https://www.ncbi.nlm.nih.gov/pubmed/31514316
Abstract
Diets high in
saturated fatty acids (FA) represent a risk factor for the development
of obesity and associated metabolic disorders, partly through their
impact on the epithelial cell barrier integrity. We hypothesized that
unsaturated FA could alleviate saturated FA-induced endoplasmic
reticulum (ER) stress occurring in intestinal secretory goblet cells,
and consequently the reduced synthesis and secretion of mucins
that form the protective mucus barrier. To investigate this hypothesis,
we treated well-differentiated human colonic LS174T goblet cells with
palmitic acid (PAL)-the most commonly used inducer of lipotoxicity in in
vitro systems-or n-9, n-6, or n-3 unsaturated
fatty acids alone or in co-treatment with PAL, and measured the impact
of such treatments on ER stress and Muc2 production. Our results showed
that only eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids protect
goblet cells against ER stress-mediated altered Muc2 secretion induced
by PAL, whereas neither linolenic acid nor n-9 and n-6 FA
are able to provide such protection. We conclude that EPA and DHA could
represent potential therapeutic nutrients against the detrimental
lipotoxicity of saturated fatty acids, associated with type 2 diabetes
and obesity or inflammatory bowel disease. These in vitro data remain to
be explored in vivo in a context of dietary obesity.
KEYWORDS:
Muc2 secretion; endoplasmic reticulum stress; gastrointestinal barrier; intestinal goblet cells; n-3 fatty acids; palmitic acid
Muc2 secretion; endoplasmic reticulum stress; gastrointestinal barrier; intestinal goblet cells; n-3 fatty acids; palmitic acid
- PMID:
- 31514316
- PMCID:
- PMC6770956
- DOI:
- 10.3390/nu11092179
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