KCTD5(16p13.3) . ( Stabiloi RNF711 sinkkisormiproteiinia).

https://www.ncbi.nlm.nih.gov/gene/54442

1. NM_018992.4 → NP_061865.1  BTB/POZ domain-containing protein KCTD5
   See identical proteins and their annotated locations for NP_061865.1

   Conserved Domains (2) summary

   smart00225
   Location:45 → 145

   BTB; Broad-Complex, Tramtrack and Bric a brac
   
   

   

   
   

   Broad-Complex, Tramtrack and Bric a brac

   Domain in Broad-Complex, Tramtrack and Bric a brac. Also known as POZ (poxvirus and zinc finger) domain. Known to be a protein-protein interaction motif found at the N-termini of several C2H2-type transcription factors as well as Shaw-type potassium channels. Known structure reveals a tightly intertwined dimer formed via interactions between N-terminal strand and helix structures. However in a subset of BTB/POZ domains, these two secondary structures appear to be missing. Be aware SMART predicts BTB/POZ domains without the beta1- and alpha1-secondary structures.

   pfam02214
   Location:46 → 135

   BTB_2; BTB/POZ domain

Related articles in PubMed

1. Genetic wiring maps of single-cell protein states reveal an off-switch for GPCR signalling. Brockmann M, et al. Nature, 2017 Jun 8. PMID 28562590
2. Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2). Weger S, et al. Virology, 2007 May 25. PMID 17239418
3. Cullin3/KCTD5 induces monoubiquitination of ΔNp63α and impairs its activity. He H, et al. FEBS Lett, 2018 Jul. PMID 29782646
4. Molecular recognition of Cullin3 by KCTDs: insights from experimental and computational investigations. Balasco N, et al. Biochim Biophys Acta, 2014 Jul. PMID 24747150
5. KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases. Bayón Y, et al. FEBS J, 2008 Aug. PMID 18573101. Potassium channel tetramerization domain (KCTD) proteins contain a bric-a-brac, tramtrak and broad complex (BTB) domain that is most similar to the tetramerization domain (T1) of voltage-gated potassium channels. Some BTB-domain-containing proteins have been shown recently to participate as substrate-specific adaptors in multimeric cullin E3 ligase reactions by recruiting proteins for ubiquitination and subsequent degradation by the proteasome. Twenty-two KCTD proteins have been found in the human genome, but their functions are largely unknown. In this study, we have characterized KCTD5, a new KCTD protein found in the cytosol of cultured cell lines. The expression of KCTD5 was upregulated post-transcriptionally in peripheral blood lymphocytes stimulated through the T-cell receptor. KCTD5 interacted specifically with cullin3, bound ubiquitinated proteins, and formed oligomers through its BTB domain. Analysis of the interaction with cullin3 showed that, in addition to the BTB domain, some amino acids in the N-terminus of KCTD5 are required for binding to cullin3. These findings suggest that KCTD5 is a substrate-specific adaptor for cullin3-based E3 ligases.

See all (43) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

What's a GeneRIF?

1. Cullin3/KCTD5 downregulates the DNA-binding affinity of DeltaNp63alpha, impairing either its transactivity or its transinhibitory activity. Functionally, Cullin3/KCTD5 abates the proproliferation activity of DeltaNp63alpha. These findings suggest that KCTD5-based CRL3 may mediate monoubiquitination and is a novel regulator of DeltaNp63alpha.
2. genetic modifier screen exposes suppressors of the KCTD5 phenotype and mechanistically demonstrates that KCTD5 acts as an off-switch for GPCR signalling by triggering proteolysis of Gbetagamma heterodimers dissociated from the Galpha subunit
3. Identification of MCM7, ZNF711 and FAM193 as KCTD5 interaction partners.KCTD5 is not involved in polyubiquitylation of MCM7 replication factor.The KCTD5/cullin3 complex stabilizes ZNF711 transcription factor.
4. structure reveals assemblies of five subunits while tetramers were anticipated; pentameric stoichiometry is observed also in solution by scanning transmission electron microscopy mass analysis and analytical ultracentrifugation
5. Analysis of the interaction with cullin3 showed that, in addition to the BTB domain, some amino acids in the N-terminus of KCTD5 are required for binding to cullin3. Findings suggest that KCTD5 is a substrate-specific adaptor for cullin3-based E3 ligases
6. we have identified a novel cellular AAV-2 Rep78/Rep68 interaction partner, KCTD5, located predominantly in the cytoplasm.

Submit: New GeneRIF Correction
HUOMAA X kromosomissa sijaitsevan RNF711 merkitys! Se stabilisoituu KCTD5/CUL3 kompkleksin  avulla.   Enemmäntietoa RNF711 sinkkisormi  RING proteiinista. jonka varsinainen oma funktio on tuntematon.
https://www.ncbi.nlm.nih.gov/gene/7552