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tisdag 5 september 2017

Koetan katsoa miten borrelian koneisto käyttää isäntäsolua.

 Modulation of Host immunity
https://www.researchgate.net/publication/280639883_Modulation_of_host_immunity_by_tick_saliva

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Items: 1 to 20 of 37

1.
Adams PP, Flores Avile C, Popitsch N, Bilusic I, Schroeder R, Lybecker M, Jewett MW.
Nucleic Acids Res. 2017 Jan 25;45(2):775-792. doi: 10.1093/nar/gkw1180. Epub 2016 Dec 1.
2.
Smith AA, Navasa N, Yang X, Wilder CN, Buyuktanir O, Marques A, Anguita J, Pal U.
Cell Host Microbe. 2016 Jul 13;20(1):91-8. doi: 10.1016/j.chom.2016.06.001. Epub 2016 Jun 30.
3.
Bouquet J, Soloski MJ, Swei A, Cheadle C, Federman S, Billaud JN, Rebman AW, Kabre B, Halpert R, Boorgula M, Aucott JN, Chiu CY.
MBio. 2016 Feb 12;7(1):e00100-16. doi: 10.1128/mBio.00100-16.

Abstract

Lyme disease is a tick-borne illness caused by the bacterium Borrelia burgdorferi, and approximately 10 to 20% of patients report persistent symptoms lasting months to years despite appropriate treatment with antibiotics. To gain insights into the molecular basis of acute Lyme disease and the ensuing development of post-treatment symptoms, we conducted a longitudinal transcriptome study of 29 Lyme disease patients (and 13 matched controls) enrolled at the time of diagnosis and followed for up to 6 months. The differential gene expression signature of Lyme disease following the acute phase of infection persisted for at least 3 weeks and had fewer than 44% differentially expressed genes (DEGs) in common with other infectious or noninfectious syndromes. Early Lyme disease prior to antibiotic therapy was characterized by marked upregulation of Toll-like receptor signaling but lack of activation of the inflammatory T-cell apoptotic and B-cell developmental pathways seen in other acute infectious syndromes. Six months after completion of therapy, Lyme disease patients were found to have 31 to 60% of their pathways in common with three different immune-mediated chronic diseases. No differential gene expression signature was observed between Lyme disease patients with resolved illness to those with persistent symptoms at 6 months post-treatment. The identification of a sustained differential gene expression signature in Lyme disease suggests that a panel of selected human host-based biomarkers may address the need for sensitive clinical diagnostics during the "window period" of infection prior to the appearance of a detectable antibody response and may also inform the development of new therapeutic targets.

IMPORTANCE:

Lyme disease is the most common tick-borne infection in the United States, and some patients report lingering symptoms lasting months to years despite antibiotic treatment. To better understand the role of the human host response in acute Lyme disease and the development of post-treatment symptoms, we conducted the first longitudinal gene expression (transcriptome) study of patients enrolled at the time of diagnosis and followed up for up to 6 months after treatment. Importantly, we found that the gene expression signature of early Lyme disease is distinct from that of other acute infectious diseases and persists for at least 3 weeks following infection. This study also uncovered multiple previously undescribed pathways and genes that may be useful in the future as human host biomarkers for diagnosis and that constitute potential targets for the development of new therapies.
Free PMC Article
4.
Fazzino L, Tilly K, Dulebohn DP, Rosa PA.
Infect Immun. 2015 Dec;83(12):4800-10. doi: 10.1128/IAI.00925-15. Epub 2015 Oct 5.
5.
Drecktrah D, Lybecker M, Popitsch N, Rescheneder P, Hall LS, Samuels DS.
PLoS Pathog. 2015 Sep 15;11(9):e1005160. doi: 10.1371/journal.ppat.1005160. eCollection 2015 Sep. Erratum in: PLoS Pathog. 2015 Oct;11(10):e1005242.
6.
Martin CL, Sukarna TY, Akther S, Ramrattan G, Pagan P, Di L, Mongodin EF, Fraser CM, Schutzer SE, Luft BJ, Casjens SR, Qiu WG.
MBio. 2015 Apr 14;6(2). pii: e00011-15. doi: 10.1128/mBio.00011-15. Erratum in: MBio. 2015;6(4):e00999. Martin, Che I [Corrected to Martin, Che L].
7.
Iyer R, Caimano MJ, Luthra A, Axline D Jr, Corona A, Iacobas DA, Radolf JD, Schwartz I.
Mol Microbiol. 2015 Feb;95(3):509-38. doi: 10.1111/mmi.12882. Epub 2014 Dec 30.
8.
Lieskovská J, Páleníková J, Širmarová J, Elsterová J, Kotsyfakis M, Campos Chagas A, Calvo E, Růžek D, Kopecký J.
Parasite Immunol. 2015 Feb;37(2):70-8. doi: 10.1111/pim.12162.
9.
Narasimhan S, Rajeevan N, Liu L, Zhao YO, Heisig J, Pan J, Eppler-Epstein R, Deponte K, Fish D, Fikrig E.
Cell Host Microbe. 2014 Jan 15;15(1):58-71. doi: 10.1016/j.chom.2013.12.001.
10.
Wang P, Dadhwal P, Cheng Z, Zianni MR, Rikihisa Y, Liang FT, Li X.
Mol Microbiol. 2013 Sep;89(6):1140-53. doi: 10.1111/mmi.12337. Epub 2013 Aug 14.
11.
Schramm F, Kern A, Barthel C, Nadaud S, Meyer N, Jaulhac B, Boulanger N.
PLoS One. 2012;7(6):e40046. doi: 10.1371/journal.pone.0040046. Epub 2012 Jun 29.
12.
Lieskovska J, Kopecky J.
Parasite Immunol. 2012 Aug-Sep;34(8-9):421-9. doi: 10.1111/j.1365-3024.2012.01375.x.
13.
Lieskovská J, Kopecký J.
Parasite Immunol. 2012 Jan;34(1):32-9. doi: 10.1111/j.1365-3024.2011.01345.x.

Abstract

Dendritic cells are a sentinel in defending against pathogens and tick saliva facilitates transmission of tick-borne pathogens by modulating the host immune response. The maturation of dendritic cells is inhibited by tick saliva. To elucidate the mechanism of this inhibition, we tested the impact of Ixodes ricinus tick saliva on signalling pathways activated by Toll-like receptor (TLR-2) ligand and Borrelia afzelii in spleen dendritic cells. The activation of nuclear factor-κB (NF-κB) p65 and phosphatidylinositol-3 kinase (PI3K)/Akt pathways was decreased by tick saliva upon both TLR-2 and Borrelia stimulation. Among the mitogen-activated protein kinases (MAPK), the activation of extracellular matrix-regulated kinase (Erk1/2) was suppressed by tick saliva, but not p38. In response to spirochaetes, the amount of TNF-α decreased in the presence of tick saliva which was mediated by selective suppression of Erk1/2, NF-κB and Akt as tick saliva mimicked the effect of their specific inhibitors, UO126, IKK-IV and LY294002, respectively. Saliva-induced enhancement of IL-10 was not observed in the presence of specific inhibitor of Protein Kinase A (PKA), H-89, suggesting the involvement of PKA pathway in IL-10 production. Our cumulative data show that tick saliva interferes with several signalling pathways, thus modulating the immune functions of dendritic cells.
PMID:
22709526
DOI:
10.1111/j.1365-3024.2012.01375.x
[Indexed for MEDLINE]
Free full text
Free Article
14.
Sahay B, Singh A, Gnanamani A, Patsey RL, Blalock JE, Sellati TJ.
Am J Pathol. 2011 Feb;178(2):724-34. doi: 10.1016/j.ajpath.2010.10.025.
15.
Zhang X, Yang X, Kumar M, Pal U.
J Infect Dis. 2009 Oct 15;200(8):1318-30. doi: 10.1086/605846.
16.
17.
Srivastava SY, de Silva AM.
J Bacteriol. 2008 May;190(10):3429-33. doi: 10.1128/JB.00085-08. Epub 2008 Mar 21.
18.
Cruz AR, Moore MW, La Vake CJ, Eggers CH, Salazar JC, Radolf JD.
Infect Immun. 2008 Jan;76(1):56-70. Epub 2007 Oct 15.
20.
Behera AK, Hildebrand E, Szafranski J, Hung HH, Grodzinsky AJ, Lafyatis R, Koch AE, Kalish R, Perides G, Steere AC, Hu LT.
Arthritis Rheum. 2006 Oct;54(10):3319-29.

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