufin- geeni).
Vuonna 1999 tiedetään, että Ihmesen  staufen-sekvenssi on RNA:ta sitova proteiini, ja se assosioituu polysomeihin ja sijoittautuu karkeaan endoplasmiseen retikulumiin  (RER). Samana vuonna havaitaan että imettäväisillä  staufen liittuu tubuliiniin. Ihmisellä staufen tekee interaktion influenssaviruksen NS1-proteiinin kanssa  koeputkessa ja in vivo.
Vuonna 2000 on saatu oivalluksia mRNA-kuljetuksesta ja paikallisesta translaatiosta imettäväisten hermojärjestelmässä.
On huomattu, että SNHG5 stabiloi kohdetranskriptejä blokeeraamalla niiden  hajoittamisen  STAU1:llä . Sen mukaan  STAU1- depleetio palauttaa apoptoosin, jonka on indusoinut SNHG5  poistogeenisyys.  Täten SNHG5  luonnehditaan  lncRNA:ksi, joka edisiää tuumorisolun elossapysymistä kolorektaalisyövässä.
Vuonna 2016 artikkeli: SNHG5 edistää kolorektaalisyöpäsolun elossapysymistä vastavaikuttamalla STAU-1 välitteiseen  mRNA:n  destabilisoimiseen.
Vuonna 2017: Influenssaproteiini-interaktomeista  tunnistetaan plakofiliini 2:n osuus   virusrestriktiossa . Samana vuonna  todetaan , että ADAR1 kontrolloi  stressattujen solujen apoptoosia estämällä Staufen1- välitteisen mRNA- hajoituksen ( ADAR1p110 isoformi estää kompetitiivisesti Staufen1:n  sitoutumisen  dsRNA:n  3´UTR-alueeseen  ja vastavaikuttaa Staufen1- välitteiseen mRNA:n hävittämiseen).
E2F1 indusoi TINCR transkriptaalisen aktiivisuuden ja kiihdyttää mahasyövän progredioitumista aktivoimalla TINCR/STAU1/CDKN2B-signalointiakselin.
lncRNA Chronos on ikääntymisen indusoima lihashypertrofian estäjä

  • Sitaatti  STAU1 isoformi a rakenteesta: 

double-stranded RNA-binding protein Staufen homolog 1 isoform a [Homo sapiens]

NCBI Reference Sequence: NP_004593.2

LOCUS       NP_004593                496 aa            linear   PRI 24-SEP-2018
DEFINITION  double-stranded RNA-binding protein Staufen homolog 1 isoform a
            [Homo sapiens].
ACCESSION   NP_004593
VERSION     NP_004593.2
DBSOURCE    REFSEQ: accession NM_004602.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 496)
  AUTHORS   Neppl RL, Wu CL and Walsh K.
  TITLE     lncRNA Chronos is an aging-induced inhibitor of muscle hypertrophy
  JOURNAL   J. Cell Biol. 216 (11), 3497-3507 (2017)
   PUBMED   28855249
REFERENCE   2  (residues 1 to 496)
  AUTHORS   Xu TP, Wang YF, Xiong WL, Ma P, Wang WY, Chen WM, Huang MD, Xia R,
            Wang R, Zhang EB, Liu YW, De W and Shu YQ.
  TITLE     E2F1 induces TINCR transcriptional activity and accelerates gastric
            cancer progression via activation of TINCR/STAU1/CDKN2B signaling
            axis
  JOURNAL   Cell Death Dis 8 (6), e2837 (2017)
   PUBMED   28569791
  REMARK    GeneRIF: E2F1 induces TINCR transcriptional activity and
            accelerates gastric cancer progression via activation of
            TINCR/STAU1/CDKN2B signaling axis.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 496)
  AUTHORS   Sakurai M, Shiromoto Y, Ota H, Song C, Kossenkov AV, Wickramasinghe
            J, Showe LC, Skordalakes E, Tang HY, Speicher DW and Nishikura K.
  TITLE     ADAR1 controls apoptosis of stressed cells by inhibiting
            Staufen1-mediated mRNA decay
  JOURNAL   Nat. Struct. Mol. Biol. 24 (6), 534-543 (2017)
   PUBMED   28436945
  REMARK    GeneRIF: ADAR1p110 isoform competitively inhibits binding of
            Staufen1 to the 3'-untranslated-region dsRNAs and antagonizes
            Staufen1-mediated mRNA decay.
REFERENCE   4  (residues 1 to 496)
  AUTHORS   Wang L, Fu B, Li W, Patil G, Liu L, Dorf ME and Li S.
  TITLE     Comparative influenza protein interactomes identify the role of
            plakophilin 2 in virus restriction
  JOURNAL   Nat Commun 8, 13876 (2017)
   PUBMED   28169297
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 496)
  AUTHORS   Damas ND, Marcatti M, Come C, Christensen LL, Nielsen MM,
            Baumgartner R, Gylling HM, Maglieri G, Rundsten CF, Seemann SE,
            Rapin N, Thezenas S, Vang S, Orntoft T, Andersen CL, Pedersen JS
            and Lund AH.
  TITLE     SNHG5 promotes colorectal cancer cell survival by counteracting
            STAU1-mediated mRNA destabilization
  JOURNAL   Nat Commun 7, 13875 (2016)
   PUBMED   28004750
  REMARK    GeneRIF: Mechanistically, we suggest that SNHG5 stabilizes the
            target transcripts by blocking their degradation by STAU1.
            Accordingly, depletion of STAU1 rescues the apoptosis induced after
            SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting
            tumour cell survival in colorectal cancer.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 496)
  AUTHORS   Kiebler MA and DesGroseillers L.
  TITLE     Molecular insights into mRNA transport and local translation in the
            mammalian nervous system
  JOURNAL   Neuron 25 (1), 19-28 (2000)
   PUBMED   10707969
  REMARK    Review article
REFERENCE   7  (residues 1 to 496)
  AUTHORS   Falcon AM, Fortes P, Marion RM, Beloso A and Ortin J.
  TITLE     Interaction of influenza virus NS1 protein and the human homologue
            of Staufen in vivo and in vitro
  JOURNAL   Nucleic Acids Res. 27 (11), 2241-2247 (1999)
   PUBMED   10325410
REFERENCE   8  (residues 1 to 496)
  AUTHORS   Wickham L, Duchaine T, Luo M, Nabi IR and DesGroseillers L.
  TITLE     Mammalian staufen is a double-stranded-RNA- and tubulin-binding
            protein which localizes to the rough endoplasmic reticulum
  JOURNAL   Mol. Cell. Biol. 19 (3), 2220-2230 (1999)
   PUBMED   10022909
REFERENCE   9  (residues 1 to 496)
  AUTHORS   Marion RM, Fortes P, Beloso A, Dotti C and Ortin J.
  TITLE     A human sequence homologue of Staufen is an RNA-binding protein
            that is associated with polysomes and localizes to the rough
            endoplasmic reticulum
  JOURNAL   Mol. Cell. Biol. 19 (3), 2212-2219 (1999)
   PUBMED   10022908
REFERENCE   10 (residues 1 to 496)
  AUTHORS   DesGroseillers L and Lemieux N.
  TITLE     Localization of a human double-stranded RNA-binding protein gene
            (STAU) to band 20q13.1 by fluorescence in situ hybridization
  JOURNAL   Genomics 36 (3), 527-529 (1996)
   PUBMED   8884277
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB100321.1, BC095397.1,
            BC001893.1, BC050432.1, AF061941.1, AK091405.1 and BE439382.1.
            On Nov 25, 2005 this sequence version replaced NP_004593.1
  Summary: Staufen is a member of the family of double-stranded RNA
            (dsRNA)-binding proteins involved in the transport and/or
            localization of mRNAs to different subcellular compartments and/or
            organelles. These proteins are characterized by the presence of
            multiple dsRNA-binding domains which are required to bind RNAs
            having double-stranded secondary structures. The human homologue of
            staufen encoded by STAU, in addition contains a microtubule-
            binding domain similar to that of microtubule-associated protein
            1B, and binds tubulin. The STAU gene product has been shown to be
            present in the cytoplasm in association with the rough endoplasmic
            reticulum (RER), implicating this protein in the transport of mRNA
            via the microtubule network to the RER, the site of translation.
            Five transcript variants resulting from alternative splicing of
            STAU gene and encoding three isoforms have been described. Three of
            these variants encode the same isoform, however, differ in their
            5'UTR. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (T4) has an additional exon in the
            5' UTR compared to T2, the most abundantly expressed transcript
            variant. T4 encodes the same isoform (a) of 496 amino acids as T2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.3249.1,
                                           SRR1803613.62205.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     Protein         1..496
                     /product="double-stranded RNA-binding protein Staufen
                     homolog 1 isoform a"
                     /note="staufen, RNA binding protein, homolog 1; protein
                     phosphatase 1, regulatory subunit 150"
                     /calculated_mol_wt=54803
     Region          105..169
                     /region_name="DSRM"
                     /note="Double-stranded RNA binding motif; smart00358"
                     /db_xref="CDD:214634"
     Site            order(109..110,151..154,157)
                     /site_type="other"
                     /note="dsRNA binding site [nucleotide binding]"
                     /db_xref="CDD:238007"
     Region          205..271
                     /region_name="DSRM"
                     /note="Double-stranded RNA binding motif. Binding is not
                     sequence specific but is highly specific for double
                     stranded RNA. Found in a variety of proteins including
                     dsRNA dependent protein kinase PKR, RNA helicases,
                     Drosophila staufen protein, E. coli RNase III; cd00048"
                     /db_xref="CDD:238007"
     Site            order(205,211..212,254..257,260)
                     /site_type="other"
                     /note="dsRNA binding site [nucleotide binding]"
                     /db_xref="CDD:238007"
     Region          367..476
                     /region_name="Staufen_C"
                     /note="Staufen C-terminal domain; pfam16482"
                     /db_xref="CDD:318642"
     CDS             1..496
                     /gene="STAU1"
                     /gene_synonym="PPP1R150; STAU"
                     /coded_by="NM_004602.3:492..1982"
                     /note="isoform a is encoded by transcript variant T4"
                     /db_xref="CCDS:CCDS13415.1"
                     /db_xref="GeneID:6780"
                     /db_xref="HGNC:HGNC:11370"
                     /db_xref="MIM:601716"
ORIGIN      
        1 mklgkkpmyk pvdpysrmqs tynynmrgga yppryfypfp vppllyqvel svggqqfngk
       61 gktrqaakhd aaakalrilq neplperlev ngreseeenl nkseisqvfe ialkrnlpvn
      121 fevaresgpp hmknfvtkvs vgefvgegeg kskkiskkna aiavleelkk lpplpaverv
      181 kprikkktkp ivkpqtspey gqginpisrl aqiqqakkek epeytllter glprrrefvm
      241 qvkvgnhtae gtgtnkkvak rnaaenmlei lgfkvpqaqp tkpalkseek tpikkpgdgr
      301 kvtffepgsg dengtsnked efrmpylshq qlpagilpmv pevaqavgvs qghhtkdftr
      361 aapnpakatv tamiarelly ggtsptaeti lknnissghv phgpltrpse qldylsrvqg
      421 fqveykdfpk nnknefvsli ncssqpplis hgigkdvesc hdmaalnilk llseldqqst
      481 emprtgngpm svcgrc
//

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