tetraspaniini-7 proteiini PubMed hakulaiteella

COMMENT     Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="X"
     Protein         1..249
                     /product="tetraspanin 7, isoform CRA_a"
     Region          14..241
                     /region_name="Tetraspannin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:249779"
     Region          110..213
                     /region_name="TM4SF2_6_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF2_6_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03161"
                     /db_xref="CDD:239414"
     Site            order(111,116,120,122..123,126,140,143..144,147..148)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239414"
     CDS             1..249
                     /gene="TSPAN7"
                     /locus_tag="hCG_18324"
                     /coded_by="join(CH471141.2:1274072..1274152,
                     CH471141.2:1378638..1378826,CH471141.2:1383893..1383967,
                     CH471141.2:1386738..1386833,CH471141.2:1388222..1388377,
                     CH471141.2:1393722..1393805,CH471141.2:1400117..1400185)"
                     /note="gene_id=hCG18324.3 transcript_id=hCT1969285.1
                     protein_id=hCP1782967.1 isoform=CRA_a"
                     /db_xref="GeneID:7102"
ORIGIN      
        1 masrrmetkp vitclktlli iysfvfwitg villavgvwg kltlgtyisl iaenstnapy
       61 vligtgttiv vfglfgcfat crgspwmlkl yamflslvfl aelvagisgf vfrheikdtf
      121 lrtytdamqt yngndersra vdhvqrslsc cgvqnytnws tspyflehgi ppsccmnetd
      181 cnpqdlhnlt vaatkvnqkg cydlvtsfme tnmgiiagva fgiafsqlig mllacclsrf
      241 itanqyemv
// Essentiellit aminohapot muodostavat proteiinin alkupäässä muutamia pitkiä 

jaksoja  jopa 7 ja 6 essentielliä aminohappoa peräkkäin

Diabetes. 2016 Mar 7. pii: db151058. [Epub ahead of print]

Identification of Tetraspanin-7 as a Target of Autoantibodies in Type 1 Diabetes.

McLaughlin KA¹, Richardson CC², Ravishankar A¹, Brigatti C³, Liberati D⁴, Lampasona V⁴, Piemonti L³, Morgan D⁵, Feltbower RG⁵, Christie MR⁶.

Author information

Abstract

The presence of autoantibodies to multiple islet autoantigens confers high risk for development of Type 1 diabetes. Four major autoantigens are established (insulin, glutamate decarboxylase, IA-2, and zinc transporter-8), but the molecular identity of a fifth, a 38kDa membrane glycoprotein (Glima), is unknown. Glima antibodies have been detectable only by immunoprecipitation from extracts of radiolabeled islet or neuronal cells. We sought to identify Glima to enable efficient assay of these autoantibodies. Mouse brain and lung were shown to express Glima. Membrane glycoproteins from extracts of these organs were enriched by detergent phase separation, lectin affinity chromatography and SDS-PAGE. Proteins were also immunoaffinity purified from brain extracts using autoantibodies from diabetic patients' sera before SDS-PAGE. Eluates from gel regions equivalent to 38kDa were analyzed by LC-MS/MS for protein identification. Three proteins were detected in samples from the brain and lung extracts, and in the immunoaffinity purified sample, but not the negative control. Only tetraspanin-7, a multipass transmembrane glycoprotein with neuroendocrine expression, had physical characteristics expected of Glima. Tetraspanin-7 was confirmed as an autoantigen by demonstrating binding to autoantibodies in Type 1 diabetes. We identify tetraspanin-7 as a target of autoimmunity in diabetes, allowing its exploitation for diabetes prediction and immunotherapy.
© 2016 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.