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tisdag 7 juli 2020

LARP1, , EIF4E2, PABC1 SARS2- viruksen interaktiopåroteiineja. LARP4B ja LARP7

 Olen lukemassa tänään ribosomista. Johtoartikkelinani on lähde:https://www.aging-us.com/article/101922/text

Katson lisätietoa näistä proteiineista,  koska  ne kuuluvat 332 SARS2 viruksen  proteiini-proteiini interaktioproteiinein (ppi)  joukkoon.

LARP1, interaktioproteiini SARS2  N. 
LARP7, interaktiopoteiini SARS2 nsp8.
LARP4B interaktioåproteiini SARS2  nsp12 
LARP tarkoittaa  La Ribonucleoprotein domain  family member  ( 1, 2 , ...  etc)

  • LARP1, (5q33.2) , La related protein 1
https://www.genecards.org/cgi-bin/carddisp.pl?gene=LARP1&keywords=LARP1
Aliases for LARP1 Gene
La Ribonucleoprotein 1, Translational Regulator 2 3
La Ribonucleoprotein Domain Family Member 1 2 3 4 5
La-Related Protein 1 3 4
LARP 3 4
La Ribonucleoprotein Domain Family, Member 1 2
KIAA0731 4
GeneCards Summary for LARP1 Gene
LARP1 (La Ribonucleoprotein 1, Translational Regulator) is a Protein Coding gene. Diseases associated with LARP1 include Hepatocellular Carcinoma. Among its related pathways are Translational Control. Gene Ontology (GO) annotations related to this gene include translation initiation factor binding. An important paralog of this gene is LARP1B.
UniProtKB/Swiss-Prot Summary for LARP1 Gene
RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122).
 Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:26206669, PubMed:28379136). 
The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:28379136). 
 When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). 
Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714).
 Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543 LARP1_HUMAN,Q6PKG0 (Microbial infection) Positively regulates the replication of dengue virus (DENV). LARP1_HUMAN,Q6PKG0.
Protein attributes for LARP1 Gene
Size: 1096 amino acids. Molecular mass: 123510 Da Quaternary structure:Interacts with PABPC1/PABP (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:23711370, PubMed:28650797). Interacts with EIF4A1 (PubMed:24532714). Interacts with RPTOR (PubMed:24532714, PubMed:25940091, PubMed:28650797). Recruited to the active mTORC1 complex via interaction with RPTOR (PubMed:25940091, PubMed:28650797). Inhibition of mTORC1 activity strongly reduces interaction with RPTOR and the mTORC1 complex (PubMed:25940091, PubMed:28650797). Identified in a complex with mRNA, PABPC1**, EIF4E* and EIF4G1 (PubMed:20430826, PubMed:28650797). Found in a complex with PABPC1 and SHFL (PubMed:26735137). 
Tässä kommentti:  Mainittu EIF4E*(2q37.1) , (eukaryotic translation initiation factor 4E family member 2)  on SARS2 viruksen nsp2 proteiinin interaktioproteiini.
Mainittu PABPC1 **(8q22.3) , (poly A binding protein, cytoplasmic 1, RNA binding motif containing ) tekee interaktion SARS2 N-proteiinin kanssa.

  • LARP4B, (10p15.3), La-Related Protein 4B
Aliases for LARP4B Gene
La Ribonucleoprotein 4B 2 3
La Ribonucleoprotein Domain Family Member 4B 2 3 4 5
KIAA0217 2 3 4
La Ribonucleoprotein Domain Family, Member 5 2 3
La-Related Protein 4B 3 4
La-Related Protein 5 3 4
LARP5 3 4
La Ribonucleoprotein Domain Family, Member 4B 2
La Ribonucleoprotein Domain Family Member 5 4
 LARP4B Gene. This gene encodes a member of an evolutionarily conserved protein family implicated in RNA metabolism and translation. Members of this family are characterized by the presence of an La motif, which is often located adjacent to one or more RNA recognition motifs (RRM). Together, the two motifs constitute the functional region of the protein and enable its interaction with the RNA substrate. This protein family is divided into five sub-families: the genuine La proteins and four La-related protein (LARP) sub-families. The protein encoded by this gene belongs to LARP sub-family 4. It is a cytoplasmic protein that may play a stimulatory role in translation. [provided by RefSeq, Oct 2012] GeneCards Summary for LARP4B Gene
LARP4B (La Ribonucleoprotein 4B) is a Protein Coding gene. An important paralog of this gene is LARP4.  UniProtKB/Swiss-Prot Summary for LARP4B Gene Stimulates mRNA translation. LAR4B_HUMAN,Q92615
Protein attributes for LARP4B Gene Size: 738 amino acids Molecular mass: 80552 Da
Quaternary structure: Interacts with PABPC1 *(PubMed:20573744, Ref.23). Interacts with RACK1 (PubMed:20573744). Associates with polysomes via the 40S ribosomal subunit (PubMed:20573744).
  • LARP7, (4q25), La-related protein 7
https://www.genecards.org/cgi-bin/carddisp.pl?gene=LARP7&keywords=LARP7 
Aliases for LARP7 Gene
La Ribonucleoprotein 7, Transcriptional Regulator 2 3
La Ribonucleoprotein Domain Family Member 7 2 3 4 5
P-TEFb-Interaction Protein For 7SK Stability 2 3 4
La-Related Protein 7 3 4
PIP7S 3 4
La Ribonucleoprotein Domain Family, Member 7 2
HDCMA18P 3
ALAZS 3
 This gene encodes a protein which is found in the 7SK snRNP (small nuclear ribonucleoprotein). This snRNP complex inhibits a cyclin-dependent kinase, positive transcription elongation factor b, which is required for paused RNA polymerase II at a promoter to begin transcription elongation. A pseudogene of this gene is located on chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
   GeneCards Summary for LARP7 gene  (La Ribonucleoprotein 7, Transcriptional Regulator) is a Protein Coding gene. Diseases associated with LARP7 include Alazami Syndrome and Autosomal Dominant Non-Syndromic Intellectual Disability 27. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding. An important paralog of this gene is SSB.
UniProtKB/Swiss-Prot Summary for LARP7 Gene: Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. LARP7_HUMAN,Q4G0J3

 https://en.wikipedia.org/wiki/7SK_RNA

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