Proteosomiaktivaattori PA28 (2017)

http://www.cell.com/structure/fulltext/S0969-2126%2817%2930247-2

Highlights

• •Crystal structures of mouse PA28α₇, PA28β₇, and PA28α₄β₃ were determined
• •Despite forming a heptamer PA28β fails to bind to proteasomes
• •The alternating arrangement of four α and three β subunits in PA28αβ is unchangeable
• •β-β and α-α contacts are less stable than α-β interfaces

Summary

The heptameric proteasome activator (PA) 28αβ is known to modulate class I antigen processing by docking onto 20S proteasome core particles (CPs). The exact stoichiometry and arrangement of its α and β subunits, however, is still controversial. Here we analyzed murine PA28 complexes regarding structure and assembly. Strikingly, PA28α, PA28β, and PA28αβ preparations form heptamers, but solely PA28α and PA28αβ associate with CPs. Co-expression of α and β yields one unique PA28αβ species with an unchangeable subunit composition. Structural data on PA28α, PA28β, and PA28αβ up to 2.9 Å resolution reveal a PA28α₄β₃ complex with an alternating subunit arrangement and a single α-α interface. Differential scanning fluorimetry experiments and activity assays classify PA28α₄β₃ as most stable and most active, indicating that this assembly might represent the physiologically relevant species. Together, our data resolve subunit composition and arrangement of PA28αβ and clarify how an asymmetric heptamer can be assembled from two highly homologous subunits.